Date published: 2025-9-7
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MDM2 Antibody (HDM2-323) - Western Blotting - Image 54445
MDM2 抗体 (HDM2-323): sc-56154. U-2 OS 全细胞裂解液中 MDM2 表达的 Western 印迹分析.
MDM2 Antibody (HDM2-323) - Western Blotting - Image 54445
MDM2 抗体 (HDM2-323): sc-56154. U-2 OS 全细胞裂解液中 MDM2 表达的 Western 印迹分析.
MDM2 Antibody (HDM2-323) - Western Blotting - Image 50256
MDM2 抗体 (HDM2-323): sc-56154. 对 Saos-2 全细胞裂解液中 MDM2 的表达进行 Western 印迹分析.
MDM2 抗体 (HDM2-323): sc-56154. U-2 OS 全细胞裂解液中 MDM2 表达的 Western 印迹分析.

MDM2 抗体 (HDM2-323): sc-56154

4.7(9)
寫評論提問

说明书
  • MDM2 抗体 HDM2-323 是小鼠单克隆 IgG2a, 在33篇文献中引用，提供 100 µg/ml
  • 免疫human物种的MDM2的氨基酸389-402
  • 推荐用于 mouse, rat 和 human 来源的MDM2 WB 和 IP检测
  • 看 MDM2 (SMP14): sc-965，可查看有标记的 MDM2 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取MDM2 (HDM2-323): sc-56154的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是MDM2 Antibody (HDM2-323) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与MDM2 Antibody (HDM2-323) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

MDM2抗体（HDM2-323）是一种IgG2a小鼠单克隆MDM2抗体，可通过WB和IP检测小鼠、大鼠和人的MDM2。MDM2抗体（HDM2-323）为非偶联的抗MDM2抗体形式。p53是目前为止在人类癌症中最常见的突变基因。p53的表达通过阻止细胞从G1期进入S期来抑制细胞生长。最重要的是，p53的功能是在细胞暴露于DNA损伤因子后，使细胞在细胞周期的G1期停滞。MDM2（鼠双微体2）蛋白最初是在鼠转化系统中被鉴定为致癌基因。MDM2的功能是与p53结合，并阻断p53介导的共转染报告基因构建的反式激活。MDM2基因在保留野生型p53的人类肉瘤中的扩增比例很高，而过表达MDM2的肿瘤细胞可以耐受高水平的p53表达。这些发现表明，MDM2的过表达至少是p53功能在肿瘤发生过程中被废除的一种机制。

仅限研究使用。不适用于诊断和治疗用途。

订购信息

产品名称产品编号规格价格数量收藏夹

MDM2 抗体 (HDM2-323)

sc-56154
100 µg/ml
RMB2377.00

MDM2 (HDM2-323): m-IgG Fc BP-HRP 套装

sc-539031
100 µg Ab; 10 µg BP
RMB2662.00

MDM2 (HDM2-323): m-IgG2a BP-HRP 套装

sc-546661
100 µg Ab; 10 µg BP
RMB2662.00

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 3
Date published: 2017-02-14
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Rated 5 out of 5 by from Great antibodyVery nice results from hippocampus tissue by WB analysis, in 1:500 dilution.
Date published: 2018-05-24
Rated 5 out of 5 by from Good for Western blotCapable of providing a clear and specific signal at the expected molecular weight. It also reacts well with mouse, rat, and human samples. This product has been used to publish several times.
Date published: 2017-02-07
Rated 5 out of 5 by from Works greatThis antibody works great for both human and mouse cell lysate. WB showed a clean band with little background (1:1000).
Date published: 2016-11-15
Rated 4 out of 5 by from Worked well in WBWorked well in WB
Date published: 2015-11-17
Rated 5 out of 5 by from Clean blot with single strong band at expectedClean blot with single strong band at expected size in Saos-2 whole cell lysate. -SCBT QC
Date published: 2015-03-22
Rated 4 out of 5 by from Nice publishable data on Western blot onNice publishable data on Western blot on human HEK293 cells, and LNCaP cells (human prostate carcinoma). -SCBT Publication Review
Date published: 2015-03-16
Rated 4 out of 5 by from Performs well in WesternPerforms well in Western, good signal with U-2 OS whole cell lysate. -SCBT QC
Date published: 2015-03-06
Rated 5 out of 5 by from Publishable Western blot results using human BCPublishable Western blot results using human BC-PAP, Hth-74, various thyroid cancer cells. -SCBT Publication Review
Date published: 2015-01-13
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MDM2 抗体 (HDM2-323) is rated 4.7 out of 5 by 9.
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