Date published: 2025-9-8

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V-ATPase C1 抗体 (G-5): sc-271077

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说明书
  • V-ATPase C1 抗体 G-5 是小鼠单克隆 IgG2b κ,V-ATPase C1抗体, 在4篇文献中引用,规格为200 µg/ml
  • 免疫human物种的V-ATPase C1的氨基酸83-382
  • V-ATPase C1 抗体 (G-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 V-ATPase C1
  • 抗V-ATPase C1抗体(G-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取V-ATPase C1 (G-5): sc-271077免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 V-ATPase C1 抗体 (G-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与V-ATPase C1 抗体 (G-5) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品,请参阅我们的小鼠 IgG 结合蛋白完整列表。

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関連項目

V-ATPase C1 抗体 (G-5)是一种IgG2b κ型小鼠单克隆V-ATPase C1抗体(也称V-ATPase C1抗体),通过WB、IP、IF、IHC(P)和ELISA等方法检测小鼠、大鼠和人源的V-ATPase C1蛋白。V-ATPase C1 Antibody (G-5)既可以是抗V-ATPase C1抗体的非偶联形式,也可以是多种偶联形式的抗V-ATPase C1抗体,包括琼脂糖,HRP, PE, FITC和多个Alexa Fluor®偶联物。空泡型H+- ATP酶(V-ATPase)是一种负责真核细胞内细胞器酸化的多亚基酶。V-ATP酶通过电化学梯度泵送质子,而F-ATP酶则逆转这一过程,从而合成ATP。负责ATP水解的外周V1结构域和负责质子易位的积分V0结构域组成V-ATP酶。V1结构域由9个亚基(A-H)组成,V0结构域由5个亚基(a、d、c、c'和c")组成。与F-ATP酶一样,V-ATP酶很可能通过旋转机制发挥作用。V-ATP酶C是一种普遍表达的辅助亚基。编码人V-ATP酶C的基因定位于染色体8q22.3。V-ATP酶D是另一个辅助亚基。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

V-ATPase C1 抗体 (G-5) 参考文献:

  1. 人类下丘脑-垂体-肾上腺轴的基因表达谱分析和全长 cDNA 克隆。  |  Hu, RM., et al. 2000. Proc Natl Acad Sci U S A. 97: 9543-8. PMID: 10931946
  2. 液泡(H+)-ATP 酶--自然界中用途最广的质子泵。  |  Nishi, T. and Forgac, M. 2002. Nat Rev Mol Cell Biol. 3: 94-103. PMID: 11836511
  3. 口腔鳞状细胞癌中的细胞内pH调节是通过ATP6V1C1基因的过度表达来增加V-ATP酶活性来介导的。  |  Otero-Rey, EM., et al. 2008. Oral Oncol. 44: 193-9. PMID: 17467328
  4. Atp6v1c1是破骨细胞质子泵和破骨细胞中F-肌动蛋白环形成的重要组成部分。  |  Feng, S., et al. 2009. Biochem J. 417: 195-203. PMID: 18657050
  5. 在口腔鳞状细胞癌中,ATP6V1C1在刷细胞学样本中的表达水平可作为诊断和预后指标。  |  Pérez-Sayáns, M., et al. 2010. Cancer Biol Ther. 9: 1057-64. PMID: 20404513
  6. 编码牛绒毛膜颗粒中 H(+)-ATP 酶 C 亚基的 cDNA 分子克隆。  |  Nelson, H., et al. 1990. J Biol Chem. 265: 20390-3. PMID: 2147024
  7. 靶向Atp6v1c1可预防牙周炎引起的炎症和骨侵蚀,并揭示其在骨免疫学中的关键功能。  |  Li, S., et al. 2015. PLoS One. 10: e0134903. PMID: 26274612
  8. 口腔癌发生过程中ATP6V1C1的表达。  |  Oliveira Alves, MG., et al. 2016. Biotech Histochem. 91: 263-8. PMID: 26984774
  9. MicroRNA29a通过调节ATP酶H⁺转运V1亚基C1,逆转肝星状细胞在肝纤维化消退中的作用。  |  Jing, F., et al. 2019. Int J Mol Sci. 20: PMID: 30781750
  10. ATP6V1C1的ATP酶亚基可抑制食管鳞状细胞癌的细胞自噬,增强其放射治疗耐受性。  |  Yao, X., et al. 2021. Gene. 768: 145261. PMID: 33183740
  11. ATP6V1C1与肿瘤微环境和mTORC1信号通路相关,是肝癌的潜在诊断、预后和治疗生物标志物。  |  Pan, Y., et al. 2024. Discov Oncol. 15: 673. PMID: 39557733
  12. 人类空泡 H(+)-ATPase 亚基 C, D 和 E 的克隆及组织分布。  |  van Hille, B., et al. 1993. Biochem Biophys Res Commun. 197: 15-21. PMID: 8250920

