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β-Arrestin-2 抗体 (H-9): sc-13140

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说明书
  • β-Arrestin-2抗体(H-9)是小鼠单克隆IgG1 κ, 在99篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的β-Arrestin-2的N-terminus的氨基酸2-33之间
  • 抗-beta-Arrestin-2 抗体 (H-9) 推荐用于 WB, IP, IF, IHC(P), FCM 和 ELISA,检测mouse, rat 和human 来源的 β-Arrestin-2; 也和以下物种反应,包括: 和 canine, bovine and porcine
  • 还可偶联藻红蛋白 用于IF, IHC(P) 和 FCM
  • beta-Arrestin-2抗体(H-9)可与Alexa Fluor® 546Alexa Fluor® 594结合,用于WB(RGB)、IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是β-Arrestin-2 Antibody (H-9) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与β-Arrestin-2 Antibody (H-9) 打包提供(请参阅下面的订购信息)。

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関連項目

β-Arrestin-2抗体(H-9)是一种小鼠单克隆IgG1 kappa轻链抗体,专门设计用于检测包括人类、小鼠、大鼠、犬、牛和猪在内的多种物种中的β-Arrestin-2(也称为ARRB2)。抗-β-Arrestin-2抗体(H-9)可识别人类β-Arrestin-2蛋白N端2-33氨基酸之间的表位,确保在应用中可靠检测,如蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)、流式细胞术(FCM)和酶联免疫吸附测定(ELISA)。 石蜡包埋切片(IHCP)、流式细胞术(FCM)和酶联免疫吸附测定(ELISA)等应用中。β-Arrestin-2是G蛋白偶联受体(GPCR)信号通路的关键调节因子,可调节受体的脱敏、内化,并启动独立于G蛋白的替代信号级联反应。这种多功能蛋白主要在神经元和脾脏组织中表达,β-Arrestin-2在调节神经递质受体活性以及细胞对各种信号分子的反应方面发挥着重要作用。β-Arrestin-2能够影响多种生理过程,因此成为神经传递、炎症和癌症进展等领域研究的关键目标。了解β-Arrestin-2的相互作用和调节机制对于开发针对这些关键途径的治疗策略至关重要。β-Arrestin-2 (H-9)抗体有结合和非结合两种形式,具有多功能性,可满足不同的实验需求。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

β-Arrestin-2 抗体 (H-9) 参考文献:

  1. beta-arrestin2,捕获素/beta-arrestin 基因家族的新成员。  |  Attramadal, H., et al. 1992. J Biol Chem. 267: 17882-90. PMID: 1517224
  2. 两种激酶介导了依赖于激动剂的 beta 2-肾上腺素能受体的磷酸化和脱敏。  |  Hausdorff, WP., et al. 1990. Symp Soc Exp Biol. 44: 225-40. PMID: 1983369
  3. 真核细胞中表达的α-肾上腺素能受体亚型的多种第二信使途径。  |  Cotecchia, S., et al. 1990. J Biol Chem. 265: 63-9. PMID: 2152928
  4. β-Arrestin-2 BRET 生物传感器检测与 GPCRs 相互作用的不同 β-Arrestin-2 构象。  |  Oishi, A., et al. 2020. ACS Sens. 5: 57-64. PMID: 31849219
  5. β-arrestin 2作为cGAS-STING信号的激活剂和病毒免疫逃逸的靶标。  |  Zhang, Y., et al. 2020. Nat Commun. 11: 6000. PMID: 33243993
  6. β-Arrestin 2 通过 ERK1/2 信号调控针对结核分枝杆菌感染的炎症反应  |  Wen, Q., et al. 2021. J Immunol. 206: 2623-2637. PMID: 34001657
  7. β-arrestin-1和β-arrestin-2抑制MRGPRX2引发的人类皮肤肥大细胞脱颗粒和ERK1/2激活。  |  Wang, Z., et al. 2022. Front Allergy. 3: 930233. PMID: 35910860
  8. NE激活的β2-AR/β-arrestin 2/Src通路介导了水浸限制应激引起的十二指肠高渗透性。  |  Yan, JT., et al. 2023. Am J Physiol Cell Physiol. 324: C133-C141. PMID: 36440855
  9. GLP-1和GIP受体通过不同的β-arrestin 2依赖性途径发出信号,以调节胰腺β细胞功能。  |  Zaïmia, N., et al. 2023. Cell Rep. 42: 113326. PMID: 37897727
  10. G 蛋白偶联受体超家族中保守的七跨膜序列 NP(X)2,3Y 调节着 beta 2-肾上腺素能受体的多种特性。  |  Barak, LS., et al. 1995. Biochemistry. 34: 15407-14. PMID: 7492540
  11. Arrestin 在体内 G 蛋白偶联受体 rhodopsin 失活过程中的功能。  |  Dolph, PJ., et al. 1993. Science. 260: 1910-6. PMID: 8316831
  12. 人类 beta-arrestin-1 的分子分析:克隆, 组织分布和表达调控。鉴定由替代剪接产生的两种异构体。  |  Parruti, G., et al. 1993. J Biol Chem. 268: 9753-61. PMID: 8486659

