Date published: 2025-9-7

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Rad50 抗体 (13B3/2C6): sc-56209

5.0(3)
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说明书
  • Rad50 抗体 13B3/2C6 是小鼠单克隆 IgG1, 在7篇文献中引用,提供 50 µg/0.5 ml
  • 免疫human物种的Rad50的氨基酸1-425
  • 推荐用于 mouse, rat 和 human 来源的Rad50 WB, IP, IF 和 IHC(P)检测
  • 目前,我们还没有完成Rad50 抗体 (13B3/2C6)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

Rad52家族成员(Rad50、Rad51B/C/D、Rad52、Rad54、MRE11)介导DNA双链断裂修复(DSBR),以修复DNA损伤,否则会导致细胞死亡、突变或肿瘤转化。Rad51(RECA,BRCC5)与BRCA1和BRCA2相互作用,影响亚细胞定位和细胞对DNA损伤的反应。BRCA2失活可能是导致Rad51失调导致基因组不稳定和肿瘤发生的关键事件。Rad52形成七聚体环,结合单链DNA末端,催化互补链退火所需的DNA-DNA相互作用。DEAD样解旋酶超家族的Rad54A与双链DNA结合并诱导DNA拓扑变化,这被认为有助于同源DNA配对并刺激DNA重组。死亡样解旋酶超家族的Rad54B与双链DNA结合,并在DNA存在的情况下显示ATP酶活性。Rad54B在睾丸和脾脏中丰富,该基因突变发生在原发性淋巴瘤和结肠癌癌症中。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Rad50 抗体 (13B3/2C6) 参考文献:

  1. C1QBP 通过稳定 MRE11 和控制 MRE11/RAD50/NBS1 复合物的组装和激活促进同源重组  |  Bai, Y., et al. 2019. Mol Cell. 75: 1299-1314.e6. PMID: 31353207
  2. MRE11-RAD50-NBS1 复合物的改变和 DNA 损伤反应:对癌症治疗的影响。  |  Bian, L., et al. 2019. Mol Cancer. 18: 169. PMID: 31767017
  3. Mre11-Rad50 DNA断裂修复复合物的动态性质。  |  Beikzadeh, M. and Latham, MP. 2021. Prog Biophys Mol Biol. 163: 14-22. PMID: 33121960
  4. Rad50 通过激活 NF-κB 促进卵巢癌的进展。  |  Li, Y., et al. 2021. J Cell Mol Med. 25: 10961-10972. PMID: 34734468
  5. Mre11-Rad50 寡聚化促进了 DNA 双链断裂修复。  |  Kissling, VM., et al. 2022. Nat Commun. 13: 2374. PMID: 35501303
  6. Mre11-Rad50-Nbs1 复合物的低温电子显微镜结构揭示了支架功能的分子机制。  |  Rotheneder, M., et al. 2023. Mol Cell. 83: 167-185.e9. PMID: 36577401
  7. DNA 损伤应答 Mre11/Rad50 复合物的动态特性  |  Vertemara, J. and Tisi, R. 2023. Int J Mol Sci. 24: PMID: 37569756
  8. Xrs2/NBS1 促进了 MRE11-RAD50 复合物的末端桥接活性。  |  Möller, C., et al. 2024. Biochem Biophys Res Commun. 695: 149464. PMID: 38217957
  9. 小鼠生殖细胞中的 Rad50 基因突变会导致 DSB 形成减少、DSB 端部切除异常以及生殖细胞完全丧失。  |  Liu, Y., et al. 2024. Development. 151: PMID: 38512324
  10. Sae2/CtIP激活Mre11-Rad50内切酶活性的分子见解。  |  Nicolas, Y., et al. 2024. Mol Cell. 84: 2223-2237.e4. PMID: 38870937

订购信息

产品名称产品编号规格价格数量收藏夹

Rad50 抗体 (13B3/2C6)

sc-56209
50 µg/0.5 ml
RMB2377.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 2
Date published: 2017-02-17
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Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of Rad50 expression in HeLa, K-562, MCF7, Ramos, SW480 and Jurkat nuclear extracts.
Date published: 2017-01-20
Rated 5 out of 5 by from Strong band in WB in HeLaStrong band in WB in HeLa, Jurkat, and HEL 92.1.7 whole cell lysates. -SCBT QC
Date published: 2015-04-09
Rated 5 out of 5 by from Good WB antibody in HeLaGood WB antibody in HeLa, K-562, MCF7, Ramos, SW480, and Jurkat nuclear extracts. -SCBT QC
Date published: 2014-02-23
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Rad50 抗体 (13B3/2C6) is rated 5.0 out of 5 by 3.
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