Date published: 2025-9-7
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MDM2 Antibody (D-7) - Western Blotting - Image 354365
MDM2 抗体 (D-7) HRP: sc-13161 HRP. 293T (A), U-2 OS (B), A-673 (C) 和 RAW 264. 7 (D) 全细胞裂解液中 MDM2 表达的直接 Western 印迹分析.
MDM2 抗体 (D-7) HRP: sc-13161 HRP. 293T (A), U-2 OS (B), A-673 (C) 和 RAW 264. 7 (D) 全细胞裂解液中 MDM2 表达的直接 Western 印迹分析.

MDM2 抗体 (D-7): sc-13161

4.8(9)
寫評論提問

说明书
  • MDM2抗体(D-7)是小鼠单克隆IgG2b κ, 在28篇文献中引用，规格为200 µg/ml
  • 免疫human物种的MDM2的氨基酸100-320
  • 抗-MDM2 抗体 (D-7) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 MDM2
  • 抗MDM2抗体（D-7）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 用于IF, IHC(P) 和FCM
  • 关于如何获取MDM2 (D-7): sc-13161的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2b BP-HRP 是 MDM2 抗体 (D-7) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MDM2 抗体 (D-7) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

MDM2抗体（D-7）是一种IgG2bκ小鼠单克隆MDM2抗体，可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类来源的MDM2。MDM2抗体（D-7）可作为非偶联抗MDM2抗体形式以及多种偶联形式的抗MDM2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。p53是迄今为止人类癌症中最常见的突变基因。p53的表达通过阻止细胞从细胞周期的G1期进展到S期来抑制细胞生长。最重要的是，p53在细胞暴露于DNA损伤剂后，在细胞周期的G1期引起细胞阻滞。MDM2（鼠双微体2）蛋白最初是在小鼠转化系统中被鉴定为癌基因。MDM2的功能是结合p53并阻断p53介导的共转染报告构建体的反式激活。在保留野生型p53的人肉瘤中，MDM2基因被高比例扩增，而过表达MDM2的肿瘤细胞可以耐受高水平的p53表达。这些发现表明，MDM2过表达至少代表了一种在肿瘤发生过程中p53功能被破坏的机制。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

MDM2 抗体 (D-7)

sc-13161
200 µg/ml
RMB2377.00

MDM2 (D-7): m-IgG Fc BP-HRP 套装

sc-525469
200 µg Ab; 10 µg BP
RMB2662.00

MDM2 (D-7): m-IgG2b BP-HRP 套装

sc-549564
200 µg Ab; 10 µg BP
RMB2662.00

MDM2 抗体 (D-7) AC

sc-13161 AC
500 µg/ml, 25% agarose
RMB3129.00

MDM2 抗体 (D-7) HRP

sc-13161 HRP
200 µg/ml
RMB2377.00

MDM2 抗体 (D-7) FITC

sc-13161 FITC
200 µg/ml
RMB2482.00

MDM2 抗体 (D-7) PE

sc-13161 PE
200 µg/ml
RMB2580.00

MDM2 抗体 (D-7) Alexa Fluor® 488

sc-13161 AF488
200 µg/ml
RMB2685.00

MDM2 抗体 (D-7) Alexa Fluor® 546

sc-13161 AF546
200 µg/ml
RMB2685.00

MDM2 抗体 (D-7) Alexa Fluor® 594

sc-13161 AF594
200 µg/ml
RMB2685.00

MDM2 抗体 (D-7) Alexa Fluor® 647

sc-13161 AF647
200 µg/ml
RMB2685.00

MDM2 抗体 (D-7) Alexa Fluor® 680

sc-13161 AF680
200 µg/ml
RMB2685.00

MDM2 抗体 (D-7) Alexa Fluor® 790

sc-13161 AF790
200 µg/ml
RMB2685.00

MDM2 抗体 (D-7) TRITC

sc-13161 TRITC
200 µg/ml
RMB2407.00

Can MDM2 (D-7): sc-13161 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, MDM2 (D-7): sc-13161 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Great results on canine testis with IHCTested this antibody on a canine testis (control tissue). Works perfectly at pH 9.0 with a 1:100 dilution.
Date published: 2022-08-09
Rated 5 out of 5 by from Great antibodyVery nice results from hippocampus tissue by WB analysis, in 1:500 dilution.
Date published: 2018-05-24
Rated 5 out of 5 by from Full Length and Cleaved Forms detectedThis antibody shows clear detection of both the full length MDM2 and the cleaved form of the protein in Western Blot.
Date published: 2017-01-26
Rated 5 out of 5 by from Good WB publishable data gathered with humanGood WB publishable data gathered with human breast carcinoma cell line. -SCBT Publication Review
Date published: 2015-04-19
Rated 5 out of 5 by from Published in IP on human MIAPublished in IP on human MIA-PaCa-2 cells. Nice data! -SCBT Publication Review
Date published: 2015-03-16
Rated 4 out of 5 by from Performs well in WesternPerforms well in Western, good signal with MCF7 whole cell lysate. -SCBT QC
Date published: 2015-02-21
Rated 4 out of 5 by from This antibody works well and can be usedThis antibody works well and can be used for WB with human H1299 (lung carcinoma) cells. -SCBT Publication Review
Date published: 2015-01-20
Rated 5 out of 5 by from Great antibody for IPGreat antibody for IP, published using MCF 7 cells. -SCBT Publication Review
Date published: 2014-04-15
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MDM2 抗体 (D-7) is rated 4.8 out of 5 by 9.
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