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SERCA2 Antibody (IID8) - Western Blotting - Image 354048
SERCA2 抗体 (IID8): sc-53010. A-431 (A), HT-1080 (B), K-562 (C), CCRF-CEM (D) 和 Hep G2 (E) 全细胞裂解液中 SERCA2 表达的 Western 印迹分析.
SERCA2 抗体 (IID8): sc-53010. A-431 (A), HT-1080 (B), K-562 (C), CCRF-CEM (D) 和 Hep G2 (E) 全细胞裂解液中 SERCA2 表达的 Western 印迹分析.

SERCA2 抗体 (IID8): sc-53010

4.1(7)
寫評論提問

说明书
  • SERCA2抗体(IID8)是小鼠单克隆IgG1 κ, 在24篇文献中引用，规格为200 µg/ml
  • 针对纯化的canine心脏肌浆网
  • 抗-SERCA2 抗体 (IID8) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 SERCA2; 也和以下物种反应，包括： 和 rabbit, bovine, canine and porcine
  • 抗SERCA2抗体（IID8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SERCA2 (IID8): sc-53010的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 SERCA2 抗体 (IID8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与SERCA2 抗体 (IID8) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 SERCA 抗体 包括 SERCA1, SERCA2和SERCA3

SERCA2抗体（IID8）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过应用诸如蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学等方法检测小鼠、大鼠和人类样本中的SERCA2。抗SERCA2抗体（IID8）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。SERCA2通过调节钙离子进入肌浆网（肌肉收缩和放松所必需的）来维持钙离子平衡，从而发挥关键作用。这一过程可维持细胞质中较低的钙离子浓度，防止肌肉过度收缩，确保心脏功能正常。SERCA2经历组织特异性加工，形成两种同工型：SERCA2a主要存在于I型（慢速）骨骼肌、心肌和平滑肌中，而SERCA2b则存在于多种细胞类型中。SERCA2对钙水平的调节尤其会影响心肌的收缩和舒张，凸显其在心血管健康中的重要性。SERCA2（IID8）单克隆抗体是研究人员研究钙信号和肌肉生理学的宝贵工具，有助于深入了解肌肉功能和相关疾病的分子机制。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

SERCA2 抗体 (IID8)

sc-53010
200 µg/ml
RMB2377.00

SERCA2 (IID8): m-IgG Fc BP-HRP 套装

sc-526758
200 µg Ab; 10 µg BP
RMB2662.00

SERCA2 (IID8): m-IgG1 BP-HRP 套装

sc-532131
200 µg Ab; 20 µg BP
RMB2662.00

SERCA2 抗体 (IID8) AC

sc-53010 AC
500 µg/ml, 25% agarose
RMB3129.00

SERCA2 抗体 (IID8) HRP

sc-53010 HRP
200 µg/ml
RMB2377.00

SERCA2 抗体 (IID8) FITC

sc-53010 FITC
200 µg/ml
RMB2482.00

SERCA2 抗体 (IID8) PE

sc-53010 PE
200 µg/ml
RMB2580.00

SERCA2 抗体 (IID8) Alexa Fluor® 488

sc-53010 AF488
200 µg/ml
RMB2685.00

SERCA2 抗体 (IID8) Alexa Fluor® 546

sc-53010 AF546
200 µg/ml
RMB2685.00

SERCA2 抗体 (IID8) Alexa Fluor® 594

sc-53010 AF594
200 µg/ml
RMB2685.00

SERCA2 抗体 (IID8) Alexa Fluor® 647

sc-53010 AF647
200 µg/ml
RMB2685.00

SERCA2 抗体 (IID8) Alexa Fluor® 680

sc-53010 AF680
200 µg/ml
RMB2685.00

SERCA2 抗体 (IID8) Alexa Fluor® 790

sc-53010 AF790
200 µg/ml
RMB2685.00

I am using SERCA2 (IID8): sc-53010 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: TinTin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Superb signal on human skeletal muscle1:200 dilution on a cross-section from a human skeletal muscle biopsy that had been fixed in 4% paraformaldehyde for 3hr and cryoprotected with 30% sucrose.
Date published: 2021-10-01
Rated 2 out of 5 by from Weak band at 100 kDaIn mouse skeletal muscle, this antibody resulted in a weak band at 100 kDa, but instead a clear bands appeared at 50 kDa. Will recommend SERCA2 F-1 (for muscle) instead.
Date published: 2018-01-11
Rated 4 out of 5 by from good antibodyI bought this antibody 1 month ago. I used for WB and It is good.
Date published: 2017-05-13
Rated 4 out of 5 by from Positive detection of endogenously expressedPositive detection of endogenously expressed SERCA2 protein by WB using 293T, T98G and MDCK whole cell lysate. -SCBT QC
Date published: 2015-06-27
Rated 4 out of 5 by from Produced clear immunoperoxidase cytoplasmicProduced clear immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human skeletal muscle tissue. -SCBT QC
Date published: 2015-05-06
Rated 5 out of 5 by from Published data obtained by IP using humanPublished data obtained by IP using human platelets and HEK 705 cells. -SCBT Publication Review
Date published: 2015-01-23
Rated 5 out of 5 by from Various references support the use of thisVarious references support the use of this antibody in WB using human coronary artery smooth muscle cells, platelets, HEK 705 and Jurkat cells. -SCBT QC
Date published: 2015-01-06
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SERCA2 抗体 (IID8) is rated 4.1 out of 5 by 7.
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