Date published: 2025-9-7
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p-mTOR Antibody (59.Ser 2448) - Western Blotting - Image 374900
p-mTOR 抗体 (59. Ser 2448): sc-293133. HeLa (A), CCRF-CEM (B) 和 TK-1 (C) 全细胞裂解液中 mTOR 磷酸化的 Western 印迹分析.
p-mTOR 抗体 (59. Ser 2448): sc-293133. HeLa (A), CCRF-CEM (B) 和 TK-1 (C) 全细胞裂解液中 mTOR 磷酸化的 Western 印迹分析.

p-mTOR 抗体 (59.Ser 2448): sc-293133

4.5(49)
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说明书
  • p-mTOR抗体(59.Ser 2448)是小鼠单克隆IgG1 κ, 在141篇文献中引用，规格为200 µg/ml
  • 针对含磷酸化Ser 2448的短氨基酸序列的抗体，该序列来自mTOR的human起源
  • 推荐用于 mouse, rat 和 human 来源的Ser 2448 phosphorylated mTOR WB, IP, IF, IHC(P) 和 ELISA检测
  • 还可偶联琼脂糖 用于IP; 偶联HRP 用于WB, IHC(P) 和ELISA
  • 关于如何获取p-mTOR (59.Ser 2448): sc-293133的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 p-mTOR 抗体 (59.Ser 2448) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与p-mTOR 抗体 (59.Ser 2448) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

p-mTOR抗体（59.Ser 2448）是一种IgG1 κ小鼠单克隆p-mTOR抗体，可以通过蛋白质印迹法（WB）、免疫沉淀法（IP）、免疫荧光法（IF）、免疫组织化学法（IHC-P）和酶联免疫吸附测定法（ELISA）检测小鼠、大鼠和人类来源的Ser 2448磷酸化的mTOR。p-mTOR抗体（59.Ser 2448）既有偶联形式，也有非偶联形式的抗p-mTOR抗体。PIK相关激酶包括Atm、DNA-PKCS和mTOR。Atm基因在常染色体隐性遗传疾病共济失调毛细血管扩张症（AT）中发生突变，该疾病以小脑退化和眼结膜血管扩张为特征。AT细胞对电离辐射超敏感，介导DNA合成的抑制能力受损，并且显示p53诱导的延迟。DNA-PK是一种异三聚体DNA结合酶，由一个大亚基DNA-PKCS和两个较小亚基（统称为Ku）组成。DNA-PK的丧失会导致DSB修复和V(D)J重组的缺陷。mTOR，也称为FRAP，可以在丝氨酸上自磷酸化并结合雷帕霉素/FKBP。mTOR也是S6激酶的上游调节因子，并已涉及p27和p21表达的调节。在翻译抑制条件下，mTOR会在Ser2481处自磷酸化。而mTOR在Ser2448处的磷酸化则是由p70S6激酶介导的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-mTOR 抗体 (59.Ser 2448) 参考文献:

  1. 心脏起搏患者的数据显示记录。  |  Levander-Lindgren, M. 1976. Acta Med Scand Suppl. 596: 77-9. PMID: 1070231
  2. 哺乳动物雷帕霉素靶标（mTOR）在 Ser-2448 处的磷酸化是由 p70S6 激酶介导的。  |  Chiang, GG. and Abraham, RT. 2005. J Biol Chem. 280: 25485-90. PMID: 15899889
  3. 磷酸化mTOR及其调节蛋白的表达与牙釉质瘤的生物学行为有关。  |  Li, N., et al. 2012. Int J Clin Exp Pathol. 5: 660-7. PMID: 22977662
  4. mTOR和p-mTOR在胃肠道间质瘤中的作用。  |  Li, JC., et al. 2013. Asian Pac J Cancer Prev. 14: 5925-8. PMID: 24289602
  5. 磷酸化的mTOR表达及其在小细胞肺癌中的临床意义。  |  Lee, JH., et al. 2015. Int J Clin Exp Pathol. 8: 2987-93. PMID: 26045808
  6. 什么情况下脂质激酶不是脂质激酶？当它是一种蛋白激酶时。  |  Hunter, T. 1995. Cell. 83: 1-4. PMID: 7553860
  7. PIK 相关激酶：DNA 修复, 重组和细胞周期检查点。  |  Keith, CT. and Schreiber, SL. 1995. Science. 270: 50-1. PMID: 7569949
  8. DNA 依赖性蛋白激酶催化亚基：磷脂酰肌醇 3-激酶和共济失调毛细血管扩张症基因产物的亲缘关系。  |  Hartley, KO., et al. 1995. Cell. 82: 849-56. PMID: 7671312
  9. AT基因的发现引发了生物医学研究的繁荣。  |  Nowak, R. 1995. Science. 268: 1700-1. PMID: 7792589
  10. 单个共济失调毛细血管扩张症基因的产物与 PI-3 激酶相似。  |  Savitsky, K., et al. 1995. Science. 268: 1749-53. PMID: 7792600