订购信息

产品名称产品编号规格价格数量收藏夹

β-Arrestin-2 抗体 (H-9)

sc-13140
200 µg/ml
RMB2377.00

β-Arrestin-2 (H-9): m-IgGκ BP-HRP 套装

sc-520604
200 µg Ab, 40 µg BP
RMB2662.00

β-Arrestin-2 (H-9): m-IgG1 BP-HRP 套装

sc-533467
200 µg Ab; 20 µg BP
RMB2662.00

β-Arrestin-2 抗体 (H-9) PE

sc-13140 PE
200 µg/ml
RMB2580.00

β-Arrestin-2 抗体 (H-9) Alexa Fluor® 546

sc-13140 AF546
200 µg/ml
RMB2685.00

β-Arrestin-2 抗体 (H-9) Alexa Fluor® 594

sc-13140 AF594
200 µg/ml
RMB2685.00

β-Arrestin-2 抗体 (H-9) Alexa Fluor® 680

sc-13140 AF680
200 µg/ml
RMB2685.00

β-Arrestin-2 抗体 (H-9) Alexa Fluor® 790

sc-13140 AF790
200 µg/ml
RMB2685.00

β-Arrestin-2 (H-9) 中和勝肽

sc-13140 P
100 µg/0.5 ml
RMB511.00

What is the recommended secondary reagent for Western blot in human samples?

Asked by: Trav11
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent, such as m-IgGκ BP-HRP: sc-516102, for Western blot. A complete list of all available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety If you have any further questions or concerns, please feel free to contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or using the Live Chat function on our website.
Answered by: Tech Service 8
Date published: 2016-12-30
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Rated 5 out of 5 by from Clean, strong signalGreat antibody performance across the board in Western Blot, IF, IHC and Flow.
Date published: 2017-01-12
Rated 4 out of 5 by from WB: Worked well in rat AtTWB: Worked well in rat AtT-20. -SCBT Publication Review
Date published: 2015-04-17
Rated 4 out of 5 by from Performs well in WesternPerforms well in Western, good signal with HEY and OVCA 433 cells -SCBT Publication Review
Date published: 2015-04-05
Rated 5 out of 5 by from Strong signal achieved in WB with RAW 264Strong signal achieved in WB with RAW 264.7 and PC-12 whole cell lysates. -SCBT QC
Date published: 2015-04-03
Rated 5 out of 5 by from Good WB publishable data gathered with humanGood WB publishable data gathered with human HEK293B2. -SCBT Publication Review
Date published: 2015-04-03
Rated 4 out of 5 by from Antibody was published for use in ImmunoprecipitationAntibody was published for use in Immunoprecipitation on embryonic kidney HEK293 cells. Worked well! -SCBT Publication Review
Date published: 2015-03-31
Rated 5 out of 5 by from Published data for IF in human SHPublished data for IF in human SH-SY5Y cells -SCBT Publication Review
Date published: 2015-03-15
Rated 5 out of 5 by from Publishable Western blot results using MEFsPublishable Western blot results using MEFs. -SCBT Publication Review
Date published: 2015-03-11
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β-Arrestin-2 抗体 (H-9) is rated 4.6 out of 5 by 13.
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