Date published: 2025-9-8
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RIP Antibody (C-12) - Western Blotting - Image 375343
RIP 抗体 (C-12): sc-133102. HeLa (A) 和 T24 (B) 全细胞裂解液中 RIP 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
RIP 抗体 (C-12): sc-133102. HeLa (A) 和 T24 (B) 全细胞裂解液中 RIP 表达的近红外 Western 印迹分析. 用 UltraCruz® 阻断试剂阻断: sc-516214. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

RIP 抗体 (C-12): sc-133102

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寫評論提問

说明书
  • RIP 抗体 C-12 是小鼠单克隆 IgG1 κ，RIP抗体, 在32篇文献中引用，规格为200 µg/ml
  • 针对氨基酸465-671，在RIP (receptor interacting protein)的human起源的C-terminus处进行映射
  • RIP 抗体 (C-12) 推荐用于 WB, IP, IF 和 ELISA，检测mouse 和human 来源的 RIP
  • 抗RIP抗体（C-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取RIP (C-12): sc-133102的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是RIP Antibody (C-12) for WB applications. 的首选辅助检测试剂，这些试剂现与RIP Antibody (C-12) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

附加RIP抗体，包括RIP140, RIP3, RIP4和RIP5

RIP抗体（C-12）是一种IgG1 κ小鼠单克隆RIP抗体，可通过WB, IP, IF和ELISA检测小鼠和人类来源的RIP蛋白。RIP抗体（C-12）既有非偶联形式的抗RIP抗体，也有多种偶联形式的抗RIP抗体，包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种Alexa Fluor®偶联物。与促进细胞增殖的生长因子不同，FAS配体（FAS-L）和肿瘤坏死因子（TNFs）可迅速诱导细胞凋亡。细胞对FAS-L和TNF的反应是由结构上相关的受体介导的，这些受体包含一个保守的"死亡域"，属于TNF受体超家族。TRADD、FADD和RIP是含有死亡域同源区（DDH）的FAS/TNF-R1相互作用蛋白。TRADD（TNF-R1相关死亡域）和FADD（FAS相关死亡域）通过其DDH区与FAS和TNF-R1的死亡域结合。TRADD的过度表达可在无TNF的情况下激活NFκB并诱导细胞凋亡。FADD的过度表达可引起细胞凋亡，而牛痘蛋白CrmA可阻断这一过程，这表明FADD位于ICE和其他可能的丝氨酸蛋白酶的上游。受体相互作用蛋白RIP仅通过其DDH与FAS结合，而这种结合在lpr突变体中被废除。与TRADD和FADD不同，RIP包含一个假定的氨基末端激酶域。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

RIP 抗体 (C-12) 参考文献:

  1. 肝细胞生长因子（HGF）在大鼠出生后的发育过程中促进少突胶质祖细胞增殖并抑制其分化。  |  Ohya, W., et al. 2007. Brain Res. 1147: 51-65. PMID: 17382307
  2. 受体相互作用蛋白激酶-3决定细胞对TNF-α的坏死反应。  |  He, S., et al. 2009. Cell. 137: 1100-11. PMID: 19524512
  3. 抑制受体相互作用蛋白激酶 1 可改善实验性非酒精性脂肪肝。  |  Majdi, A., et al. 2020. J Hepatol. 72: 627-635. PMID: 31760070
  4. Quizartinib通过靶向受体相互作用丝氨酸/苏氨酸蛋白激酶1抑制坏死。  |  Li, M., et al. 2023. FASEB J. 37: e23178. PMID: 37698367
  5. 法斯死亡因素  |  Nagata, S. and Golstein, P. 1995. Science. 267: 1449-56. PMID: 7533326
  6. FAP-1：一种与 Fas 相关联的蛋白酪氨酸磷酸酶。  |  Sato, T., et al. 1995. Science. 268: 411-5. PMID: 7536343
  7. FADD是一种新型含死亡结构域的蛋白质，它与Fas的死亡结构域相互作用，启动细胞凋亡。  |  Chinnaiyan, AM., et al. 1995. Cell. 81: 505-12. PMID: 7538907
  8. RIP：一种含有死亡结构域的新型蛋白质，在酵母中与 Fas/APO-1 (CD95) 相互作用并导致细胞死亡。  |  Stanger, BZ., et al. 1995. Cell. 81: 513-23. PMID: 7538908
  9. FasL/TNF 死亡信号的竞争者。  |  Cleveland, JL. and Ihle, JN. 1995. Cell. 81: 479-82. PMID: 7758103
  10. TNF 受体 1 相关蛋白 TRADD 发出细胞死亡和 NF-kappa B 激活信号。  |  Hsu, H., et al. 1995. Cell. 81: 495-504. PMID: 7758105
  11. 细胞和病毒蛋白 TNF 受体超家族：激活, 成本刺激和死亡。  |  Smith, CA., et al. 1994. Cell. 76: 959-62. PMID: 8137429
  12. 生与死的转换器TNF 受体超家族及相关蛋白。  |  Baker, SJ. and Reddy, EP. 1996. Oncogene. 12: 1-9. PMID: 8552378

