Date published: 2025-9-7

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p-Met 抗体 (F-5): sc-377548

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说明书
  • p-Met抗体(F-5)是小鼠单克隆IgG3 κ, 在7篇文献中引用,规格为200 µg/ml
  • 针对human起源的含酪氨酸1365的磷酸化甲硫氨酸的短氨基酸序列
  • 推荐用于 mouse, rat, human 和 来源的Tyr 1365 phosphorylated Met WB, IP, IF, IHC(P) 和 ELISA检测; 也和以下物种反应,包括: 和 equine, canine, bovine, porcine and avian
  • 关于如何获取p-Met (F-5): sc-377548免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成p-Met 抗体 (F-5)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

p-Met抗体(F-5)是一种IgG3 κ小鼠单克隆p-Met抗体(也称为p-Met抗体或c-Met抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的Tyr 1365磷酸化Met。p-Met抗体(F-5)有非偶联的抗p-Met抗体形式可供选择。c-Met癌基因最初是通过在NIH/3T3细胞中转染分析从化学致癌物处理的人成骨肉瘤细胞系中分离出来的。Met原癌基因产物被鉴定为一种跨膜受体样蛋白,具有酪氨酸激酶活性,在许多组织中表达。高比例的自发性 NIH/3T3 转化体过表达 c-Met,通过转染分析,c-Met 原癌基因已被证明具有转化活性。在某些人胃癌细胞系中也观察到明显正常的 Met 蛋白的酪氨酸磷酸化。c-Met基因产物已被确定为肝细胞生长因子的细胞表面受体,肝细胞生长因子是一种纤溶酶原样蛋白,被认为是肝脏再生的体液介质。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-Met 抗体 (F-5) 参考文献:

  1. 确定肝细胞生长因子受体为 c-met 原癌基因产物。  |  Bottaro, DP., et al. 1991. Science. 251: 802-4. PMID: 1846706
  2. MET和磷酸化MET作为肺癌的潜在生物标志物。  |  Tretiakova, M., et al. 2011. J Environ Pathol Toxicol Oncol. 30: 341-54. PMID: 22181983
  3. met 致癌基因的激活机制。  |  Park, M., et al. 1986. Cell. 45: 895-904. PMID: 2423252
  4. c-MET、p-MET和E2f-1在人类胃癌中的表达和临床意义。  |  Wu, JG., et al. 2014. BMC Res Notes. 7: 6. PMID: 24393368
  5. 小鼠 met 原癌基因的特征。  |  Chan, AM., et al. 1988. Oncogene. 2: 593-9. PMID: 2838789
  6. 自发转化的 NIH3T3 小鼠成纤维细胞中 met 基因的扩增和过表达。  |  Cooper, CS., et al. 1986. EMBO J. 5: 2623-8. PMID: 3023053
  7. p145,一种在人类胃癌细胞系中具有相关酪氨酸激酶活性的蛋白质。  |  Giordano, S., et al. 1988. Mol Cell Biol. 8: 3510-7. PMID: 3211149
  8. 福瑞替尼通过下调 p-MET/HGF 信号在体内外对三阴性乳腺癌细胞 MDA-MB-231 均有效  |  Ji, X., et al. 2023. Int J Mol Sci. 24: PMID: 36614199
  9. 从化学转化的人类细胞系中分子克隆出一种新的转化基因。  |  Cooper, CS., et al. Nature. 311: 29-33. PMID: 6590967
  10. 丝氨酸 985 的磷酸化对肝细胞生长因子受体激酶有负向调节作用。  |  Gandino, L., et al. 1994. J Biol Chem. 269: 1815-20. PMID: 8294430

订购信息

产品名称产品编号规格价格数量收藏夹

p-Met 抗体 (F-5)

sc-377548
200 µg/ml
RMB2377.00

p-Met (F-5) 中和勝肽

sc-377548 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-377548 P appropriate for?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Work for IF on cryosectionsGot a super clean stain for IF on cryosections of mouse kidney. Haven't tested yet in WB, but so far really happy with it.
Date published: 2019-04-19
Rated 2 out of 5 by from BadDid not work in my Hela cells, I tried it with 1:500 dilutions, and positive control did not work
Date published: 2018-04-12
Rated 1 out of 5 by from No signalThis antibody (sc-377548) did not give a signal on a western blot when another antibody that we used before (sc-101736) gave good signal but it has been terminated.
Date published: 2017-11-10
Rated 4 out of 5 by from 1:500 dilutionI obtained a good and specific signal in WB using this dilution
Date published: 2017-10-24
Rated 2 out of 5 by from Doesn't work in canine samplesDoesn't work in canine samples of osteosarcoma canine cell lines.
Date published: 2017-09-18
Rated 5 out of 5 by from Produced positive Western blot data of MetProduced positive Western blot data of Met phosphorylation in untreated, pervanadate treated and pervanadate and lambda protein phosphatase treated A-431 whole cell lysates. -SCBT QC
Date published: 2014-08-06
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p-Met 抗体 (F-5) is rated 3.2 out of 5 by 6.
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