Date published: 2025-9-7

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PHF21A 抗体 (F-9): sc-376844

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说明书
  • PHF21A 抗体 F-9 是小鼠单克隆 IgG2b (kappa轻链),PHF21A抗体,规格为200 µg/ml
  • 免疫原氨基酸序列571-675位于human物种的PHF21A的C-terminus
  • PHF21A 抗体 (F-9) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 PHF21A
  • 抗PHF21A抗体(F-9)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PHF21A (F-9): sc-376844免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRPm-IgGκ BP-HRP 是 PHF21A 抗体 (F-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PHF21A 抗体 (F-9) 组合成套装提供。 (请参阅下面的订购信息)。

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    関連項目

    PHF21A抗体(F-9)是一种IgG2b κ小鼠单克隆PHF21A抗体(也称为PHF21A抗体),可通过WB、IP、IF、IHC(P)和ELISA检测人类来源的PHF21A蛋白。PHF21A抗体(F-9)既可以是非偶联的抗PHF21A抗体形式,也可以是多种偶联形式的抗PHF21A抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。锌指蛋白含有DNA结合域,具有多种功能,其中大多数包括某种形式的转录激活或抑制。PHF21A(PHD指蛋白21A),也称为BRAF35-HDAC复合蛋白BHC80,是一种含有一个PHD型锌指和一个A.T钩DNA结合域的680个氨基酸的核蛋白,暗示其参与转录调控事件。PHF21A是BHC复合物的组成部分,负责抑制非神经元细胞中神经元特异性基因的转录。BHC复合物作为染色质修饰剂,对组蛋白上的特定位点进行去乙酰化和去甲基化。PHF21A可能在BHC复合物中起支架作用。PHF21A主要在大脑中表达,由于选择性剪接事件,存在三种PHF21A异构体。

    仅限研究使用。不适用于诊断和治疗用途。

    Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

    LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

    PHF21A 抗体 (F-9) 参考文献:

    1. 含有组蛋白去乙酰化酶的核心-BRAF35复合物介导了对神经元特异性基因的抑制。  |  Hakimi, MA., et al. 2002. Proc Natl Acad Sci U S A. 99: 7420-5. PMID: 12032298
    2. 一个候选的 X 连锁智力迟钝基因是一个新的含组蛋白去乙酰化酶复合物家族的组成部分。  |  Hakimi, MA., et al. 2003. J Biol Chem. 278: 7234-9. PMID: 12493763
    3. BRAF-HDAC 复合物中参与神经元特异性基因抑制的 BHC80 的特征。  |  Iwase, S., et al. 2004. Biochem Biophys Res Commun. 322: 601-8. PMID: 15325272
    4. 相关因子对 LSD1 组蛋白去甲基化酶活性的调控  |  Shi, YJ., et al. 2005. Mol Cell. 19: 857-64. PMID: 16140033
    5. 类 CoREST 复合物调控染色质修饰和神经元基因表达。  |  Lakowski, B., et al. 2006. J Mol Neurosci. 29: 227-39. PMID: 17085781
    6. 小鼠胚胎干细胞早期分化的基因功能  |  Hailesellasse Sene, K., et al. 2007. BMC Genomics. 8: 85. PMID: 17394647
    7. 识别未甲基化组蛋白 H3 赖氨酸 4 将 BHC80 与 LSD1 介导的基因抑制联系起来。  |  Lan, F., et al. 2007. Nature. 448: 718-22. PMID: 17687328
    8. Esco2 是一种新型核心抑制因子,能与多种染色质修饰酶结合。  |  Kim, BJ., et al. 2008. Biochem Biophys Res Commun. 372: 298-304. PMID: 18501190
    9. 神经分泌表型的抑制受 BHC80(一种 BRAF/HDAC 复合物蛋白)的负调控。  |  Klajn, A., et al. 2009. J Neurosci. 29: 6296-307. PMID: 19439607

    订购信息

    产品名称产品编号规格价格数量收藏夹

    PHF21A 抗体 (F-9)

    sc-376844
    200 µg/ml
    RMB2377.00

    PHF21A (F-9): m-IgGκ BP-HRP 套装

    sc-523113
    200 µg Ab, 40 µg BP
    RMB2662.00

    PHF21A (F-9): m-IgG2b BP-HRP 套装

    sc-549088
    200 µg Ab; 10 µg BP
    RMB2662.00

    PHF21A 抗体 (F-9) AC

    sc-376844 AC
    500 µg/ml, 25% agarose
    RMB3129.00

    PHF21A 抗体 (F-9) HRP

    sc-376844 HRP
    200 µg/ml
    RMB2377.00

    PHF21A 抗体 (F-9) FITC

    sc-376844 FITC
    200 µg/ml
    RMB2482.00

    PHF21A 抗体 (F-9) PE

    sc-376844 PE
    200 µg/ml
    RMB2580.00

    PHF21A 抗体 (F-9) Alexa Fluor® 488

    sc-376844 AF488
    200 µg/ml
    RMB2685.00

    PHF21A 抗体 (F-9) Alexa Fluor® 546

    sc-376844 AF546
    200 µg/ml
    RMB2685.00

    PHF21A 抗体 (F-9) Alexa Fluor® 594

    sc-376844 AF594
    200 µg/ml
    RMB2685.00

    PHF21A 抗体 (F-9) Alexa Fluor® 647

    sc-376844 AF647
    200 µg/ml
    RMB2685.00

    PHF21A 抗体 (F-9) Alexa Fluor® 680

    sc-376844 AF680
    200 µg/ml
    RMB2685.00

    PHF21A 抗体 (F-9) Alexa Fluor® 790

    sc-376844 AF790
    200 µg/ml
    RMB2685.00

    Does the Anti-PHF21A Antibody (F-9): sc-376844 react with mouse PHF21A? We would like to use for western blot analysis in mouse cells. Happy to test if you send a sample as we have mouse lines with PHF21A CRISPR'd out.

    Asked by: Gilbert1984
    Thank you for your question. Anti-PHF21A Antibody (F-9): sc-376844 is only recommended for detecting human PHF21A. If you are interested in a sample, please contact Technical Service for more details.
    Answered by: Santa Cruz TS
    Date published: 2021-07-05

    Can PHF21A(F-9): sc-376844 monoclonal antibody be used to immunofluorescence?

    Asked by: lynana
    Thank you for your question. Yes, this mouse monoclonal antibody, PHF21A (F-9): sc-376844, is recommended for detection of PHF21A of human origin by WB, IP, IF (immunofluorescence), IHC(P) and ELISA.
    Answered by: Technical Support
    Date published: 2017-07-18

    Can PHF21A (F-9): sc-376844 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

    Asked by: Dr Ninau Qelp
    Thank you for your question. Yes, PHF21A (F-9): sc-376844 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
    Answered by: Technical Support
    Date published: 2017-02-27
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    Rated 1 out of 5 by from Unsuccessful for IPUse of antibody for immunoprecipitation in HeLa cells. Consistently produces two nonspecific bands but not PHF21a.
    Date published: 2019-03-25
    Rated 5 out of 5 by from Excellent immunoperoxidase nuclear stainingExcellent immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human smooth muscle tissue. -SCBT QC
    Date published: 2015-03-29
    Rated 5 out of 5 by from Produced positive Western Blot data of PHF21AProduced positive Western Blot data of PHF21A expression in SH-SY5Y and IMR-32 nuclear extracts. -SCBT QC
    Date published: 2014-01-22
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    PHF21A 抗体 (F-9) is rated 3.7 out of 5 by 3.
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