Date published: 2025-9-6
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FAK Antibody (H-1) - Western Blotting - Image 2152
FAK 抗体 (H-1): sc-1688. 对 HeLa 全细胞裂解液中 FAK 的表达进行 Western 印迹分析.
FAK 抗体 (H-1): sc-1688. 对 HeLa 全细胞裂解液中 FAK 的表达进行 Western 印迹分析.

FAK 抗体 (H-1): sc-1688

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说明书
  • FAK抗体(H-1)是小鼠单克隆IgG1 κ, 在111篇文献中引用，规格为200 µg/ml
  • 免疫mouse物种的FAK的氨基酸903-1052
  • 抗-FAK 抗体 (H-1) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 FAK p125
  • FAK 抗体 (H-1) 可偶联 藻红蛋白 用于 IF, IHC(P) 和 FCM
  • 还可偶联Alexa Fluor® 594 用于IF, IHC(P) 和 FCM
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取FAK (H-1): sc-1688的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 FAK 抗体 (H-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与FAK 抗体 (H-1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

FAK抗体（H-1）是一种IgG1 κ小鼠单克隆FAK抗体，可通过WB、IP、IF、IHC（P）和FCM检测来自小鼠、大鼠和人类的FAK p125。FAK抗体（H-1）既可以是偶联的，也可以是非偶联的抗FAK抗体形式。焦点黏附激酶最初被识别为Src编码的pp60的固有蛋白酪氨酸激酶活性的主要底物。FAK p125的推断氨基酸序列表明它是一种细胞质蛋白酪氨酸激酶，其序列和结构组织与迄今为止描述的其他蛋白质相比是独一无二的。通过免疫荧光定位p125表明它主要存在于细胞焦点黏附，因此被称为焦点黏附激酶（FAK）。FAK集中在正在活动迁移和快速增殖修复烧伤伤口的基底角质细胞的基底边缘，并在培养基中扩展的角质细胞的焦点黏附中被激活和定位。因此，推测FAK可能在人类伤口的再上皮化中具有重要的体内作用。也已经证明，FAK蛋白酪氨酸激酶活性也会增加，以通过G蛋白偶联受体作用的促生长神经肽或神经递质刺激的细胞。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

FAK 抗体 (H-1) 参考文献:

  1. 转化 G 蛋白偶联受体的激活会诱导细胞蛋白快速发生酪氨酸磷酸化，包括 p125FAK 和 p130 v-src 底物。  |  Gutkind, JS. and Robbins, KC. 1992. Biochem Biophys Res Commun. 188: 155-61. PMID: 1329743
  2. 细胞粘附和致癌转化对局灶粘附相关蛋白酪氨酸激酶的调控。  |  Guan, JL. and Shalloway, D. 1992. Nature. 358: 690-2. PMID: 1379699
  3. 蚕豆素, 血管加压素和内皮素对瑞士 3T3 细胞中酪氨酸磷酸化的刺激。确定一种新型酪氨酸激酶为主要底物。  |  Zachary, I., et al. 1992. J Biol Chem. 267: 19031-4. PMID: 1382065
  4. 血小板中依赖于整合素的磷酸化和激活蛋白酪氨酸激酶 pp125FAK。  |  Lipfert, L., et al. 1992. J Cell Biol. 119: 905-12. PMID: 1385445
  5. 在细胞附着到纤维粘连蛋白时磷酸化的病灶粘附蛋白-酪氨酸激酶。  |  Hanks, SK., et al. 1992. Proc Natl Acad Sci U S A. 89: 8487-91. PMID: 1528852
  6. pp125FAK是一种与病灶粘连有关的结构独特的蛋白酪氨酸激酶。  |  Schaller, MD., et al. 1992. Proc Natl Acad Sci U S A. 89: 5192-6. PMID: 1594631
  7. 焦点粘附激酶 pp125FAK 的自身磷酸化可引导 pp60src 与 SH2 依赖性结合。  |  Schaller, MD., et al. 1994. Mol Cell Biol. 14: 1680-8. PMID: 7509446
  8. 局灶粘附激酶在表皮伤口附近和培养过程中迁移和增殖的角质细胞中的潜在作用。  |  Gates, RE., et al. 1994. Cell Growth Differ. 5: 891-9. PMID: 7986754

