Date published: 2025-9-8
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RUNX2 Antibody (F-2) - Western Blotting - Image 380246
RUNX2 抗体 (F-2): sc-390351. NIH/3T3 (A), c4 (B), AT3B-1 (C) 和 PC-3 (D) 全细胞裂解液中 RUNX2 表达的 Western 印迹分析.
RUNX2 抗体 (F-2): sc-390351. NIH/3T3 (A), c4 (B), AT3B-1 (C) 和 PC-3 (D) 全细胞裂解液中 RUNX2 表达的 Western 印迹分析.

RUNX2 抗体 (F-2): sc-390351

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说明书
  • RUNX2 抗体 F-2 是小鼠单克隆 IgG1 κ，RUNX2抗体, 在141篇文献中引用，规格为200 µg/ml
  • 免疫mouse物种的RUNX2的氨基酸294-363
  • RUNX2 抗体 (F-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 RUNX2; 也和以下物种反应，包括： 和 equine, bovine and porcine
  • 抗RUNX2抗体（F-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-390351 X, 200 µg/0.1 ml)
  • 关于如何获取RUNX2 (F-2): sc-390351的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 是 RUNX2 抗体 (F-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与RUNX2 抗体 (F-2) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 RUNX 抗体 包括 RUNX1, RUNX2和RUNX3

RUNX2抗体（F-2）是一种IgG1 κ小鼠单克隆RUNX2抗体（也称为CBFA1抗体、AML3抗体或PEBP2A抗体），它通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人类来源的RUNX2蛋白。RUNX2抗体（F-2）既有非偶联形式的抗RUNX2抗体，也有多种偶联形式的抗RUNX2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。哺乳动物的Runt相关转录因子（RUNX）家族包括三个成员：RUNX1（也称为AML-1、PEBP2αB、CBFA2）、RUNX2（也称为AML-3、PEBP2αA、CBFA1、Osf2）和RUNX3（也称为AML-2、PEBP2αC、CBFA3）。RUNX家族成员是结合DNA的蛋白质，可调节涉及细胞分化和细胞周期进程的基因的表达。RUNX2对于骨骼矿化至关重要，因为它能刺激间充质干细胞的成骨分化，促进软骨细胞肥大，并有助于内皮细胞迁移和发育中骨骼的血管侵袭。调节RUNX2的表达可能成为促进骨形成的有用治疗工具。RUNX2的C末端突变与锁骨颅骨发育不良综合征有关，这是一种常染色体显性骨骼发育不良综合征，其特征是颅缝广泛开放、锁骨发育不全、多生牙和身材矮小。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RUNX2 抗体 (F-2) 参考文献:

  1. RUNX2 在乳腺癌发生中的作用。  |  Wysokinski, D., et al. 2015. Int J Mol Sci. 16: 20969-93. PMID: 26404249
  2. 颅骨发育不良和RUNX2-临床表型-基因型的关联。  |  Jaruga, A., et al. 2016. Clin Genet. 90: 393-402. PMID: 27272193
  3. 成骨细胞分化中microRNA对Runx2的调控。  |  Narayanan, A., et al. 2019. Life Sci. 232: 116676. PMID: 31340165
  4. Runx2依赖性骨骼发育的分子机制。  |  Komori, T. 2020. Mol Cells. 43: 168-175. PMID: 31896233
  5. RUNX2通过BMP4信号通路调节成骨细胞分化。  |  Liu, DD., et al. 2022. J Dent Res. 101: 1227-1237. PMID: 35619284
  6. Runx2调节染色质可及性，以指导新生儿阶段的成骨细胞程序。  |  Hojo, H., et al. 2022. Cell Rep. 40: 111315. PMID: 36070691
  7. Runx2和Runx3在手术诱导的骨关节炎发展过程中对关节软骨细胞进行差异性调节。  |  Nagata, K., et al. 2022. Nat Commun. 13: 6187. PMID: 36261443
  8. 4-羟基-2-壬烯醛对Runx2 K176的羰基化加速血管钙化。  |  Zhai, X., et al. 2024. Circulation. 149: 1752-1769. PMID: 38348663
  9. FOXO1通过SMURF2调节钙化主动脉瓣膜疾病中RUNX2的泛素化。  |  Jiang, C., et al. 2024. Redox Biol. 73: 103215. PMID: 38810422
  10. RUNX2作为人类癌症的预后因子。  |  Toner, J., et al. 2024. Crit Rev Eukaryot Gene Expr. 34: 51-66. PMID: 39072409

