Date published: 2025-9-7

021-6093-6350

SCBT Portrait Logo
Seach Input

Met 抗体 (B-2): sc-8057

4.1(15)
寫評論提問

说明书
  • Met 抗体 B-2 是小鼠单克隆 IgG1 κ,Met抗体, 在125篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于mouse物种的c-Met p140的C-terminus的氨基酸1359-1379之间
  • Met 抗体 (B-2) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse 和rat 来源的 Met
  • 抗Met抗体(B-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Met (B-2): sc-8057免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 Met 抗体 (B-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Met 抗体 (B-2) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

関連項目

Met抗体(B-2)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA)检测小鼠和大鼠来源的Met。Met单克隆抗体(B-2)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物,可满足不同实验需求。Met,又称c-Met,是一种具有天然酪氨酸激酶活性的跨膜受体,在细胞增殖、运动和形态发生等过程中发挥着关键作用。在组织再生和修复方面,Met的功能尤为重要,因为Met是肝细胞生长因子的受体,而肝细胞生长因子是肝脏再生过程中的关键因子。c-Met信号传导失调与多种癌症有关,因此Met成为治疗干预的重要靶点。c-Met 癌基因最初是通过转染分析从化学诱导的人类骨肉瘤衍生出的 NIH/3T3 细胞中鉴定的,这凸显了 Met 在肿瘤发生中的重要性。此外,在某些人类胃癌细胞系中,Met 存在酪氨酸磷酸化,这强调了 Met 在肿瘤进展和转移中的潜在作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Met 抗体 (B-2) 参考文献:

  1. c-Met 和肝细胞生长因子:作为癌症治疗新靶点的潜力。  |  Sattler, M. and Salgia, R. 2007. Curr Oncol Rep. 9: 102-8. PMID: 17288874
  2. 利用分子靶向磁共振成像技术在大鼠肝癌模型中检测 c-MET 的体内表达。  |  Towner, RA., et al. 2007. Mol Imaging. 6: 18-29. PMID: 17311762
  3. 确定肝细胞生长因子受体为 c-met 原癌基因产物。  |  Bottaro, DP., et al. 1991. Science. 251: 802-4. PMID: 1846706
  4. met 致癌基因的激活机制。  |  Park, M., et al. 1986. Cell. 45: 895-904. PMID: 2423252
  5. 小鼠 met 原癌基因的特征。  |  Chan, AM., et al. 1988. Oncogene. 2: 593-9. PMID: 2838789
  6. 自发转化的 NIH3T3 小鼠成纤维细胞中 met 基因的扩增和过表达。  |  Cooper, CS., et al. 1986. EMBO J. 5: 2623-8. PMID: 3023053
  7. p145,一种在人类胃癌细胞系中具有相关酪氨酸激酶活性的蛋白质。  |  Giordano, S., et al. 1988. Mol Cell Biol. 8: 3510-7. PMID: 3211149
  8. 靶向癌症中的MET失调。  |  Recondo, G., et al. 2020. Cancer Discov. 10: 922-934. PMID: 32532746
  9. MET在化疗耐药性中的作用。  |  Wood, GE., et al. 2021. Oncogene. 40: 1927-1941. PMID: 33526881
  10. MET激活泛素多聚体。  |  Kawakami, N., et al. 2023. Angew Chem Int Ed Engl. 62: e202307157. PMID: 37450419
  11. 从化学转化的人类细胞系中分子克隆出一种新的转化基因。  |  Cooper, CS., et al. Nature. 311: 29-33. PMID: 6590967
  12. 丝氨酸 985 的磷酸化对肝细胞生长因子受体激酶有负向调节作用。  |  Gandino, L., et al. 1994. J Biol Chem. 269: 1815-20. PMID: 8294430

订购信息

产品名称产品编号规格价格数量收藏夹

Met 抗体 (B-2)

sc-8057
200 µg/ml
RMB2377.00

Met (B-2): m-IgG Fc BP-HRP 套装

sc-526513
200 µg Ab; 10 µg BP
RMB2662.00

Met (B-2): m-IgG1 BP-HRP 套装

sc-531886
200 µg Ab; 20 µg BP
RMB2662.00

Met 抗体 (B-2) AC

sc-8057 AC
500 µg/ml, 25% agarose
RMB3129.00

Met 抗体 (B-2) HRP

sc-8057 HRP
200 µg/ml
RMB2377.00

Met 抗体 (B-2) FITC

sc-8057 FITC
200 µg/ml
RMB2482.00

Met 抗体 (B-2) PE

sc-8057 PE
200 µg/ml
RMB2580.00

Met 抗体 (B-2) Alexa Fluor® 488

sc-8057 AF488
200 µg/ml
RMB2685.00

Met 抗体 (B-2) Alexa Fluor® 546

sc-8057 AF546
200 µg/ml
RMB2685.00

Met 抗体 (B-2) Alexa Fluor® 594

sc-8057 AF594
200 µg/ml
RMB2685.00

Met 抗体 (B-2) Alexa Fluor® 647

sc-8057 AF647
200 µg/ml
RMB2685.00

Met 抗体 (B-2) Alexa Fluor® 680

sc-8057 AF680
200 µg/ml
RMB2685.00

Met 抗体 (B-2) Alexa Fluor® 790

sc-8057 AF790
200 µg/ml
RMB2685.00

Met (B-2) 中和勝肽

sc-8057 P
100 µg/0.5 ml
RMB511.00

What is the recommended secondary for use with this Met Antibody (B-2) mouse monoclonal antibody?

Asked by: Anna99
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Technical Support 8
Date published: 2023-10-13
  • y_2025, m_8, d_30, h_9CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_8057, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 116ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from No signal in WBWe tested the antibody using A459 and MKN23 whole cell lysates in the following conditions: dilution 1:500 and 1:100 dilution, o.n. incubation at 4°C. No signal detected at the expected MW (150 - 135 kDa).
Date published: 2022-03-28
Rated 1 out of 5 by from No DetectionWe saw some specific banding, but no detection at right weight.
Date published: 2021-11-22
Rated 5 out of 5 by from So far so goodWorks very well for IF on mouse kidney cryosections.
Date published: 2019-04-19
Rated 2 out of 5 by from Did not work in mouse liver lysateDid not work in mouse liver lysate, I tried it with 1:500 dilutions
Date published: 2018-04-12
Rated 2 out of 5 by from Not specificThe antibody is very strong and clean but it detects a band near 30-kDa in MCF-7 cells.
Date published: 2017-09-11
Rated 5 out of 5 by from specific bandsThe specific bands were detected by Western blot and 1:500 in mouse kidney tissues.
Date published: 2017-05-24
Rated 5 out of 5 by from WB 结果很不错用鼠的内皮细胞提取蛋白,条带很特异。背景很干净。 用去石蜡切片免疫荧光效果还不清楚。过段时间再评论。
Date published: 2017-03-09
Rated 5 out of 5 by from Superb !!Outstanding western data; strong signal with low background; also great IFC staining !!
Date published: 2017-01-27
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_15
  • loc_zh_CN, sid_8057, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 6ms
  • REVIEWS, PRODUCT
Met 抗体 (B-2) is rated 4.1 out of 5 by 15.
  • y_2025, m_8, d_30, h_9
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_15
  • loc_zh_CN, sid_8057, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 74ms
  • REVIEWS, PRODUCT