Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
P-Selectin/CD62P/SELP Antibody (CTB201) - Western Blotting - Image 5277
P-选择素抗体 (CTB201): sc-8419. 人血小板提取物中 P-选择素表达的 Western 印迹分析.
P-选择素抗体 (CTB201): sc-8419. 人血小板提取物中 P-选择素表达的 Western 印迹分析.

P-Selectin/CD62P/SELP 抗体 (CTB201): sc-8419

4.8(16)
寫評論提問

说明书
  • P-Selectin/CD62P/SELP 抗体 CTB201 是小鼠单克隆 IgG1 κ，P-Selectin/CD62P/SELP抗体, 在50篇文献中引用，规格为200 µg/ml
  • 免疫human物种的P-Selectin
  • P-Selectin/CD62P/SELP 抗体 (CTB201) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 P-Selectin
  • P-Selectin/CD62P/SELP 抗体 (CTB201) 可偶联 琼脂糖 (sc-8419 AC), 500 µg/ml, 25% agarose, 用于 IP; 还可偶联 藻红蛋白 (sc-8419 PE) 或者 FITC (sc-8419 FITC), 200 µg/ml, 用于 IF, IHC(P) 和 FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取P-Selectin/CD62P/SELP (CTB201): sc-8419的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 P-Selectin/CD62P/SELP 抗体 (CTB201) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与P-Selectin/CD62P/SELP 抗体 (CTB201) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Selectin抗体，包括E-Selectin, L-Selectin和P-Selectin

P-选择素抗体（CTB201）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）检测小鼠、大鼠和人类来源的P-选择素蛋白。P-选择素抗体（CTB201）有非结合型和多种结合型，包括琼脂糖、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物，可满足不同实验需求。P-选择素（也称为GMP-140或CD62P）在炎症反应中起着至关重要的作用，它通过介导白细胞与活化的内皮细胞和血小板的粘附，从而将免疫细胞招募到损伤或感染部位。P-选择素主要位于活化的血小板和内皮细胞表面，它促进白细胞的滚动和粘附，这是免疫反应的关键步骤。P-选择素正常运作对于维持血管完整性和确保有效的免疫监控至关重要，因此P-选择素成为炎症和心血管疾病研究的重要目标。抗P-选择素抗体（CTB201）是研究这些过程和理解各种病理潜在机制的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

P-Selectin/CD62P/SELP 抗体 (CTB201) 参考文献:

  1. P-选择素：心血管疾病、炎症和肿瘤转移的常见治疗靶点。  |  Ludwig, RJ., et al. 2007. Expert Opin Ther Targets. 11: 1103-17. PMID: 17665981
  2. 分子成像中靶向P-选择素粘附分子：P-选择素表达作为心血管疾病中炎症的重要成像生物标记物。  |  Perkins, LA., et al. 2019. J Nucl Med. 60: 1691-1697. PMID: 31601694
  3. 阻塞性睡眠呼吸暂停患者循环中的P-选择素糖蛋白配体1和P-选择素水平。  |  Horváth, P., et al. 2020. Lung. 198: 173-179. PMID: 31897593
  4. 以 P-选择素为靶标的 RAGE-shRNA 脂质体可缓解动脉粥样硬化相关炎症。  |  Mocanu, CA., et al. 2021. J Control Release. 338: 754-772. PMID: 34530051
  5. 选择素配体  |  Varki, A. 1994. Proc Natl Acad Sci U S A. 91: 7390-7. PMID: 7519775
  6. 由选择素-碳水化合物相互作用介导的白细胞迁移。  |  McEver, RP., et al. 1995. J Biol Chem. 270: 11025-8. PMID: 7538108
  7. L-选择素的细胞质结构域通过α-肌动蛋白与细胞骨架蛋白相互作用：受体在微绒毛中的定位不需要与α-肌动蛋白相互作用。  |  Pavalko, FM., et al. 1995. J Cell Biol. 129: 1155-64. PMID: 7538138
  8. 选择素：血管粘附分子。  |  Tedder, TF., et al. 1995. FASEB J. 9: 866-73. PMID: 7542213
  9. 选择素与碳水化合物的相互作用以及炎症反应的启动。  |  Lasky, LA. 1995. Annu Rev Biochem. 64: 113-39. PMID: 7574477
  10. 同时缺乏 P-和 E-选择素的小鼠易受感染，造血功能发生改变。  |  Frenette, PS., et al. 1996. Cell. 84: 563-74. PMID: 8598043
  11. 血小板介导的淋巴细胞向高内皮静脉输送。  |  Diacovo, TG., et al. 1996. Science. 273: 252-5. PMID: 8662511
  12. 两种选择蛋白汇聚在硫酸盐上。白细胞粘附。  |  Rosen, SD. and Bertozzi, CR. 1996. Curr Biol. 6: 261-4. PMID: 8805242