订购信息

产品名称产品编号规格价格数量收藏夹

RUNX2 抗体 (F-2)

sc-390351
200 µg/ml
RMB2377.00

RUNX2 (F-2): m-IgG Fc BP-HRP 套装

sc-526149
200 µg Ab; 10 µg BP
RMB2662.00

RUNX2 抗体 (F-2) X

sc-390351 X
200 µg/0.1 ml
RMB2377.00

RUNX2 抗体 (F-2) AC

sc-390351 AC
500 µg/ml, 25% agarose
RMB3129.00

RUNX2 抗体 (F-2) HRP

sc-390351 HRP
200 µg/ml
RMB2377.00

RUNX2 抗体 (F-2) FITC

sc-390351 FITC
200 µg/ml
RMB2482.00

RUNX2 抗体 (F-2) PE

sc-390351 PE
200 µg/ml
RMB2580.00

RUNX2 抗体 (F-2) Alexa Fluor® 488

sc-390351 AF488
200 µg/ml
RMB2685.00

RUNX2 抗体 (F-2) Alexa Fluor® 546

sc-390351 AF546
200 µg/ml
RMB2685.00

RUNX2 抗体 (F-2) Alexa Fluor® 594

sc-390351 AF594
200 µg/ml
RMB2685.00

RUNX2 抗体 (F-2) Alexa Fluor® 647

sc-390351 AF647
200 µg/ml
RMB2685.00

RUNX2 抗体 (F-2) Alexa Fluor® 680

sc-390351 AF680
200 µg/ml
RMB2685.00

RUNX2 抗体 (F-2) Alexa Fluor® 790

sc-390351 AF790
200 µg/ml
RMB2685.00

Do you have a RUNX2 antibody that reacts with sheep bone in IHC-P

Asked by: Liska
Thank you for your question. Anti-RUNX2 Antibody (C-12) sc-390715 is recommended for detection of RUNX2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, bovine, porcine and canine. RUNX2 Antibody (F-2) sc-390351 is recommended for detection of RUNX2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA; also reactive with additional species, including and equine, bovine and porcine. Neither of these antibodies has been tested on sheep cell or tissue samples so we cannot say whether or not it would also be reactive in such a setup.
Answered by: Tech Support Europe
Date published: 2024-03-28

does RUNX2 Antibody (F-2): sc-390351 antibody work in tissue from dogs and cats?

Asked by: Rphtorres
Thank you for your question. We would not recommend this antibody for dog or cat samples . There is a 68%homology in dog and 45% homology in cat.
Answered by: BlakeJ
Date published: 2023-07-11

This antibody works very well in IF when I use anti-mouse IGG or IGG1, but it doesn't when I use IGG2b. Are you sure it is IGG2b? I don't think so. Thanks

Asked by: chicobotelho
Thank you for your question. Also, thank you for noticing that we had the wrong isotype for this antibody, we had missed that error. It has since been fixed!
Answered by: Technical Support
Date published: 2022-06-10

what about the price of this antibody?

Asked by: miniminxi
For accurate availability, pricing and shipping information regarding this product, please contact customer service.
Answered by: Tech Service
Date published: 2019-01-04

I have Runx2 ab tag with fluor 488, my question is can I use the ab for western blot detection.

Asked by: amira raudhah
Thank you for your question. Yes, this antibody is fine for WB, and the antibody conjugated with AF488 is fine for use in WB as long as you have the capability to detect that wavelength signal. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-11-28

Can this antibody be used for IHC or IF on mouse tissue samples?

Asked by: Anna99
You can stain mouse tissue by using a directly conjugated primary antibody. RUNX2 Antibody (F-2) is available conjugated to HRP (sc-390351 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-390351 PE), fluorescein (sc-390351 FITC), Alexa Fluor® 488 (sc-390351 AF488) or Alexa Fluor® 647 (sc-390351 AF647), 200 µg/ml, for IF, IHC(P) and FCM.
Answered by: Technical Support 6
Date published: 2017-01-11

What is the best secondary to use with this mouse primary antibody for WB?

Asked by: Anna99
We recommend using our Mouse IgG kappa Binding Protein HRP, sc-516102. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 6
Date published: 2017-01-11
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Rated 5 out of 5 by from Great results on canine osteosarcomaWe tested the antibody with a 1:150 concentration. It works after HIER.
Date published: 2023-01-19
Rated 5 out of 5 by from Very good results in WB and IFWorks very good in Western Blot and immunofluorescence (4% PFA fixation). Tested on mouse cells.
Date published: 2021-06-25
Rated 5 out of 5 by from RUNX2 (F-2): sc-390351An excellent result was obtained in Western blotting and immunofluorescence in bone marrow stem cells (BMSC)
Date published: 2021-06-15
Rated 5 out of 5 by from RunX2 (F-2) antibodyRunX2 (F-2) antibody worked nicely with animal heart samples at a 1:200 dilution.
Date published: 2018-03-29
Rated 4 out of 5 by from 比较适合WB做Western blot结果还可以。IHC我主要用来检测osteoblast中的Runx2表达，在幼龄小鼠（1week以下）组织中结果比较好，年龄较大的小鼠会出现一些非特异性信号。
Date published: 2017-12-18
Rated 5 out of 5 by from Good antibody for WBI used 1:300 dilution of the antibody on osseous tissue overnight at 4 degrees.
Date published: 2017-10-24
Rated 5 out of 5 by from The antibody is very niceRUNX2 Antibody worked well in the mouse, the background is transparent and simple operation, which has been more applications.
Date published: 2017-10-16
Rated 3 out of 5 by from Canine SamplesThis antibody work fine with canine OS cell lines at 1:500
Date published: 2017-10-10
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RUNX2 抗体 (F-2) is rated 4.6 out of 5 by 28.
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