Date published: 2025-9-6

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Stat1 抗体 (C-136): sc-464

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说明书
  • Stat1 抗体 C-136 是小鼠单克隆 IgG1 κ,Stat1抗体, 在194篇文献中引用,规格为200 µg/ml
  • human Stat1 613-739的氨基酸之间的表位图谱
  • Stat1 抗体 (C-136) 推荐用于 WB, IP 和 IF,检测mouse, rat 和human 来源的 Stat1
  • 抗Stat1抗体(C-136)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-464 X, 200 µg/0.1 ml)
  • 关于如何获取Stat1 (C-136): sc-464免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP是Stat1 Antibody (C-136) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现可与Stat1 Antibody (C-136) 搭配使用(请参阅下面的订购信息)。有关其它 m-IgGκ BP 结合物,请参阅我们完整的小鼠 IgG 结合蛋白列表。

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関連項目

Stat1 抗体(C-136)是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)和免疫荧光(IF)检测小鼠、大鼠和人源的 Stat1 蛋白。Stat1 (C-136) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶、藻红蛋白、异硫氰酸荧光素和多种 Alexa Fluor® 结合物。 Stat1 蛋白在介导细胞对干扰素和其他细胞因子的反应中起着至关重要的作用,这些细胞因子对于免疫反应和基因表达调控至关重要。在受配体(如干扰素)激活后,Stat1发生酪氨酸磷酸化,从而与Stat2形成异二聚体,后者是ISGF3转录因子复合物的一部分,可转移到细胞核中启动干扰素刺激基因的转录。这一过程对于抗病毒反应和免疫功能调节至关重要,凸显了Stat1在维持细胞稳态和对外部刺激作出反应方面的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Stat1 抗体 (C-136) 参考文献:

  1. STAT1与病原体并非友好关系。  |  Najjar, I. and Fagard, R. 2010. Biochimie. 92: 425-44. PMID: 20159032
  2. Stat1是神经炎症期间神经干细胞自我更新程序的诱导性转录抑制因子。  |  Imitola, J., et al. 2023. Front Cell Neurosci. 17: 1156802. PMID: 37663126
  3. STAT3和STAT1在癌症生物学中的互补作用:对肿瘤发病机制和治疗策略的启示  |  Wang, W., et al. 2023. Front Immunol. 14: 1265818. PMID: 38022653
  4. 人类 STAT1 功能增益与慢性皮肤粘膜念珠菌病: 加强病床观察与实验室研究之间联系的综合综述》。  |  Asano, T., et al. 2024. Immunol Rev. 322: 81-97. PMID: 38084635
  5. 酪氨酸磷酸化的 Stat1 和 Stat2 加上一个 48 kDa 蛋白都与 DNA 接触,形成干扰素刺激基因因子 3。  |  Qureshi, SA., et al. 1995. Proc Natl Acad Sci U S A. 92: 3829-33. PMID: 7537377
  6. Jun 和 Stat3 的一种简称 Stat3 beta 的合作转录活性。  |  Schaefer, TS., et al. 1995. Proc Natl Acad Sci U S A. 92: 9097-101. PMID: 7568080
  7. 对多肽配体的转录反应:JAK-STAT 途径。  |  Schindler, C. and Darnell, JE. 1995. Annu Rev Biochem. 64: 621-51. PMID: 7574495
  8. 白细胞介素-3, 促红细胞生成素和催乳素可激活淋巴细胞中的 STAT5 类因子。  |  Pallard, C., et al. 1995. J Biol Chem. 270: 15942-5. PMID: 7608147
  9. Stat4,一种在早期骨髓分化过程中表达的新型γ干扰素激活位点结合蛋白。  |  Yamamoto, K., et al. 1994. Mol Cell Biol. 14: 4342-9. PMID: 8007943
  10. 白细胞介素-4 诱导的转录因子:IL-4 Stat.  |  Hou, J., et al. 1994. Science. 265: 1701-6. PMID: 8085155
  11. Stat3:一种 STAT 家族成员,在表皮生长因子和白细胞介素-6 的作用下通过酪氨酸磷酸化激活。  |  Zhong, Z., et al. 1994. Science. 264: 95-8. PMID: 8140422
  12. Jak-STAT 通路和转录激活对 IFNs 及其他细胞外信号蛋白的反应。  |  Darnell, JE., et al. 1994. Science. 264: 1415-21. PMID: 8197455

