Date published: 2025-9-7

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p-mTOR 抗体 (296.Ser 2481): sc-293132

3.5(6)
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说明书
  • p-mTOR抗体(296.Ser 2481)是小鼠单克隆IgG2b κ, 在37篇文献中引用,规格为200 µg/ml
  • 针对含磷酸化Ser 2481的短氨基酸序列的抗体,该序列来自mTOR的human起源
  • 推荐用于 mouse, rat 和 human 来源的Ser 2481 phosphorylated mTOR WB, IP, IF, IHC(P) 和 ELISA检测
  • 还可偶联琼脂糖 用于IP; 偶联HRP 用于WB, IHC(P) 和ELISA
  • 关于如何获取p-mTOR (296.Ser 2481): sc-293132免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG2b BP-HRP 是 p-mTOR 抗体 (296.Ser 2481) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与p-mTOR 抗体 (296.Ser 2481) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

p-mTOR抗体(296.Ser 2481)是一种IgG2b κ小鼠单克隆p-mTOR抗体,可以通过蛋白质印迹法(WB)、免疫沉淀法(IP)、免疫荧光法(IF)、免疫组织化学法(IHC-P)和酶联免疫吸附测定法(ELISA)检测小鼠、大鼠和人类来源的Ser 2481磷酸化的mTOR。p-mTOR抗体(296.Ser 2481)既有偶联形式,也有非偶联形式的抗p-mTOR抗体。PIK相关激酶包括Atm、DNA-PKCS和mTOR。Atm基因在常染色体隐性遗传疾病共济失调毛细血管扩张症(AT)中会发生突变,该疾病以小脑退化和眼结膜血管扩张为特征。AT细胞对电离辐射超敏感,在介导DNA合成的抑制方面存在缺陷,并且显示p53诱导的延迟。DNA-PK是一种异三聚体DNA结合酶,由一个大亚基DNA-PKCS和两个较小亚基(统称为Ku)组成。DNA-PK的丧失会导致DSB修复和V(D)J重组的缺陷。mTOR,也称为FRAP,可以在丝氨酸上自磷酸化并结合雷帕霉素/FKBP。mTOR也是S6激酶的上游调节因子,并已涉及p27和p21表达的调节。在翻译抑制条件下,mTOR会在Ser2481处自磷酸化。而mTOR在Ser2448处的磷酸化则是由p70S6激酶介导的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

p-mTOR 抗体 (296.Ser 2481)

sc-293132
200 µg/ml
RMB2377.00

p-mTOR (296.Ser 2481): m-IgG Fc BP-HRP 套装

sc-525848
200 µg Ab; 10 µg BP
RMB2662.00

p-mTOR (296.Ser 2481): m-IgG2b BP-HRP 套装

sc-549635
200 µg Ab; 10 µg BP
RMB2662.00

p-mTOR 抗体 (296.Ser 2481) AC

sc-293132 AC
500 µg/ml, 25% agarose
RMB3129.00

p-mTOR 抗体 (296.Ser 2481) HRP

sc-293132 HRP
200 µg/ml
RMB2377.00

Is there data showing this antibody is specific to phosphorylated mTOR?

Asked by: AbPolly
Thank you for your question. We have Western Blot data showing sensitivity to Calyculin A treatment and lambda phosphatase treatment which shows this antibody is specific to phosphorylated mTOR.
Answered by: Technical Support 5
Date published: 2017-01-25
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Rated 1 out of 5 by from Doesn't workI repeated WB for several times. The Ab doen't work.
Date published: 2018-02-21
Rated 4 out of 5 by from 很好出条带条带很好出,稀释比例1:200,但是至少有三条的杂带,不像说明书上那么单一干净,不知道是什么原因。。
Date published: 2018-01-26
Rated 1 out of 5 by from Did not work with Licor systemI tried performing WB in HEK293T cells in 1:500 dilution and used anti- M IgG (H&L)-680 (1:10,000). However I did not observe any band. A different secondary antibody may be needed.
Date published: 2017-11-13
Rated 5 out of 5 by from Very satisfied1:600 dilution was very strong and specific in the tissues of WB rats, and it worked well in WB experiment.
Date published: 2017-10-26
Rated 5 out of 5 by from Great for IHCPp-mTOR Antibody (296.Ser 2481) worked great in our IHC (P) experiment. Very nice staining observed
Date published: 2017-01-13
Rated 5 out of 5 by from Worked well with single band for WB in untreatedWorked well with single band for WB in untreated (A,D), Calyculin A treated (B,E) and Calyculin A and lambda protein phosphatase treated (C,F) Jurkat whole cell lysates. -SCBT QC
Date published: 2013-06-20
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p-mTOR 抗体 (296.Ser 2481) is rated 3.5 out of 5 by 6.
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