Date published: 2025-9-8
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Scrib Antibody (C-6) - Western Blotting - Image 361998
Scrib 抗体 (C-6): sc-55543. HeLa (A) 和 MCF7 (B) 全细胞裂解液中 Scrib 表达的 Western 印迹分析.
Scrib 抗体 (C-6): sc-55543. HeLa (A) 和 MCF7 (B) 全细胞裂解液中 Scrib 表达的 Western 印迹分析.

Scrib 抗体 (C-6): sc-55543

3.1(8)
寫評論提問

说明书
  • Scrib 抗体 C-6 是小鼠单克隆 IgG2b κ，Scrib抗体, 在11篇文献中引用，规格为200 µg/ml
  • 与氨基酸1331-1630 deletion 1444-1504（缺失1444-1504）的起源在Scrib的human的C-terminus
  • Scrib 抗体 (C-6) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 Scrib
  • 抗Scrib抗体（C-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Scrib (C-6): sc-55543的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2b BP-HRP 是 Scrib 抗体 (C-6) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Scrib 抗体 (C-6) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Scrib 抗体（C-6）是一种小鼠单克隆 IgG2b kappa 轻链抗体，可通过免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附试验（ELISA）检测人源 Scrib 蛋白。抗 Scrib 抗体 (C-6) 有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。Scrib 在维持上皮细胞完整性和调节细胞极性方面起着至关重要的作用，而细胞极性对正常的组织结构和功能至关重要。Scrib (C-6) 单克隆抗体定位在脊椎动物细胞的紧密连接处，Scrib 在那里与其他蛋白相互作用，确保粘连接头的正确位置，从而影响细胞粘附和信号传导途径。这种定位对形成功能性上皮屏障至关重要，上皮屏障可保护组织免受病原体侵害并调节分子通道。Scrib 的 PDZ 结构域与目标蛋白 C 端的特定序列结合，促进关键上皮决定因子的定位，并有助于整体细胞组织。Scrib 基因突变可导致严重的发育缺陷并导致肿瘤发生，这凸显了 Scrib 在正常生理和疾病状态中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Scrib 抗体 (C-6) 参考文献:

  1. 基底侧 PDZ 蛋白 Scribble 对顶端上皮决定因子的定位。  |  Bilder, D. and Perrimon, N. 2000. Nature. 403: 676-80. PMID: 10688207
  2. 黑腹果蝇的基因组序列。  |  Adams, MD., et al. 2000. Science. 287: 2185-95. PMID: 10731132
  3. PDZ 蛋白 Scribble 对突触可塑性和突触小泡动力学的调控。  |  Roche, JP., et al. 2002. J Neurosci. 22: 6471-9. PMID: 12151526
  4. Dlg, Scrib和Lgl调节神经母细胞的大小和有丝分裂纺锤体的不对称性。  |  Albertson, R. and Doe, CQ. 2003. Nat Cell Biol. 5: 166-70. PMID: 12545176
  5. 果蝇胚胎新形成性腺中 scribble 表达的要求。  |  Marhold, J., et al. 2003. Gene Expr Patterns. 3: 143-6. PMID: 12711540
  6. 星形胶质细胞迁移过程中，Scrib控制Cdc42定位和活性以促进细胞极化。  |  Osmani, N., et al. 2006. Curr Biol. 16: 2395-405. PMID: 17081755
  7. 拉线：果蝇发育过程中的细胞周期调控。  |  Lehner, CF. 1991. Semin Cell Biol. 2: 223-31. PMID: 1842341
  8. 极性蛋白Scrib对于定向内皮细胞迁移至关重要。  |  Michaelis, UR., et al. 2013. Circ Res. 112: 924-34. PMID: 23362312
  9. 心室心肌的形态发生需要 Scrib:Rac1 的相互作用。  |  Boczonadi, V., et al. 2014. Cardiovasc Res. 104: 103-15. PMID: 25139745
  10. 不同的 PDZ 结构域识别独特的羧基末端基团。  |  Songyang, Z., et al. 1997. Science. 275: 73-7. PMID: 8974395
  11. 紧密连接  |  Balda, MS. and Matter, K. 1998. J Cell Sci. 111 (Pt 5): 541-7. PMID: 9454728
  12. RhoA 和 Rac1 小 GTP 酶对紧密连接的结构和功能调控。  |  Jou, TS., et al. 1998. J Cell Biol. 142: 101-15. PMID: 9660866