订购信息

产品名称产品编号规格价格数量收藏夹

Stat1 抗体 (C-136)

sc-464
200 µg/ml
RMB2377.00

Stat1 (C-136): m-IgGκ BP-HRP 套装

sc-520415
200 µg Ab, 40 µg BP
RMB2662.00

Stat1 抗体 (C-136) X

sc-464 X
200 µg/0.1 ml
RMB2377.00

Stat1 抗体 (C-136) AC

sc-464 AC
500 µg/ml, 25% agarose
RMB3129.00

Stat1 抗体 (C-136) HRP

sc-464 HRP
200 µg/ml
RMB2377.00

Stat1 抗体 (C-136) FITC

sc-464 FITC
200 µg/ml
RMB2482.00

Stat1 抗体 (C-136) PE

sc-464 PE
200 µg/ml
RMB2580.00

Stat1 抗体 (C-136) Alexa Fluor® 488

sc-464 AF488
200 µg/ml
RMB2685.00

Stat1 抗体 (C-136) Alexa Fluor® 546

sc-464 AF546
200 µg/ml
RMB2685.00

Stat1 抗体 (C-136) Alexa Fluor® 594

sc-464 AF594
200 µg/ml
RMB2685.00

Stat1 抗体 (C-136) Alexa Fluor® 647

sc-464 AF647
200 µg/ml
RMB2685.00

Stat1 抗体 (C-136) Alexa Fluor® 680

sc-464 AF680
200 µg/ml
RMB2685.00

Stat1 抗体 (C-136) Alexa Fluor® 790

sc-464 AF790
200 µg/ml
RMB2685.00

Hi there, I would like to ask if we can have a test sample of antibody for STAT1 and phosphorylated STAT1 from your company? Thanks a lot!

Asked by: Anonymous
Thank you for your question. Please contact our technical service team directly.
Answered by: Tech Support Europe
Date published: 2024-07-01

Does this antibody works on non-reducing conditions?

Asked by: Nmn002
Thank you for your question. We have not tested this antibody for non-reducing conditions, so I am unable to say if it will work or not. We recommend reducing/denaturing conditions for Westen blotting.
Answered by: Tech Service
Date published: 2019-10-23

Hello, could I have a sample of this antibody sc-464, to test for IF? the reviews are great and I would get it, but there is no review mentioning good IF, thanks! Charlotte 

Asked by: CharlotteO
Thank you very much for your inquiry. Please send us an email to [email protected] with the following information: - complete address for shipping - telephone, fax number and e-mail address - a contact person the products should be addressed to Remember once you try the sample, you can write a review in our web about it, indicating all the information you think might be useful for other researchers.
Answered by: Technical Support Europe
Date published: 2017-09-25

I want to perform some immunostaining in my mouse samples. How can I do that with a mouse monoclonal?

Asked by: Cweed
We recommend using a directly conjugated primary antibody. This product, sc-464, is available directly conjugated to AF488, AF647, and FITC.
Answered by: Technical Support 13
Date published: 2017-01-04
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Rated 5 out of 5 by from Very good antibodyVery good antibody for STAT1 detection at the expected molecular weight. I used melanoma cell lysate, 40 ug protein/lane, PVDF membrane, blocking in 5% milk in TBST, over night incubation in 5% milk at 1:500 concentration. Detection was performed with Super Signal West Fempto.
Date published: 2022-06-17
Rated 4 out of 5 by from Works !I bought this antibody for a coworker. Got nice results with the recommended dilution for western blot.
Date published: 2021-04-09
Rated 1 out of 5 by from Not suitable for WB on mouse cell linesI tested this antibody on different mouse cell line and no signal was detected.
Date published: 2019-11-26
Rated 5 out of 5 by from Works great!Works great! used at 1:200 and it gave nice strong bands for mouse and human tissue lysates.
Date published: 2018-03-22
Rated 5 out of 5 by from GreatThis antibody is great, and we will buy it in the future!
Date published: 2018-02-14
Rated 2 out of 5 by from It can not be detected in LPS plus IFN induced RAWIt can not be detected in LPS plus IFN induced RAW
Date published: 2018-02-05
Rated 5 out of 5 by from recommendedThe effect of Caki-1 whole cell lysate on mice was good, the dilution ratio of 1/1000 WB was very reliable and had a very low background.
Date published: 2017-10-31
Rated 3 out of 5 by from stat1抗体点评9月份申购了这批抗体,到货速度还是可以滴,效果算一般吧,有杂带,其他的几支抗体试用装还是有不错的,谢谢
Date published: 2017-10-23
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Stat1 抗体 (C-136) is rated 4.3 out of 5 by 36.
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