订购信息

产品名称产品编号规格价格数量收藏夹

p-mTOR 抗体 (59.Ser 2448)

sc-293133
200 µg/ml
RMB2377.00

p-mTOR (59.Ser 2448): m-IgG Fc BP-HRP 套装

sc-527321
200 µg Ab; 10 µg BP
RMB2662.00

p-mTOR (59.Ser 2448): m-IgG1 BP-HRP 套装

sc-532694
200 µg Ab; 20 µg BP
RMB2662.00

p-mTOR 抗体 (59.Ser 2448) AC

sc-293133 AC
500 µg/ml, 25% agarose
RMB3129.00

p-mTOR 抗体 (59.Ser 2448) HRP

sc-293133 HRP
200 µg/ml
RMB2377.00

This antibody is to be used for immunohistochemistry in rat liver tissue, is the recommended dilution concentration 1:50 or 1:500?

Asked by: Tung
Thank you for your question. The recommended dilution range is between 1:50 and 1:500. Optimum conditions for your particular experimental setup will have to be determined by titration.
Answered by: Tech Support Europe
Date published: 2022-09-05

Is this antibody suitable for IHC staining (whole mount) and what dilution do you recommend? Thank you!

Asked by: Pedro1816
Thank you for your question. Anti-p-mTOR Antibody (59.Ser 2448): sc-293133 has only been tested for IHC stainings with tissue sections from mouse, rat, or human. There is no internal data for whole mount stainings that would support such an experiment.
Answered by: Tech Support Europe
Date published: 2022-02-11

Me gustaría recibir una muestra gratuita del anticuerpo p-mTOR (59.Ser 2448)

Asked by: Mónica33
Gracias por su pregunta. Por favor contacte Servicio Tecnico para obtener mas informacion en como recibir una muestra de 10 μg de p-mTOR anticuerpo. Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-06

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: jerojero
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-13

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with p-mTOR (59.Ser 2448): sc-293133?

Asked by: jerojero
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 13
Date published: 2017-01-13
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Rated 4 out of 5 by from Good for WBWork fine in western blot analysis in A498 ccRCC cells
Date published: 2024-11-26
Rated 5 out of 5 by from 该抗体可以检测绵羊肝脏和十二指肠蛋白表达p-mTOR 抗体 (59.Ser 2448)可以用于检测绵羊肝脏和十二指肠p-mTOR蛋白表达，采用WB。
Date published: 2024-10-26
Rated 4 out of 5 by from Good for western blotWe used it to detect phospho mTOR in several human cancer cell lines by western blot, and worked well at 1:500 dilution
Date published: 2023-07-17
Rated 4 out of 5 by from Good wester blot signal!Good western blot signal on mouse tissue samples at 1:1000 dilution
Date published: 2022-03-24
Rated 5 out of 5 by from ottimo funzionamentoMolto soddisfatta per l'acquisto dell'anticorpo sc-293133
Date published: 2021-10-04
Rated 5 out of 5 by from 功能不错产品发给客户，到目前没有不良反馈；订货时，申请了三个试用装，同样也没有收到不佳的反馈。有小样试用装给客户很大的吸引力
Date published: 2021-08-07
Rated 5 out of 5 by from Good for western blotGood western blot signal on mouse tissue samples at 1:1000 dilution
Date published: 2020-10-06
Rated 5 out of 5 by from Good antibody for western blottingp‐mTOR‐S2448 (sc‐293133) is high quantly and low background for western blotting
Date published: 2020-05-25
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p-mTOR 抗体 (59.Ser 2448) is rated 4.5 out of 5 by 49.
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