Date published: 2025-9-7

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P-Selectin/CD62P/SELP 抗体 (1 E 3): sc-19672

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说明书
  • P-Selectin/CD62P/SELP抗体(1 E 3)是小鼠单克隆IgG2a κ, 在13篇文献中引用,规格为200 µg/ml
  • 针对固定凝血酶活化的human血小板
  • P-Selectin/CD62P/SELP 抗体 (1 E 3) 推荐用于WB, IP, IF, IHC(P) 和 FCM,检测human 来源的 P-Selectin
  • 抗P-Selectin/CD62P/SELP抗体(1 E 3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取P-Selectin/CD62P/SELP (1 E 3): sc-19672免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG2a BP-HRP 是 P-Selectin/CD62P/SELP 抗体 (1 E 3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与P-Selectin/CD62P/SELP 抗体 (1 E 3) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

P-选择素抗体(1 E 3)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、石蜡包埋切片免疫组织化学(IHCP)和流式细胞术(FCM)检测人类来源的P-选择素。P-Selectin (1 E 3) 抗体有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。P-选择素(也称为GMP-140或CD62P)在炎症反应期间调节血小板和白细胞与内皮细胞粘附方面起着至关重要的作用,这对于将免疫细胞招募到损伤或感染部位至关重要。P-选择素主要位于活化的血小板和内皮细胞表面,在细胞粘附和迁移的初始阶段,P-选择素发挥着促进作用。P-选择素的正确功能对于维持血管完整性和调节免疫反应至关重要,因此P-选择素成为心血管疾病和炎症研究的重要目标。P-选择素与中性粒细胞和单核细胞上的特定碳水化合物相互作用,并且P-选择素的表达会因炎症刺激而上调,这凸显了P-选择素在各种疾病病理生理学中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

P-Selectin/CD62P/SELP 抗体 (1 E 3)

sc-19672
200 µg/ml
RMB2377.00

P-Selectin/CD62P/SELP (1 E 3): m-IgG Fc BP-HRP 套装

sc-525498
200 µg Ab; 10 µg BP
RMB2662.00

P-Selectin/CD62P/SELP (1 E 3): m-IgG2a BP-HRP 套装

sc-546291
200 µg Ab; 10 µg BP
RMB2662.00

P-Selectin/CD62P/SELP 抗体 (1 E 3) AC

sc-19672 AC
500 µg/ml, 25% agarose
RMB3129.00

P-Selectin/CD62P/SELP 抗体 (1 E 3) HRP

sc-19672 HRP
200 µg/ml
RMB2377.00

P-Selectin/CD62P/SELP 抗体 (1 E 3) FITC

sc-19672 FITC
200 µg/ml
RMB2482.00

P-Selectin/CD62P/SELP 抗体 (1 E 3) PE

sc-19672 PE
200 µg/ml
RMB2580.00

P-Selectin/CD62P/SELP 抗体 (1 E 3) Alexa Fluor® 488

sc-19672 AF488
200 µg/ml
RMB2685.00

P-Selectin/CD62P/SELP 抗体 (1 E 3) Alexa Fluor® 546

sc-19672 AF546
200 µg/ml
RMB2685.00

P-Selectin/CD62P/SELP 抗体 (1 E 3) Alexa Fluor® 594

sc-19672 AF594
200 µg/ml
RMB2685.00

P-Selectin/CD62P/SELP 抗体 (1 E 3) Alexa Fluor® 647

sc-19672 AF647
200 µg/ml
RMB2685.00

P-Selectin/CD62P/SELP 抗体 (1 E 3) Alexa Fluor® 680

sc-19672 AF680
200 µg/ml
RMB2685.00

P-Selectin/CD62P/SELP 抗体 (1 E 3) Alexa Fluor® 790

sc-19672 AF790
200 µg/ml
RMB2685.00

Regarding my question about another antibody, I'm not looking for a secondary antibody, but for another P-selectin antibody that could be used as the detector in ELISA that would bind at a spatially distinct epitope. Can you recommend one? Thanks

Asked by: tracey01
Thank you for your question. We do not characterize pairs of monoclonal antibodies for use in sandwich ELISA. Many of our antibodies are recommended as detection antibodies in direct or indirect ELISA, however we cannot recommend a matching capture antibody. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Technical Service
Date published: 2017-04-20

Do you have any data on performance in ELISA? If the antibody is known or predicted to work in ELISA, can you recommend a second antibody to use in the ELISA pair? Thank you

Asked by: tracey01
Thank you for your question. We have not yet tested this specific mouse monoclonal, P-Selectin Antibody (1 E 3): sc-19672, for ELISA so we do not have any specific recommendations we can offer at this time for this research application. However, the antibody has been tested in Western blot, immunohistochemistry and flow cytometry so we believe it should also work for ELISA. P-Selectin Antibody (1 E 3) is a mouse monoclonal IgG2a, so for a secondary antibody you would want to use an anti-mouse IgG secondary antibody conjugated to either alkaline phosphatase (AP) or horseradish peroxidase (HRP), depending on what detection method being used. We would recommend our mouse IgG binding protein conjugated to HRP, m-IgG kappa BP-HRP: sc-516102. A link to the product description is provided below for your reference. https://www.scbt.com/scbt/product/m-igg-kappa-bp-hrp
Answered by: Technical Support
Date published: 2017-04-18

Can P-Selectin (1 E 3): sc-19672 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, P-Selectin (1 E 3): sc-19672 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24

Dear Mr. or Ms. We would like to know if this CD62P antibody cross reacts with canine platelets.  I look forward to hearing from you.

Asked by: Peres Badial
Thank you for your question. We do not know whether or not this antibody will detect canine. P-Selectin Antibody (1 E 3) is a mouse monoclonal raised against fixed thrombin-activated human platelets. The exact epitope recognized by this antibody has not been determined so we are unable to try to predict cross-reactivity based on sequence analysis. This would have to be determined experimentally.
Answered by: Technical Support
Date published: 2017-02-08
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Rated 5 out of 5 by from Strong WB signal obtained in human plateletStrong WB signal obtained in human platelet extract. -SCBT QC
Date published: 2015-05-18
Rated 4 out of 5 by from Robust immunoperoxidase membrane stainingRobust immunoperoxidase membrane staining in formalin-fixed human platelets and white blood cells. -SCBT QC
Date published: 2015-03-23
Rated 4 out of 5 by from WB: Worked well in human SV40 cellsWB: Worked well in human SV40 cells. -SCBT Publication Review
Date published: 2015-01-26
Rated 5 out of 5 by from Published IF data with HUVECsPublished IF data with HUVECs -SCBT Publication Review
Date published: 2015-01-24
Rated 5 out of 5 by from Good quality antibody for human paraffin samplesGood quality antibody for human paraffin samples. Published in baboon artery (ductus arteriorus). -SCBT Publication Review
Date published: 2015-01-03
Rated 4 out of 5 by from Wonderful FCM data of human plateletsWonderful FCM data of human platelets. -SCBT QC
Date published: 2014-05-05
Rated 4 out of 5 by from Nice indirect FCM data of human plateletsNice indirect FCM data of human platelets stained with P-Selectin . -SCBT QC
Date published: 2014-05-05
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P-Selectin/CD62P/SELP抗体 (1 E 3) is rated 4.4 out of 5 by 7.
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