Date published: 2025-9-6
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p-Stat1 Antibody (A-2) - Flow Cytometry - Image 5938
p-Stat1 抗体 (A-2) PE: sc-8394 PE. 固定和通透对照组 (绿线直方图) 和 IFNg 诱导组 (橙色实线直方图) U-937 细胞的胞内 FCM 分析. 粉红色虚线直方图代表同型对照, 正常小鼠 IgG1: sc-2866.
p-Stat1 Antibody (A-2) - Flow Cytometry - Image 5938
p-Stat1 抗体 (A-2) PE: sc-8394 PE. 固定和通透对照组 (绿线直方图) 和 IFNg 诱导组 (橙色实线直方图) U-937 细胞的胞内 FCM 分析. 粉红色虚线直方图代表同型对照, 正常小鼠 IgG1: sc-2866.
p-Stat1 Antibody (A-2) - Western Blotting - Image 905
未经处理 (A, C) 和γ-干扰素诱导 (B, D) 的 HeLa 细胞中 Stat1 活化的 Western 印迹分析. 测试的抗体包括磷酸化特异性 Stat1 单克隆抗体 p-Stat1 抗体 (A-2): sc-8394 (A, B) 和对照抗体 Stat1α p91 (C-111): sc-417 (C, D).
p-Stat1 抗体 (A-2) PE: sc-8394 PE. 固定和通透对照组 (绿线直方图) 和 IFNg 诱导组 (橙色实线直方图) U-937 细胞的胞内 FCM 分析. 粉红色虚线直方图代表同型对照, 正常小鼠 IgG1: sc-2866.

p-Stat1 抗体 (A-2): sc-8394

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寫評論提問

说明书
  • p-Stat1抗体(A-2)是小鼠单克隆IgG1 κ, 在107篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Stat1
  • 抗-p-Stat1 抗体 (A-2) 推荐用于 WB, IP, IF, FCM 和 ELISA，检测mouse, rat 和human 来源的 Stat1 phosphorylated at Tyr 701
  • 抗p-Stat1抗体（A-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-8394 X, 200 µg/0.1 ml)
  • 关于如何获取p-Stat1 (A-2): sc-8394的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是p-Stat1 Antibody (A-2) for WB applications. 的首选辅助检测试剂，这些试剂现与p-Stat1 Antibody (A-2) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Stat抗体，包括Stat1, Stat2, Stat3, Stat4, Stat5和Stat6

p-Stat1抗体（A-2）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、流式细胞术（FCM）和酶联免疫 酶联免疫吸附（ELISA）法。p-Stat1 (A-2) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 结合型。Stat1在介导细胞对干扰素和其他细胞因子的反应中起着至关重要的作用，它作为一种转录因子，可调节免疫反应、细胞生长和细胞凋亡相关基因的表达。Stat1在酪氨酸701处的磷酸化对于激活和随后与Stat2形成二聚体至关重要，从而形成ISGF3复合物，该复合物可转移到细胞核中，启动干扰素刺激基因的转录。这一过程对于抗病毒反应和免疫功能调节至关重要，凸显了Stat1在维持细胞稳态和对外部刺激作出反应方面的重要性。抗Stat1抗体（A-2）可准确检测磷酸化Stat1，因此p-Stat1（A-2）单克隆抗体成为研究人员研究免疫反应和细胞调节相关信号传导途径的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-Stat1 抗体 (A-2) 参考文献:

  1. STAT1 功能增益与慢性脱皮病。  |  Molho-Pessach, V., et al. 2020. Pediatr Dermatol. 37: 153-155. PMID: 31637766
  2. STAT1在卵巢癌中的双重作用： 洞察分子机制和应用潜力.  |  Li, X., et al. 2021. Front Cell Dev Biol. 9: 636595. PMID: 33834023
  3. STAT1 N 端结构域可区分控制 I 型和 II 型 IFN 信号传导。  |  Göder, A., et al. 2021. Cytokine. 144: 155552. PMID: 34000478
  4. STAT1是建立而非维持干扰素-γ诱导的转录记忆所必需的。  |  Tehrani, SS., et al. 2023. EMBO J. 42: e112259. PMID: 37272165
  5. 酪氨酸磷酸化的 Stat1 和 Stat2 加上一个 48 kDa 蛋白都与 DNA 接触，形成干扰素刺激基因因子 3。  |  Qureshi, SA., et al. 1995. Proc Natl Acad Sci U S A. 92: 3829-33. PMID: 7537377
  6. Jun 和 Stat3 的一种简称 Stat3 beta 的合作转录活性。  |  Schaefer, TS., et al. 1995. Proc Natl Acad Sci U S A. 92: 9097-101. PMID: 7568080
  7. 对多肽配体的转录反应：JAK-STAT 途径。  |  Schindler, C. and Darnell, JE. 1995. Annu Rev Biochem. 64: 621-51. PMID: 7574495
  8. 白细胞介素-3, 促红细胞生成素和催乳素可激活淋巴细胞中的 STAT5 类因子。  |  Pallard, C., et al. 1995. J Biol Chem. 270: 15942-5. PMID: 7608147
  9. Stat4，一种在早期骨髓分化过程中表达的新型γ干扰素激活位点结合蛋白。  |  Yamamoto, K., et al. 1994. Mol Cell Biol. 14: 4342-9. PMID: 8007943
  10. 白细胞介素-4 诱导的转录因子：IL-4 Stat.  |  Hou, J., et al. 1994. Science. 265: 1701-6. PMID: 8085155
  11. Stat3：一种 STAT 家族成员，在表皮生长因子和白细胞介素-6 的作用下通过酪氨酸磷酸化激活。  |  Zhong, Z., et al. 1994. Science. 264: 95-8. PMID: 8140422
  12. Jak-STAT 通路和转录激活对 IFNs 及其他细胞外信号蛋白的反应。  |  Darnell, JE., et al. 1994. Science. 264: 1415-21. PMID: 8197455