订购信息

产品名称产品编号规格价格数量收藏夹

V-ATPase C1 抗体 (G-5)

sc-271077
200 µg/ml
RMB2377.00

V-ATPase C1 (G-5): m-IgGκ BP-HRP 套装

sc-521841
200 µg Ab, 40 µg BP
RMB2662.00

V-ATPase C1 抗体 (G-5) AC

sc-271077 AC
500 µg/ml, 25% agarose
RMB3129.00

V-ATPase C1 抗体 (G-5) HRP

sc-271077 HRP
200 µg/ml
RMB2377.00

V-ATPase C1 抗体 (G-5) FITC

sc-271077 FITC
200 µg/ml
RMB2482.00

V-ATPase C1 抗体 (G-5) PE

sc-271077 PE
200 µg/ml
RMB2580.00

V-ATPase C1 抗体 (G-5) Alexa Fluor® 488

sc-271077 AF488
200 µg/ml
RMB2685.00

V-ATPase C1 抗体 (G-5) Alexa Fluor® 546

sc-271077 AF546
200 µg/ml
RMB2685.00

V-ATPase C1 抗体 (G-5) Alexa Fluor® 594

sc-271077 AF594
200 µg/ml
RMB2685.00

V-ATPase C1 抗体 (G-5) Alexa Fluor® 647

sc-271077 AF647
200 µg/ml
RMB2685.00

V-ATPase C1 抗体 (G-5) Alexa Fluor® 680

sc-271077 AF680
200 µg/ml
RMB2685.00

V-ATPase C1 抗体 (G-5) Alexa Fluor® 790

sc-271077 AF790
200 µg/ml
RMB2685.00

I am using V-ATPase C1 (G-5): sc-271077 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Germaine
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from 免疫沈降で使用Immunoprecipitationで使用。5ul/sampleで用いた。IPできているものの、あまり強くはない。
Date published: 2020-06-19
Rated 5 out of 5 by from Excellent immunoperoxidase cytoplasmic stainingExcellent immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human esophagus tissue. -SCBT QC
Date published: 2015-04-29
Rated 5 out of 5 by from Endogenous levels of this protein detectedEndogenous levels of this protein detected using product sc-271077 by WB on 293T (non-transfected), HeLa and HepG2 whole cell lysates. -SCBT QC
Date published: 2015-04-02
Rated 5 out of 5 by from Good specificity shown by using transfectedGood specificity shown by using transfected and non-transfected 293T cells for over-expression of the mouse V-ATPase C1 protein via Western Blot analysis. -SCBT QC
Date published: 2015-03-06
Rated 5 out of 5 by from Positive detection of endogenous expression of VPositive detection of endogenous expression of V-ATPase C1 in SK-N-MC whole cell lysate, mouse kidney tissue extract and rat brain tissue extract. -SCBT QC
Date published: 2015-01-14
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V-ATPase C1 抗体 (G-5) is rated 4.6 out of 5 by 5.
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