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Scrib Antibody (D-2) - Western Blotting - Image 377025
Scrib 抗体 (D-2): sc-374139. Hep G2 (A), JAR (B), PC-3 (C), A2058 (D) 和 T-47D (E) 全细胞裂解液中 Scrib 表达的 Western 印迹分析.
Scrib 抗体 (D-2): sc-374139. Hep G2 (A), JAR (B), PC-3 (C), A2058 (D) 和 T-47D (E) 全细胞裂解液中 Scrib 表达的 Western 印迹分析.

Scrib 抗体 (D-2): sc-374139

4.0(6)
寫評論提問

说明书
  • Scrib抗体(D-2)是小鼠单克隆IgG2a κ, 在6篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Scrib的C-terminus附近的氨基酸1489-1525之间
  • 抗-Scrib 抗体 (D-2) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Scrib
  • 抗Scrib抗体（D-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Scrib (D-2): sc-374139的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2a BP-HRP">m-IgG2a BP-HRP是Scrib Antibody (D-2) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Scrib Antibody (D-2) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Scrib抗体（D-2）是一种IgG2a κ小鼠单克隆Scrib抗体，可通过WB、IP、IF和ELISA技术检测小鼠、大鼠和人源的Scrib蛋白。Scrib抗体（D-2）既可作为非偶联抗Scrib抗体形式提供，也可作为多种偶联形式的抗Scrib抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。黑腹果蝇的基因根据其编码的蛋白质类型可分为六大类，包括细胞内信号蛋白、跨膜蛋白、RNA结合蛋白、分泌因子、转录调控因子（基本螺旋-环-螺旋、含同源域、含锌指和染色质相关）以及其他功能蛋白。果蝇的形态发生和细胞分化需要精确控制细胞分裂。大盘状瘤（Dlg）、Scribble（Scrib）和致死性巨幼细胞（Lgl）肿瘤抑制蛋白调控神经母细胞不对称细胞分裂的多个方面。Dlg/Scrib/Lgl蛋白在前期/中期显示顶端皮质富集，并具有均匀的皮质分布。编码多PDZ（PSD-95、Discs-large和ZO-1）和富含亮氨酸重复蛋白Scrib的基因突变导致细胞形态异常和胚胎上皮单层组织的丧失。人类同源物hScrib定位于脊椎动物紧密连接处，其功能是正确放置粘附连接。Scrib的PDZ结构域被预测与蛋白质C末端的共识S/TXV结合。PDZ结构域蛋白已涉及蛋白质运输途径的几个不同位点，这表明Scrib是几种上皮决定因素定位所必需的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Scrib 抗体 (D-2)

sc-374139
200 µg/ml
RMB2377.00

Scrib (D-2): m-IgG Fc BP-HRP 套装

sc-525938
200 µg Ab; 10 µg BP
RMB2662.00

Scrib (D-2): m-IgG2a BP-HRP 套装

sc-546465
200 µg Ab; 10 µg BP
RMB2662.00

Scrib 抗体 (D-2) AC

sc-374139 AC
500 µg/ml, 25% agarose
RMB3129.00

Scrib 抗体 (D-2) HRP

sc-374139 HRP
200 µg/ml
RMB2377.00

Scrib 抗体 (D-2) FITC

sc-374139 FITC
200 µg/ml
RMB2482.00

Scrib 抗体 (D-2) PE

sc-374139 PE
200 µg/ml
RMB2580.00

Scrib 抗体 (D-2) Alexa Fluor® 488

sc-374139 AF488
200 µg/ml
RMB2685.00

Scrib 抗体 (D-2) Alexa Fluor® 546

sc-374139 AF546
200 µg/ml
RMB2685.00

Scrib 抗体 (D-2) Alexa Fluor® 594

sc-374139 AF594
200 µg/ml
RMB2685.00

Scrib 抗体 (D-2) Alexa Fluor® 647

sc-374139 AF647
200 µg/ml
RMB2685.00

Scrib 抗体 (D-2) Alexa Fluor® 680

sc-374139 AF680
200 µg/ml
RMB2685.00

Scrib 抗体 (D-2) Alexa Fluor® 790

sc-374139 AF790
200 µg/ml
RMB2685.00

Scrib (D-2) 中和勝肽

sc-374139 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-374139 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from WB for mouse brain was worked wellWB for mouse brain was worked well. Signal intensity was strong.
Date published: 2017-12-09
Rated 1 out of 5 by from no detection in mouse cellsI tested free sample on thymic epithelial origin cell lines and got no specific band, but there were non-specific bands instead (around 75 and 47 kda).
Date published: 2017-11-14
Rated 5 out of 5 by from It is great.I have tried several samples of this antibody, and this might be the best
Date published: 2017-11-08
Rated 3 out of 5 by from WB worksWB only works in human tissue, doesn't work in mouse tissue
Date published: 2017-08-23
Rated 5 out of 5 by from Produced positive Western Blot data of ScribProduced positive Western Blot data of Scrib expression in HeLa whole cell lysate. -SCBT QC
Date published: 2015-04-18
Rated 5 out of 5 by from Great Western Blot data of Scrib expressionGreat Western Blot data of Scrib expression in Jurkat whole cell lysate. -SCBT QC
Date published: 2013-03-11
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Scrib 抗体 (D-2) is rated 4.0 out of 5 by 6.
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