订购信息

产品名称产品编号规格价格数量收藏夹

FAK 抗体 (H-1)

sc-1688
200 µg/ml
RMB2377.00

FAK (H-1): m-IgG Fc BP-HRP 套装

sc-526472
200 µg Ab; 10 µg BP
RMB2662.00

FAK (H-1): m-IgG1 BP-HRP 套装

sc-531845
200 µg Ab; 20 µg BP
RMB2662.00

FAK 抗体 (H-1) PE

sc-1688 PE
200 µg/ml
RMB2580.00

FAK 抗体 (H-1) Alexa Fluor® 546

sc-1688 AF546
200 µg/ml
RMB2685.00

FAK 抗体 (H-1) Alexa Fluor® 594

sc-1688 AF594
200 µg/ml
RMB2685.00

FAK 抗体 (H-1) Alexa Fluor® 680

sc-1688 AF680
200 µg/ml
RMB2685.00

FAK 抗体 (H-1) Alexa Fluor® 790

sc-1688 AF790
200 µg/ml
RMB2685.00

Hello, I am using Anti Fak SC-1688 in WB, but I see another bands in 250, 75 and 50 kD. Why is it recognizing other proteins?

Asked by: marithearceo
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2019-01-29

Can I ask for a quote for the product----FAK Antibody (H-1): sc-1688 PE? Thanks.

Asked by: Jen Ming
Thank you for your question. Our pricing in Australian Dollars (AUD) for this PE conjugated antibody is available on our website. You can change the currency and language settings on our website by going to the top of the page and using the drop-down menus. Please contact our Customer Care Department to request a formal quote. Or you can contact one of our authorized distributors in Australia. Please use the link below to find all of the contact information to request a quote, or place an order. https://www.scbt.com/scbt/resources/distributors
Answered by: Technical Support
Date published: 2017-07-31

I am planning on performing some IHC. Do you have recommendations for doing this will only mouse monoclonal antibodies?

Asked by: Cweed
We suggest using directly conjugated primary antibodies. For immunostaining, consider using sc-271126 which is available conjugated to FITC, AF647, and AF488.
Answered by: Technical Support 3
Date published: 2017-01-11

I am studying bovine samples. Do you think sc-1688 will work for my experiment?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 3
Date published: 2017-01-11
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Rated 4 out of 5 by from Work for Western blotUsing hASMC extract, detect a band in 120 kD and a stronger band in 50 kD.
Date published: 2017-11-12
Rated 5 out of 5 by from Great monoclonalStrong western blot and IHC data; low background !!
Date published: 2017-01-13
Rated 5 out of 5 by from Good for multiple applicationsGood antibody for flow cytometry and immunohistochemistry. Clear bands in Western blots as well, and the antibody is well-cited.
Date published: 2016-12-20
Rated 3 out of 5 by from Very good specificity but very low sensitivityVery good specificity but very low sensitivity for WB with samples from a rat cell line.
Date published: 2016-06-28
Rated 5 out of 5 by from This has worked well in WBThis has worked well in WB.
Date published: 2015-10-22
Rated 5 out of 5 by from Publishable data in IHC(P) using breast tumorPublishable data in IHC(P) using breast tumor, cervical epithelium and cervical carcinoma. -SCBT Publication Review
Date published: 2015-05-19
Rated 4 out of 5 by from Worked with transfected lysate by WBWorked with transfected lysate by WB -SCBT QC
Date published: 2015-03-17
Rated 4 out of 5 by from Robust band detected in WB using HeLa wholeRobust band detected in WB using HeLa whole cell lysate. -SCBT QC
Date published: 2015-01-29
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FAK 抗体 (H-1) is rated 4.5 out of 5 by 12.
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