Date published: 2025-9-8

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p-Rsk 抗体 (C-5): sc-377526

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说明书
  • p-Rsk抗体(C-5)是小鼠单克隆IgG2a κ, 在5篇文献中引用,规格为200 µg/ml
  • 特异性表位,对应于含有磷酸化丝氨酸380的短氨基酸序列,来源于Rsk -1的human
  • 抗-p-Rsk 抗体 (C-5) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Ser 380 phosphorylated Rsk
  • 抗p-Rsk抗体(C-5)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p-Rsk (C-5): sc-377526免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRP是p-Rsk Antibody (C-5) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与p-Rsk Antibody (C-5) 打包提供(请参阅下面的订购信息)。

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関連項目

p-Rsk抗体(C-5)是一种IgG2a κ小鼠单克隆p-Rsk抗体,可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的Ser 380磷酸化Rsk。p-Rsk抗体(C-5)既有非偶联的抗p-Rsk抗体形式,也有多种偶联形式的抗p-Rsk抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。核糖体S6激酶(Rsks)家族,包括Rsk-1(或MAPKAP激酶-1)、Rsk-2和Rsk-3,是细胞内丝氨酸/苏氨酸激酶,是响应多种配体激活的受体酪氨酸激酶的重要信号中间体。Rsk家族成员的一个独特特征是每个成员都拥有两个非完全相同的激酶催化结构域。另一个Rsk蛋白,Rsk-4,与人类Rsk家族中先前分离的三个成员具有高度同源性。Rsk-4在脑和肾中的表达最为丰富,在正常神经元发育中发挥作用。核糖体S6激酶家族包括p70 S6激酶和p70 S6激酶β,它们被认为具有相似的调节功能。MSK1(也称为RLPK)是一种新型的与Rsk相关的蛋白质,与p90 Rsk家族成员一样,含有两个非完全相同的激酶催化结构域。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-Rsk 抗体 (C-5) 参考文献:

  1. 人类 RSK4(一种新的 90 kDa 核糖体 S6 激酶)的功能特性分析表明,RSK4 在大多数细胞类型中具有组成性激活作用。  |  Dümmler, BA., et al. 2005. J Biol Chem. 280: 13304-14. PMID: 15632195
  2. 克隆大鼠肝脏中的丝裂原活化 S6 激酶,发现它是第二信使亚家族的一种酶。  |  Kozma, SC., et al. 1990. Proc Natl Acad Sci U S A. 87: 7365-9. PMID: 1699226
  3. 卡波西肉瘤相关疱疹病毒 ORF45 持续激活核糖体 S6 激酶(RSK)和 ERK 的机制:多蛋白复合物保留了活性磷酸化 ERK 和 RSK,并保护它们免于去磷酸化。  |  Kuang, E., et al. 2009. J Biol Chem. 284: 13958-13968. PMID: 19304659
  4. Rsk 介导的 Apaf-1 磷酸化和 14-3-3ɛ 结合可抑制细胞色素 c 诱导的细胞凋亡。  |  Kim, J., et al. 2012. EMBO J. 31: 1279-92. PMID: 22246185
  5. 主要胰岛素/介素激活的 70-kDa S6 蛋白激酶的分子结构。  |  Banerjee, P., et al. 1990. Proc Natl Acad Sci U S A. 87: 8550-4. PMID: 2236064
  6. 鉴定鸡胚成纤维细胞中的有丝分裂原反应核糖体蛋白 S6 激酶 pp90rsk,它是爪蟾 S6 激酶 II 的同源物。  |  Sweet, LJ., et al. 1990. Mol Cell Biol. 10: 2413-7. PMID: 2325657
  7. 鸡和小鼠 rsk 的序列和表达:爪蟾核糖体 S6 激酶的同源物。  |  Alcorta, DA., et al. 1989. Mol Cell Biol. 9: 3850-9. PMID: 2779569
  8. CdGAP/ARHGAP31 受 RSK 磷酸化和与 14-3-3β 适应蛋白结合的调控。  |  Ben Djoudi Ouadda, A., et al. 2018. Oncotarget. 9: 11646-11664. PMID: 29545927
  9. mTORC2 参与血清或营养饥饿对 RSK 磷酸化的诱导  |  Chou, PC., et al. 2020. Cells. 9: PMID: 32605013
  10. RSK3 编码一种具有独特 N 端序列的新型 pp90rsk 异构体:生长因子刺激激酶功能和核转位。  |  Zhao, Y., et al. 1995. Mol Cell Biol. 15: 4353-63. PMID: 7623830
  11. p90rsk 激酶结构域的不同功能作用。  |  Bjørbaek, C., et al. 1995. J Biol Chem. 270: 18848-52. PMID: 7642538
  12. 人类 rsk 同工酶:组织特异性表达的克隆和表征。  |  Moller, DE., et al. 1994. Am J Physiol. 266: C351-9. PMID: 8141249

