Date published: 2025-9-8
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Von Hippel Lindau/VHL Antibody (VHL40) - Western Blotting - Image 353345
VHL 抗体 (VHL40): sc-135657. 在 HeLa (A), Jurkat (B), Daudi (C), HEK293 (D) 和 Caco-2 (E) 全细胞裂解液及人胎盘组织提取物 (F) 中 VHL 表达的 Western 印迹分析.
VHL 抗体 (VHL40): sc-135657. 在 HeLa (A), Jurkat (B), Daudi (C), HEK293 (D) 和 Caco-2 (E) 全细胞裂解液及人胎盘组织提取物 (F) 中 VHL 表达的 Western 印迹分析.

Von Hippel Lindau/VHL 抗体 (VHL40): sc-135657

4.5(17)
寫評論提問

说明书
  • Von Hippel Lindau/VHL 抗体 VHL40 是小鼠单克隆 IgG1 κ，Von Hippel Lindau/VHL抗体, 在28篇文献中引用，规格为200 µg/ml
  • 免疫human物种的VHL的氨基酸54-213
  • Von Hippel Lindau/VHL 抗体 (VHL40) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 VHL
  • 抗Von Hippel Lindau/VHL抗体（VHL40）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Von Hippel Lindau/VHL (VHL40): sc-135657的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 Von Hippel Lindau/VHL 抗体 (VHL40) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与Von Hippel Lindau/VHL 抗体 (VHL40) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

VHL抗体（VHL40）是一种IgG1 κ小鼠单克隆VHL抗体（也称为VHL抗体或von Hippel-Lindau综合征抗体），它通过蛋白质印迹WB、免疫沉淀IP、IF和IHC（P）等方法检测小鼠、大鼠和人类的VHL蛋白。VHL抗体（VHL40）既可作为非偶联的抗VHL抗体形式提供，也可作为多种偶联形式的抗VHL抗体提供，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。在肿瘤抑制基因von Hippel-Lindau（VHL）中携带种系突变的个体对包括肾癌、中枢神经系统血管母细胞瘤和嗜铬细胞瘤在内的多种肿瘤的易感性增加。已确定Elongin（SIII）复合物为VHL蛋白的功能靶点。Elongin（SIII）是一个由转录活性亚基Elongin A和两个调节亚基Elongin B和Elongin C组成的异三聚体。VHL通过与Elongin B和C亚基结合来发挥功能，抑制Elongin（SIII）复合物的转录效率。已观察到VHL的不同亚型，由选择性剪接的转录变异体编码。每种亚型的分子量在物种之间有所不同。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Von Hippel Lindau/VHL 抗体 (VHL40) 参考文献:

  1. von Hippel-Lindau 综合征肾细胞癌 3p 上的杂合性缺失。  |  Decker, HJ., et al. 1989. Cancer Genet Cytogenet. 39: 289-93. PMID: 2568884
  2. 与 von Hippel-Lindau 病相关的肿瘤中的特定基因变化。  |  Tory, K., et al. 1989. J Natl Cancer Inst. 81: 1097-101. PMID: 2738940
  3. Von Hippel-Lindau 病与肾细胞癌相关的第 3 号染色体区域有关。  |  Seizinger, BR., et al. 1988. Nature. 332: 268-9. PMID: 2894613
  4. SOCS和VHL蛋白在神经元分化和发展中的作用。  |  Kanno, H., et al. 2023. Int J Mol Sci. 24: PMID: 36835292
  5. FBXO22通过介导VHL泛素化和降解促进胶质母细胞瘤的恶性进展。  |  Shen, Z., et al. 2024. Cell Death Discov. 10: 151. PMID: 38519492
  6. 具有增强效力的肽模拟VHL配体，具有改善的口服生物利用度。  |  Wu, H., et al. 2024. J Med Chem. 67: 8585-8608. PMID: 38809766
  7. 肿瘤抑制和转录延伸：改变合作伙伴的可怕后果。  |  Krumm, A. and Groudine, M. 1995. Science. 269: 1400-1. PMID: 7660121
  8. VHL 肿瘤抑制蛋白对转录延伸的抑制。  |  Duan, DR., et al. 1995. Science. 269: 1402-6. PMID: 7660122
  9. Elongin (SIII)：RNA 聚合酶 II 延长过程的多亚基调节器。  |  Aso, T., et al. 1995. Science. 269: 1439-43. PMID: 7660129
  10. RNA 聚合酶 II 延长因子 SIII 重要亚基的分子克隆。  |  Garrett, KP., et al. 1994. Proc Natl Acad Sci U S A. 91: 5237-41. PMID: 8202474
  11. 与 von Hippel-Lindau 基因新型突变有关的家族性嗜铬细胞瘤。  |  Gross, DJ., et al. 1996. J Clin Endocrinol Metab. 81: 147-9. PMID: 8550742
  12. 在头颈癌中，染色体臂 3p 的等位基因频繁缺失与 Von-Hippel Lindau 抑癌基因的基因改变不同。  |  Waber, PG., et al. 1996. Oncogene. 12: 365-9. PMID: 8570213

