Date published: 2025-9-6

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p-FAK 抗体 (A-12): sc-374668

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说明书
  • p-FAK抗体(A-12)是小鼠单克隆IgG1 κ, 在14篇文献中引用,规格为200 µg/ml
  • p-FAK 抗体 (A-12) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ,检测 mouse, rat, human 和 来源的Ser 722 phosphorylated FAK; 也和以下物种反应,包括: 和 equine, canine and avian
  • 抗p-FAK抗体(A-12)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p-FAK (A-12): sc-374668免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRPm-IgG1 BP-HRP 是 p-FAK 抗体 (A-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与p-FAK 抗体 (A-12) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

p-FAK抗体(A-12)是一种IgG1 κ小鼠单克隆p-FAK抗体,可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的Ser 722磷酸化FAK。p-FAK抗体(A-12)既可作为非共轭的抗p-FAK抗体形式提供,也可作为多种共轭形式的抗p-FAK抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。真核细胞细胞外基质(ECM)中整合素的激活促进膜粘附复合物的形成,称为粘着斑,其中可包括细胞骨架蛋白和蛋白质酪氨酸激酶,如粘着斑激酶(FAK)。粘着斑内发生的磷酸化事件影响许多过程,包括有丝分裂信号传导、细胞存活和细胞运动。FAK是一种非受体酪氨酸激酶,在物种之间普遍表达且高度保守。FAK被整合素簇招募,并根据粘着斑中存在的效应分子进行不同的磷酸化。在血清饥饿、接触抑制和细胞周期阻滞等所有条件下,FAK Tyr 397的磷酸化水平均降低,而在这些条件下,激活FAK Tyr 407的磷酸化水平则增加。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

p-FAK 抗体 (A-12)

sc-374668
200 µg/ml
RMB2377.00

p-FAK (A-12): m-IgG Fc BP-HRP 套装

sc-527498
200 µg Ab; 10 µg BP
RMB2662.00

p-FAK (A-12): m-IgG1 BP-HRP 套装

sc-532871
200 µg Ab; 20 µg BP
RMB2662.00

p-FAK 抗体 (A-12) AC

sc-374668 AC
500 µg/ml, 25% agarose
RMB3129.00

p-FAK 抗体 (A-12) HRP

sc-374668 HRP
200 µg/ml
RMB2377.00

p-FAK 抗体 (A-12) FITC

sc-374668 FITC
200 µg/ml
RMB2482.00

p-FAK 抗体 (A-12) PE

sc-374668 PE
200 µg/ml
RMB2580.00

p-FAK 抗体 (A-12) Alexa Fluor® 488

sc-374668 AF488
200 µg/ml
RMB2685.00

p-FAK 抗体 (A-12) Alexa Fluor® 546

sc-374668 AF546
200 µg/ml
RMB2685.00

p-FAK 抗体 (A-12) Alexa Fluor® 594

sc-374668 AF594
200 µg/ml
RMB2685.00

p-FAK 抗体 (A-12) Alexa Fluor® 647

sc-374668 AF647
200 µg/ml
RMB2685.00

p-FAK 抗体 (A-12) Alexa Fluor® 680

sc-374668 AF680
200 µg/ml
RMB2685.00

p-FAK 抗体 (A-12) Alexa Fluor® 790

sc-374668 AF790
200 µg/ml
RMB2685.00

p-FAK (A-12) 中和勝肽

sc-374668 P
100 µg/0.5 ml
RMB511.00

What application is the blocking peptide sc-374668 P appropriate for?

Asked by: AbPolly
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent AntibodyWorks very well for WB and IF from human podocytes, clean signal, no background.
Date published: 2020-10-01
Rated 4 out of 5 by from Works for WB, but not good for IFI got a band by WB about the right size. I tried IF too (on human cancer cells), but it didn't work (1/50 dilution).
Date published: 2019-05-14
Rated 5 out of 5 by from works great for WBThis antibody detected GFP-FAK (human) overexpressed in HEK 293T cells. Used at 1:500 dilution. Only minor background bands.
Date published: 2018-01-20
Rated 3 out of 5 by from May Have WorkedBlocked in 5% BSA-TBST 1:500. Incubated in primary overnight in cold room. Tested on Murine Fallopian Tube Tissue and Human Ovarian Cell Lines. There were strong bands at 50 KD.
Date published: 2017-07-24
Rated 5 out of 5 by from Decent western blot detectionThis antibody showed a decent sensitivity in western blot. For me, to detect a phosphorylation of FAK upon a drug treatment and this sample showed some difference between the drug and control in 1: 500 dilution O/N.
Date published: 2017-06-29
Rated 5 out of 5 by from Funcks very good!This antibody works well in our study, background is very clear.
Date published: 2017-06-25
Rated 5 out of 5 by from Perfect productI used this Ab to checked the MCF-7 cells expression. The band is so clear
Date published: 2017-05-12
Rated 5 out of 5 by from Good antibody for WBI got very clean blot with this antibody, I used it on human samples, BSA as the blocking buffer and the dilution was 1:1000
Date published: 2017-03-30
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p-FAK 抗体 (A-12) is rated 4.7 out of 5 by 12.
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