订购信息

产品名称产品编号规格价格数量收藏夹

p-Stat1 抗体 (A-2)

sc-8394
200 µg/ml
RMB2377.00

p-Stat1 (A-2): m-IgG Fc BP-HRP 套装

sc-526516
200 µg Ab; 10 µg BP
RMB2662.00

p-Stat1 (A-2): m-IgG1 BP-HRP 套装

sc-531889
200 µg Ab; 20 µg BP
RMB2662.00

p-Stat1 抗体 (A-2) X

sc-8394 X
200 µg/0.1 ml
RMB2377.00

p-Stat1 抗体 (A-2) AC

sc-8394 AC
500 µg/ml, 25% agarose
RMB3129.00

p-Stat1 抗体 (A-2) HRP

sc-8394 HRP
200 µg/ml
RMB2377.00

p-Stat1 抗体 (A-2) FITC

sc-8394 FITC
200 µg/ml
RMB2482.00

p-Stat1 抗体 (A-2) PE

sc-8394 PE
200 µg/ml
RMB2580.00

p-Stat1 抗体 (A-2) Alexa Fluor® 488

sc-8394 AF488
200 µg/ml
RMB2685.00

p-Stat1 抗体 (A-2) Alexa Fluor® 546

sc-8394 AF546
200 µg/ml
RMB2685.00

p-Stat1 抗体 (A-2) Alexa Fluor® 594

sc-8394 AF594
200 µg/ml
RMB2685.00

p-Stat1 抗体 (A-2) Alexa Fluor® 647

sc-8394 AF647
200 µg/ml
RMB2685.00

p-Stat1 抗体 (A-2) Alexa Fluor® 680

sc-8394 AF680
200 µg/ml
RMB2685.00

p-Stat1 抗体 (A-2) Alexa Fluor® 790

sc-8394 AF790
200 µg/ml
RMB2685.00

p-Stat1 (A-2) 中和勝肽

sc-8394 P
100 µg/0.5 ml
RMB511.00

你好，ND7/23细胞蛋白孵育p-STAT1抗体的条带有图片吗

Asked by: bibabo
Thank you for your question.It would be helpful if you could call us directly at (+86) 021-60936350 or email our Asia Service department at [email protected] for more interactive discussions on this and other related issues. 感谢您的提问。如果您能直接拨打(+86) 021-60936350，或者发邮件到我们亚洲服务部的邮箱[email protected]我们就这个问题和其他相关问题进行更多的互动讨论，那将会很有帮助。
Answered by: Zhanglong
Date published: 2023-11-23

Would it work of PFA fixed froyen mouse tissue in confocal microscopy? If yes, is antigen retrival necessary?

Asked by: RUNA
Thank you for your question. This antibody is not recommended for staining of tissue samples, so we cannot guarantee that it would work in your experiment.
Answered by: Tech Support Europe
Date published: 2023-09-18

Hi, Has anyone tried this antibody for IHC (P) or frozen human sections?

Asked by: IHChuman
Thank you for your question. This antibody has been published in IHC on human samples, so yes, it is both recommended and warrantied for that use. If you have any further questions, please contact our European Technical Service team. You can reach them by phone at Toll Free: +49 6221 4503 0, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-06-28

I need to use an antibody to detect the protein in samples of canine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 4
Date published: 2017-01-09
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Rated 1 out of 5 by from Detección de proteina no muy buena.Esta enticuerpo no nos ha dado buena detección de pSTA1 en celulas estrelladas.
Date published: 2019-12-14
Rated 4 out of 5 by from Good antibodyThis antibody worked very well with fixed frozen mouse tissue sections for IF
Date published: 2018-10-03
Rated 5 out of 5 by from works greatWe tried this sample on our cell line for Western, it worked great
Date published: 2018-06-22
Rated 5 out of 5 by from Works great!Gave nice bands for WB when used at 1:200 in mouse lysates.
Date published: 2018-03-22
Rated 4 out of 5 by from Good bandProbed AML cell line Kasumi-1. Got a nice clear single band.
Date published: 2018-02-22
Rated 5 out of 5 by from GreatThese are trial samples, including p-Stat3 and Stat3, but help me complete one set of experimental. These antibodies work well in HepG2 cell line.
Date published: 2018-02-14
Rated 2 out of 5 by from It can not be detected in LPS plus IFN induced RAWIt can not be detected in LPS plus IFN induced RAW
Date published: 2018-02-05
Rated 4 out of 5 by from GreatI got this antibody last month, and the STAT1 antibody can work very well, and got clearly protein band, but as for p-STAT1 antibody, not so good anyway.
Date published: 2018-01-22
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p-Stat1 抗体 (A-2) is rated 4.2 out of 5 by 26.
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