订购信息

产品名称产品编号规格价格数量收藏夹

Von Hippel Lindau/VHL 抗体 (VHL40)

sc-135657
200 µg/ml
$316.00

Von Hippel Lindau/VHL (VHL40): m-IgGκ BP-HRP 套装

sc-521297
200 µg Ab, 40 µg BP
$354.00

Von Hippel Lindau/VHL 抗体 (VHL40) AC

sc-135657 AC
500 µg/ml, 25% agarose
$416.00

Von Hippel Lindau/VHL 抗体 (VHL40) HRP

sc-135657 HRP
200 µg/ml
$316.00

Von Hippel Lindau/VHL 抗体 (VHL40) FITC

sc-135657 FITC
200 µg/ml
$330.00

Von Hippel Lindau/VHL 抗体 (VHL40) PE

sc-135657 PE
200 µg/ml
$343.00

Von Hippel Lindau/VHL 抗体 (VHL40) Alexa Fluor® 488

sc-135657 AF488
200 µg/ml
$357.00

Von Hippel Lindau/VHL 抗体 (VHL40) Alexa Fluor® 546

sc-135657 AF546
200 µg/ml
$357.00

Von Hippel Lindau/VHL 抗体 (VHL40) Alexa Fluor® 594

sc-135657 AF594
200 µg/ml
$357.00

Von Hippel Lindau/VHL 抗体 (VHL40) Alexa Fluor® 647

sc-135657 AF647
200 µg/ml
$357.00

Von Hippel Lindau/VHL 抗体 (VHL40) Alexa Fluor® 680

sc-135657 AF680
200 µg/ml
$357.00

Von Hippel Lindau/VHL 抗体 (VHL40) Alexa Fluor® 790

sc-135657 AF790
200 µg/ml
$357.00

A strong band is shown at 19kDa. Can this antibody show another size at 30kDa?

Asked by: Daqiang
Thank you for your question. Please contact technical service for assistance. Please email [email protected] or call (800)-457-3801.
Answered by: BlakeJ
Date published: 2025-03-27

Hello, I need to stain cells by flow cytometry, do you know if this conjugated antibody will work? Thank you

Asked by: laurie38
Thank you for your question. We offer several fluorescence-conjugated versions of Anti-Von Hippel Lindau/VHL Antibody (VHL40): sc-135657 that can be used in flow cytometry.
Answered by: Tech Support Europe
Date published: 2022-09-05

There are two bands(19KD and 25KD) in my WB result, my sample is HEK293 cells whole lysate. Is it right? and Why?

Asked by: May1
感谢您的提问。如果您能直接拨打(+86) 021-60936350，或者发邮件到我们亚洲服务部的邮箱[email protected]我们就这个问题和其他相关问题进行更多的互动讨论，那将会很有帮助。 Thank you for your question.It would be helpful if you could call us directly at (+86) 021-60936350 or email our Asia Service department at [email protected] for more interactive discussions on this and other related issues.
Answered by: Zhanglong
Date published: 2021-09-02

I need to use an antibody to detect the protein in samples of bovine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 15
Date published: 2017-01-20
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Rated 5 out of 5 by from Detection of endogenous VHLStrong signal for endogenous VHL in HeLa and HEK293T cells. Dilution 1:1000.
Date published: 2024-09-30
Rated 5 out of 5 by from ottimo funzionamentoho comprato questo anticorpo un mese fa e sono molto soddisfatta
Date published: 2023-12-06
Rated 4 out of 5 by from work wellThere are two bands(19KD and 25KD) in my WB result, my sample is HEK293 cells whole lysate. Is there anyone has the similar results?
Date published: 2021-09-01
Rated 5 out of 5 by from Works well in WB for CHO cellsClear bands with minimal background signal in CHO cells
Date published: 2020-01-15
Rated 5 out of 5 by from VHL antibody in mouse heart tissues works very welVHL antibody in mouse heart tissues works very well. The size is about 21KD.
Date published: 2018-12-03
Rated 5 out of 5 by from Sehr gute Ergebnisse im Western BlotAls Sample angefordert und sofort die erwartete Bande bei ca. 20 kDa im Western Blot detektiert. Hat super funktioniert.
Date published: 2018-11-09
Rated 3 out of 5 by from santa也是老牌子了santa也是老牌子了，主要是产品的包装有待改善，做个包装看起来正规一些，没有用过santa的客户看到包装的瞬间体验感会差很多
Date published: 2018-02-23
Rated 2 out of 5 by from Dod not work for WB at 1:500 dilutionWe used a vial of sample product. Surprisingly, it can not detect VHL from 40 mcg K562 whole cell lysate at 1:500 dilution.
Date published: 2018-02-04
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Von Hippel Lindau/VHL 抗体 (VHL40) is rated 4.5 out of 5 by 17.
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