订购信息

产品名称产品编号规格价格数量收藏夹

P-Selectin/CD62P/SELP 抗体 (CTB201)

sc-8419
200 µg/ml
RMB2377.00

P-Selectin/CD62P/SELP (CTB201): m-IgG Fc BP-HRP 套装

sc-526522
200 µg Ab; 10 µg BP
RMB2662.00

P-Selectin/CD62P/SELP (CTB201): m-IgG1 BP-HRP 套装

sc-531895
200 µg Ab; 20 µg BP
RMB2662.00

P-Selectin/CD62P/SELP 抗体 (CTB201) AC

sc-8419 AC
500 µg/ml, 25% agarose
RMB3129.00

P-Selectin/CD62P/SELP 抗体 (CTB201) FITC

sc-8419 FITC
200 µg/ml
RMB2482.00

P-Selectin/CD62P/SELP 抗体 (CTB201) PE

sc-8419 PE
200 µg/ml
RMB2580.00

P-Selectin/CD62P/SELP 抗体 (CTB201) Alexa Fluor® 488

sc-8419 AF488
200 µg/ml
RMB2685.00

P-Selectin/CD62P/SELP 抗体 (CTB201) Alexa Fluor® 546

sc-8419 AF546
200 µg/ml
RMB2685.00

P-Selectin/CD62P/SELP 抗体 (CTB201) Alexa Fluor® 594

sc-8419 AF594
200 µg/ml
RMB2685.00

P-Selectin/CD62P/SELP 抗体 (CTB201) Alexa Fluor® 647

sc-8419 AF647
200 µg/ml
RMB2685.00

P-Selectin/CD62P/SELP 抗体 (CTB201) Alexa Fluor® 680

sc-8419 AF680
200 µg/ml
RMB2685.00

P-Selectin/CD62P/SELP 抗体 (CTB201) Alexa Fluor® 790

sc-8419 AF790
200 µg/ml
RMB2685.00

Can this antibody work on western blots of mouse kidney, lung or liver tissue? Anti-P-Selectin/CD62P/SELP Antibody (CTB201): sc-8419

Asked by: ssun
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-01-20

Can you maybe provide any information regarding the isoelectric point of this antibody or guide me in the right direction, please?

Asked by: Riaan
Thank you for your question. We do not characterize the isoelectric point for any of our antibodies. The isoelectric point (pI) of IgG is found in the range of 6.4 to 9.0 [1]. References: [1] Li G, Stewart R, Conlan B, Gilbert A, Roerth P, Nair H. Purification of human immunoglobulin G: A new approach to plasma fractionation. Vox Sanguinis (2002) 83, 332-338.
Answered by: Technical Support
Date published: 2019-07-12

For the dye conjugated antibodies (eg. P-selectin-FITC), will further conjugation still be available on that antibody? ie. does the antibody still have primary NH2 groups for other conjugation reactions?

Asked by: mich77
Thank you for your question. Most reactive/readily available primary amines have most likely have been conjugated. We can not say definitely, but there may be some available. However, we would not recommend attempting additional conjugation of the antibody since the antibody conjugates are provided with a stabilizing protein (gelatin) which would interfere with the conjugation.
Answered by: Technical Support
Date published: 2019-06-16

I purchased 4 antibodies against human PECAM, VE-Cadherin, Ang-2 and P-selectin for IF of endothelial cells, all first three antibodies worked well, but the P-selectin ( sc-8419) did not worked. I used same secondary antibodies for all of them.

Asked by: HamidehYaD
Thank you for your question. We are sorry to hear that you are not getting the expected result with one of our antibodies. Please contact our Technical Service by phone, email or by Live Chat. They will help you figure out how to resolve the issue and provide an appropriate replacement free of charge if the original antibody does not work.
Answered by: SCBT Heidelberg Support
Date published: 2018-12-30

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with P-Selectin Antibody (CTB201): sc-8419?

Asked by: Trav11
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-17

Does P-Selectin Antibody (CTB201): sc-8419 react with canine samples?

Asked by: Trav11
Thank you for your question. For additional species reactivity not listed on our website, please contact our Technical Service department by calling 800-457-3801 option 2, emailing [email protected], or by using the Live Chat function on our website and we will be more than happy to run sequence analysis for your species of interest.
Answered by: Technical Support 5
Date published: 2017-01-16
  • y_2025, m_9, d_7, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_6
  • loc_zh_CN, sid_8419, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 109ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from Tested on HFFF by FACSThis antibody was tested on HFFF cells by FACS and did not give a signal, even at dilution 1:25
Date published: 2018-11-19
Rated 5 out of 5 by from ExcellentAntibodies are used in human platelet extract, which are better than what I ordered from other suppliers and strong signals are diluted at high concentrations
Date published: 2017-10-31
Rated 5 out of 5 by from clear bandObtained a single clear band in Western Blot with 293T cells.
Date published: 2017-02-25
Rated 5 out of 5 by from Superb dataWorks well for FCM, IF and western blot applications
Date published: 2017-01-24
Rated 5 out of 5 by from Great monoclonal choice for P-SelectinStrong and specific, it's a great choice for IHC(P), FCM, IF or WB applications.
Date published: 2017-01-17
Rated 5 out of 5 by from Works very goodexcellent antibody, works by western blot at 1:1000 for both mouse and human lysates
Date published: 2016-12-17
Rated 5 out of 5 by from very goodWe often use P-Selectin antibody,used for Western blot detection of platelet extraction,always use domestic brands antibody, are colleagues recommend Santa monoclonal antibodies,Effect is very good.
Date published: 2016-12-17
Rated 5 out of 5 by from Use this routinely in WB and works wellUse this routinely in WB and works well.
Date published: 2015-07-10
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_16
  • loc_zh_CN, sid_8419, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 19ms
  • REVIEWS, PRODUCT
P-Selectin/CD62P/SELP 抗体 (CTB201) is rated 4.8 out of 5 by 16.
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_16
  • loc_zh_CN, sid_8419, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 107ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。