订购信息

产品名称产品编号规格价格数量收藏夹

Scrib 抗体 (C-6)

sc-55543
200 µg/ml
RMB2377.00

Scrib (C-6): m-IgG Fc BP-HRP 套装

sc-525636
200 µg Ab; 10 µg BP
RMB2662.00

Scrib (C-6): m-IgG2b BP-HRP 套装

sc-549601
200 µg Ab; 10 µg BP
RMB2662.00

Scrib 抗体 (C-6) AC

sc-55543 AC
500 µg/ml, 25% agarose
RMB3129.00

Scrib 抗体 (C-6) HRP

sc-55543 HRP
200 µg/ml
RMB2377.00

Scrib 抗体 (C-6) FITC

sc-55543 FITC
200 µg/ml
RMB2482.00

Scrib 抗体 (C-6) PE

sc-55543 PE
200 µg/ml
RMB2580.00

Scrib 抗体 (C-6) Alexa Fluor® 488

sc-55543 AF488
200 µg/ml
RMB2685.00

Scrib 抗体 (C-6) Alexa Fluor® 546

sc-55543 AF546
200 µg/ml
RMB2685.00

Scrib 抗体 (C-6) Alexa Fluor® 594

sc-55543 AF594
200 µg/ml
RMB2685.00

Scrib 抗体 (C-6) Alexa Fluor® 647

sc-55543 AF647
200 µg/ml
RMB2685.00

Scrib 抗体 (C-6) Alexa Fluor® 680

sc-55543 AF680
200 µg/ml
RMB2685.00

Scrib 抗体 (C-6) Alexa Fluor® 790

sc-55543 AF790
200 µg/ml
RMB2685.00

We are working on gastric cancer and we would like to evaluate scrib membrane labeling. What type of human tissue can be used as a witness ? Thank you very much.

Asked by: marion jost
Thank you for your question. We recommend Hela cells, MCF-7 cells and T-47D cells as positive controls for the Scrib protein.
Answered by: Tech Service
Date published: 2019-06-20

Can Scrib (C-6): sc-55543 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Scrib (C-6): sc-55543 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Didn't work in mouse IHC(Fr) or WBWe tried this antibody on sections of mouse embryo and on westerns of mouse embryo lysates, and got no specific signal.
Date published: 2019-06-03
Rated 1 out of 5 by from Not Good for IFIn Hek293, we did not detect any specific Immunofluorescence signal. Tried twice!
Date published: 2017-11-29
Rated 3 out of 5 by from WB worksWB only works in human tissue, doesn't work in mouse tissue
Date published: 2017-08-23
Rated 5 out of 5 by from Nice for western blotWorks well in 293A cells, and I got a clean band when incubated the membrane with 1:1000 dilution of primary antibody at 4°C overnight
Date published: 2017-07-25
Rated 1 out of 5 by from Didn't workSome non-specific bands at lower molecular weights, but nothing at the proper molecular weight when used in a western on NRVMs.
Date published: 2017-06-16
Rated 4 out of 5 by from Robust bands seen in WB using HeLa and JurkatRobust bands seen in WB using HeLa and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-06-30
Rated 5 out of 5 by from WB publishable data with human Jurkat T cellsWB publishable data with human Jurkat T cells - SCBT Publication Review
Date published: 2015-02-25
Rated 5 out of 5 by from Produced great immunoperoxidase cytoplasmicProduced great immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human skin tissue. -SCBT QC
Date published: 2013-12-03
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Scrib抗体 (C-6) is rated 3.1 out of 5 by 8.
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