Date published: 2025-9-8

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Visual Arrestin 抗体 (E-3): sc-166383

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说明书
  • Visual Arrestin 抗体 E-3 是小鼠单克隆 IgG2b κ,Visual Arrestin抗体, 在8篇文献中引用,规格为200 µg/ml
  • 针对氨基酸316-405的抗原,在Visual Arrestin的C-terminus处映射,human来源
  • Visual Arrestin 抗体 (E-3) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Visual Arrestin
  • 抗Visual Arrestin抗体(E-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Visual Arrestin (E-3): sc-166383免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRPm-IgGκ BP-HRP 是 Visual Arrestin 抗体 (E-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Visual Arrestin 抗体 (E-3) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

视觉抑制蛋白抗体(E-3)是一种IgG2b κ小鼠单克隆视觉抑制蛋白抗体(也称为SAG抗体或S-抗原视觉抑制蛋白抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类的视觉抑制蛋白。视觉抑制蛋白抗体(E-3)既可以是非偶联的抗视觉抑制蛋白抗体形式,也可以是多种偶联形式的抗视觉抑制蛋白抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Arrestin/β-Arrestin蛋白家族的成员被认为参与G蛋白偶联受体的激动剂介导脱敏,并引起细胞对激素、神经递质或感觉信号等刺激的特异性减弱应答。视觉抑制蛋白,也称为Arrestin、视网膜S-抗原或S-Arrestin,是一种主要的可溶性光感受器蛋白,通过G蛋白偶联受体(视紫红质)的激活调节光依赖性信号转导。视觉抑制蛋白在视网膜光感受器细胞和松果体中表达。视觉抑制蛋白是炎症性眼病的主要致病自身抗原,如葡萄膜炎和小口病,后者是一种罕见的常染色体隐性夜盲症。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Visual Arrestin 抗体 (E-3) 参考文献:

  1. 日本小口病患者的基因分析和单基因创始效应评估。  |  Saga, M., et al. 2004. Jpn J Ophthalmol. 48: 350-2. PMID: 15295660
  2. 用纯化的重组人视网膜 S 抗原融合蛋白诱导 Lewis 大鼠患上实验性自身免疫性葡萄膜视网膜炎  |  Roberts, AJ., et al. 1992. Eur J Immunol. 22: 951-6. PMID: 1551407
  3. 人类 S 抗原基因的结构组织。cDNA, 氨基酸, 内含子, 外显子, 启动子, 体外转录, 视网膜和松果体。  |  Yamaki, K., et al. 1990. J Biol Chem. 265: 20757-62. PMID: 2249983
  4. 用单克隆抗体分析视网膜 S 抗原的抗原决定因子。  |  Banga, JP., et al. 1988. Invest Ophthalmol Vis Sci. 29: 12-21. PMID: 2447030
  5. arrestin 对红细胞蛋白去磷酸化的调控。  |  Palczewski, K., et al. 1989. J Biol Chem. 264: 15770-3. PMID: 2550422

订购信息

产品名称产品编号规格价格数量收藏夹

Visual Arrestin 抗体 (E-3)

sc-166383
200 µg/ml
RMB2377.00

Visual Arrestin (E-3): m-IgGκ BP-HRP 套装

sc-521583
200 µg Ab, 40 µg BP
RMB2662.00

Visual Arrestin (E-3): m-IgG2b BP-HRP 套装

sc-548932
200 µg Ab; 10 µg BP
RMB2662.00

Visual Arrestin 抗体 (E-3) AC

sc-166383 AC
500 µg/ml, 25% agarose
RMB3129.00

Visual Arrestin 抗体 (E-3) HRP

sc-166383 HRP
200 µg/ml
RMB2377.00

Visual Arrestin 抗体 (E-3) FITC

sc-166383 FITC
200 µg/ml
RMB2482.00

Visual Arrestin 抗体 (E-3) PE

sc-166383 PE
200 µg/ml
RMB2580.00

Visual Arrestin 抗体 (E-3) Alexa Fluor® 488

sc-166383 AF488
200 µg/ml
RMB2685.00

Visual Arrestin 抗体 (E-3) Alexa Fluor® 546

sc-166383 AF546
200 µg/ml
RMB2685.00

Visual Arrestin 抗体 (E-3) Alexa Fluor® 594

sc-166383 AF594
200 µg/ml
RMB2685.00

Visual Arrestin 抗体 (E-3) Alexa Fluor® 647

sc-166383 AF647
200 µg/ml
RMB2685.00

Visual Arrestin 抗体 (E-3) Alexa Fluor® 680

sc-166383 AF680
200 µg/ml
RMB2685.00

Visual Arrestin 抗体 (E-3) Alexa Fluor® 790

sc-166383 AF790
200 µg/ml
RMB2685.00

Does this antibody react with S-arrestin or arrestin 1. Its not stated in the antibody specific sheet.

Asked by: sq wang
Thank you for your question. This antibody is specific for S-arrestin. On the product data sheet and on the website it indicates the specific gene and protein info regarding the reactivity of the antibody. Genetic locus: SAG (human) mapping to 2q37.1; Sag (mouse) mapping to 1 D.
Answered by: Technical Support
Date published: 2019-08-17

I am using Visual Arrestin (E-3): sc-166383 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Good specificity shown by using transfectedGood specificity shown by using transfected and non-transfected 293T cells for over-expression of the mouse Visual Arrestin protein via Western Blot analysis. -SCBT QC
Date published: 2015-05-19
Rated 5 out of 5 by from Produced excellent immunofluorescence cytoplasmicProduced excellent immunofluorescence cytoplasmic staining in methanol-fixed NIH/3T3 cells. -SCBT QC
Date published: 2015-05-07
Rated 4 out of 5 by from Positive detection of Visual Arrestin proteinPositive detection of Visual Arrestin protein in rat eye tissue using WB analysis. -SCBT QC
Date published: 2015-05-01
Rated 4 out of 5 by from An increase in signal was observed via WesternAn increase in signal was observed via Western Blot when comparing 293 cells transfected with human Visual Arrestin protein with those that have not. -SCBT QC
Date published: 2015-03-26
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Visual Arrestin 抗体 (E-3) is rated 4.5 out of 5 by 4.
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