订购信息

产品名称产品编号规格价格数量收藏夹

p-Rsk 抗体 (C-5)

sc-377526
200 µg/ml
RMB2377.00

p-Rsk (C-5): m-IgG Fc BP-HRP 套装

sc-526108
200 µg Ab; 10 µg BP
RMB2662.00

p-Rsk (C-5): m-IgG2a BP-HRP 套装

sc-546505
200 µg Ab; 10 µg BP
RMB2662.00

p-Rsk 抗体 (C-5) AC

sc-377526 AC
500 µg/ml, 25% agarose
RMB3129.00

p-Rsk 抗体 (C-5) HRP

sc-377526 HRP
200 µg/ml
RMB2377.00

p-Rsk 抗体 (C-5) FITC

sc-377526 FITC
200 µg/ml
RMB2482.00

p-Rsk 抗体 (C-5) PE

sc-377526 PE
200 µg/ml
RMB2580.00

p-Rsk 抗体 (C-5) Alexa Fluor® 488

sc-377526 AF488
200 µg/ml
RMB2685.00

p-Rsk 抗体 (C-5) Alexa Fluor® 546

sc-377526 AF546
200 µg/ml
RMB2685.00

p-Rsk 抗体 (C-5) Alexa Fluor® 594

sc-377526 AF594
200 µg/ml
RMB2685.00

p-Rsk 抗体 (C-5) Alexa Fluor® 647

sc-377526 AF647
200 µg/ml
RMB2685.00

p-Rsk 抗体 (C-5) Alexa Fluor® 680

sc-377526 AF680
200 µg/ml
RMB2685.00

p-Rsk 抗体 (C-5) Alexa Fluor® 790

sc-377526 AF790
200 µg/ml
RMB2685.00

p-Rsk (C-5) 中和勝肽

sc-377526 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-377526 P appropriate for?

Asked by: Professor Griffin
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Muy buen resultado para WBMuy buen resultado para WB. La banda es específica en extractos celulares totales de células AGS.
Date published: 2018-01-11
Rated 4 out of 5 by from Works well for WBworks well for western - samples were human cells in culture
Date published: 2017-11-21
Rated 4 out of 5 by from Good antibody for Western blottingThis monoclonal antibody was independently tested by Kinexus Bioinformatics Corporation and was determined to be suitable for Western blotting. It provided medium detection of the Rsk target in immunoblots of lysates from HeLa cells that were exposed to anisomycin for 2 hours, but no other cross-reactivities were observed. There was no detection of the Rsk target in overnight serum-starved human A431 cells treated for 5 minutes with epidermal growth factor on Western blots, but again this antibody was very clean and no significant cross-reactivities on these blots with any off-target proteins.
Date published: 2016-12-08
Rated 5 out of 5 by from Nice Western blot data of Rsk phosphorylationNice Western blot data of Rsk phosphorylation in untreated, PMA treated and PMA and lambda protein phosphatase treated HeLa whole cell lysates. -SCBT QC
Date published: 2013-11-03
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p-Rsk 抗体 (C-5) is rated 4.5 out of 5 by 4.
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