订购信息

产品名称产品编号规格价格数量收藏夹

RIP 抗体 (C-12)

sc-133102
200 µg/ml
RMB2377.00

RIP (C-12): m-IgG Fc BP-HRP 套装

sc-527092
200 µg Ab; 10 µg BP
RMB2662.00

RIP (C-12): m-IgG1 BP-HRP 套装

sc-532465
200 µg Ab; 20 µg BP
RMB2662.00

RIP 抗体 (C-12) AC

sc-133102 AC
500 µg/ml, 25% agarose
RMB3129.00

RIP 抗体 (C-12) HRP

sc-133102 HRP
200 µg/ml
RMB2377.00

RIP 抗体 (C-12) FITC

sc-133102 FITC
200 µg/ml
RMB2482.00

RIP 抗体 (C-12) PE

sc-133102 PE
200 µg/ml
RMB2580.00

RIP 抗体 (C-12) Alexa Fluor® 488

sc-133102 AF488
200 µg/ml
RMB2685.00

RIP 抗体 (C-12) Alexa Fluor® 546

sc-133102 AF546
200 µg/ml
RMB2685.00

RIP 抗体 (C-12) Alexa Fluor® 594

sc-133102 AF594
200 µg/ml
RMB2685.00

RIP 抗体 (C-12) Alexa Fluor® 647

sc-133102 AF647
200 µg/ml
RMB2685.00

RIP 抗体 (C-12) Alexa Fluor® 680

sc-133102 AF680
200 µg/ml
RMB2685.00

RIP 抗体 (C-12) Alexa Fluor® 790

sc-133102 AF790
200 µg/ml
RMB2685.00

RIP和RIP3抗体都能在55kD处显示一条极其明显的条带，请问这是什么情况？

Asked by: 胡萝卜糊了
感谢您的提问。很抱歉该产品的实验效果未能达到您的预期。我们希望您在研究上取得成功，请通过电话、电子邮件，或者在线聊天联系我们的技术服务部门。 Thank you for your question. We are sorry to hear that this product did not perform as expected. We want you to be successful in your research, please contact our Technical Service Department for support by phone, e-mail or live chat.
Answered by: Jiajun Z
Date published: 2023-07-13

RIP Antibody (C-12): sc-133102 Does this antibody also detect other RIP family proteins? Such as RIP3, RIP4,,,,,,, Thanks

Asked by: UW Madison
sc-133102 has not been validated to work in RIP3 and RIP 4. Here is a link for more information on this antibody . https://www.uniprot.org/uniprotkb/Q13546/entry#sequences
Answered by: BlakeJ
Date published: 2023-05-31

RIP 抗体 (C-12): sc-133102   Can this product be used in rats?Do your company have a suitable RIP1 Primary antibodies inrats?

Asked by: chenyg
Thank you for your question. The immunogen used for this RIP antibody, amino acids 465-671 of RIP of human origin, does not show significant homology with the rat sequence for RIP. At this time this is the only RIP antibody available, so we do not have one to recommend for detection of RIP in rats.
Answered by: Technical Service
Date published: 2017-04-16

Can you recommend a positive control to use with RIP for Western blot?

Asked by: cjMara
We recommend 3T3-L1: sc-2243, K-562: sc-2203 or MOLT-4: sc-2233 whole cell lysates to use as positive controls as we have great Western blot data showing specific detection of RIP in these cell lines.
Answered by: Technical Support 8
Date published: 2017-01-12

What is the recommended secondary reagent to use with sc-133102?

Asked by: cjMara
Thank you for your question. While most anti-mouse IgG secondary antibodies are appropriate for use with our RIP (C-12): sc-133102, we recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 8
Date published: 2017-01-12
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Rated 5 out of 5 by from Good antibodyThe antibody is good, it is at the expected size on the WB. I like it.
Date published: 2020-01-28
Rated 4 out of 5 by from Expected resultsWe have obtained expected band by using antibody on western Blot, both in tissues of mouse major organs and culture cells, although expression levels varied among them, based on ECl staining.
Date published: 2019-03-12
Rated 5 out of 5 by from WB AND IP5% BSA BLOCKED, AND THE BAND IS CLEAN ,THE RESULTS OF IP AND CO-IP ARE WORKED.
Date published: 2017-12-12
Rated 4 out of 5 by from Works with overexpressed RIP1Works with human RIP1 overexpressed in 293T cells.
Date published: 2017-10-16
Rated 4 out of 5 by from Good antibodyThis antibody works well for western blot and immunoprecipitation
Date published: 2017-10-10
Rated 3 out of 5 by from Not good enough.We've got weak signal when detecting mouse brain lysates at even 1:200 dilution. However, it might owe to the low expression in brain tissue.
Date published: 2017-09-09
Rated 5 out of 5 by from good for WbThis antibody works well when we use 1:500 dilution in WB, and our results shows a beautiful brand.
Date published: 2017-08-23
Rated 5 out of 5 by from good antibodyI am statisfied with the antibody and it is very good
Date published: 2017-07-25
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RIP 抗体 (C-12) is rated 4.7 out of 5 by 23.
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