Date published: 2025-9-6
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p-FAK Antibody (2D11) - Western Blotting - Image 38523
p-FAK 抗体 (2D11): sc-81493. 非转染: sc-117752 (A) 和人 FAK 转染: sc-114600 (B) 293T 全细胞裂解液中 FAK 磷酸化的 Western 印迹分析.
p-FAK Antibody (2D11) - Western Blotting - Image 38523
p-FAK 抗体 (2D11): sc-81493. 非转染: sc-117752 (A) 和人 FAK 转染: sc-114600 (B) 293T 全细胞裂解液中 FAK 磷酸化的 Western 印迹分析.
p-FAK Antibody (2D11) - Western Blotting - Image 62523
p-FAK 抗体 (2D11): sc-81493. 对 HeLa 全细胞裂解液中的 FAK 磷酸化进行 Western 印迹分析.
p-FAK 抗体 (2D11): sc-81493. 非转染: sc-117752 (A) 和人 FAK 转染: sc-114600 (B) 293T 全细胞裂解液中 FAK 磷酸化的 Western 印迹分析.

p-FAK 抗体 (2D11): sc-81493

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寫評論提問

说明书
  • p-FAK抗体(2D11)是小鼠单克隆IgG1 κ, 在61篇文献中引用，规格为50 µg/0.5 ml
  • 针对磷酸化肽段，与FAK的酪氨酸397周围的氨基酸残基对应，human来源
  • 推荐用于 mouse, rat, human 和 canine 来源的Tyr 397 phosphorylated FAK WB 和 IP检测
  • 关于如何获取p-FAK (2D11): sc-81493的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是p-FAK Antibody (2D11) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与p-FAK Antibody (2D11) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

p-FAK抗体（2D11）是一种IgG1 κ小鼠单克隆p-FAK抗体，可通过WB和IP检测小鼠、大鼠和人类来源的Tyr 397磷酸化FAK。p-FAK抗体（2D11）以非共轭的抗p-FAK抗体形式提供。真核细胞细胞外基质（ECM）中整合素的激活促进膜粘附复合物的形成，称为粘着斑，其中可能包括细胞骨架蛋白和蛋白质酪氨酸激酶，如粘着斑激酶（FAK）。粘着斑内发生的磷酸化事件影响许多过程，包括有丝分裂信号传导、细胞存活和细胞运动。FAK是一种非受体酪氨酸激酶，在物种之间普遍表达且高度保守。FAK被整合素簇招募，并根据粘着斑中存在的效应分子进行不同的磷酸化。在血清饥饿、接触抑制和细胞周期阻滞等所有条件下，FAK Tyr 397的磷酸化水平均降低，而在这些条件下，激活FAK Tyr 407的磷酸化水平则增加。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-FAK 抗体 (2D11) 参考文献:

  1. 细胞粘附和致癌转化对局灶粘附相关蛋白酪氨酸激酶的调控。  |  Guan, JL. and Shalloway, D. 1992. Nature. 358: 690-2. PMID: 1379699
  2. 血小板中依赖于整合素的磷酸化和激活蛋白酪氨酸激酶 pp125FAK。  |  Lipfert, L., et al. 1992. J Cell Biol. 119: 905-12. PMID: 1385445
  3. 在细胞附着到纤维粘连蛋白时磷酸化的病灶粘附蛋白-酪氨酸激酶。  |  Hanks, SK., et al. 1992. Proc Natl Acad Sci U S A. 89: 8487-91. PMID: 1528852
  4. pp125FAK是一种与病灶粘连有关的结构独特的蛋白酪氨酸激酶。  |  Schaller, MD., et al. 1992. Proc Natl Acad Sci U S A. 89: 5192-6. PMID: 1594631
  5. 病灶粘附激酶受酪氨酸 407 磷酸化的负调控。  |  Lim, Y., et al. 2007. J Biol Chem. 282: 10398-404. PMID: 17303567
  6. mTORC1/rpS6和p-FAK-Y407信号传导调节精子发生：从adudin药物/毒物模型研究中获得的见解。  |  Wang, L., et al. 2022. Semin Cell Dev Biol. 121: 53-62. PMID: 33867214
  7. 磷酸化的 paxillin 和磷酸化的 FAK 构成了病灶粘附的亚区域。  |  Bachmann, M., et al. 2022. J Cell Sci. 135: PMID: 35343568
  8. 癌相关成纤维细胞衍生的富含赖氨酰氧化酶的细胞外囊泡通过p-FAK/paxillin/YAP信号传导介导胶原交联并促进上皮-间质转化  |  Liu, X., et al. 2023. Int J Oral Sci. 15: 32. PMID: 37532712
  9. 焦点粘附激酶 pp125FAK 的自身磷酸化可引导 pp60src 与 SH2 依赖性结合。  |  Schaller, MD., et al. 1994. Mol Cell Biol. 14: 1680-8. PMID: 7509446
  10. Src家族蛋白酪氨酸激酶对焦点粘附激酶上的Grb2 SH2域结合位点进行体内磷酸化的证据。  |  Schlaepfer, DD. and Hunter, T. 1996. Mol Cell Biol. 16: 5623-33. PMID: 8816475

订购信息

产品名称产品编号规格价格数量收藏夹

p-FAK 抗体 (2D11)

sc-81493
50 µg/0.5 ml
RMB2377.00

p-FAK (2D11): m-IgGκ BP-HRP 套装

sc-534469
50 µg Ab; 40 µg BP
RMB2662.00

p-FAK (2D11): m-IgG1 BP-HRP 套装

sc-538867
50 µg Ab; 20 µg BP
RMB2662.00

How the dilution of this antibody should be prepared for use in western blotting

Asked by: Anonymous
Thank you for your question. p-FAK (2D11) is recommended for detection of Tyr 397 phosphorylated FAK of mouse, rat, human and canine origin by Western Blotting with a starting dilution of 1:200, and a dilution range of 1:100-1:1000.
Answered by: Tech Support Europe
Date published: 2025-01-21

What is the best secondary antibody for this mouse monoclonal?

Asked by: Anna99
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-13

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with p-FAK Antibody (2D11): sc-81493 ?

Asked by: Anna99
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Service 11
Date published: 2017-01-13
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Rated 4 out of 5 by from Works well in WBThis antibody works well in WB, I used it to detect p-FAK in Huh7, dil:1:500
Date published: 2020-02-21
Rated 1 out of 5 by from Not working antibodyI recived a free sample from this antibody and it din´t work. I am working with MDCK cells also I charge a sample from HeLa as a positive control, I used 1:50, 1:200 and 1:500 dilution and in any of those I saw a band on the western blot. I used GAPDH as a charge control and I tested my secondary antibody as well with no failures.
Date published: 2019-07-22
Rated 2 out of 5 by from Non-specific bands obscure resultsI tried this on lysates from both mouse and humans cells in experiments where I was blotting for several other proteins at the same time. The others worked, but there was a strong band too large to be P-FAK that made it very difficult to get a result. The FAK (B-8) antibody worked to confirm that this larger band was not FAK. I suppose you could get a result if you ran a low % gel for a long time to get more separation.
Date published: 2019-01-18
Rated 5 out of 5 by from good band in WBWorks good in WB with HCECs human corneal epithelial cells .
Date published: 2018-11-22
Rated 5 out of 5 by from Nice band in WBp-FAK had strong band in WB in human leukemia cell line Molt4
Date published: 2018-07-09
Rated 5 out of 5 by from Good for WB in cell lysatesWB using p-FAK (2D11) 1:1000 in 76NE6 cells, clear band detected.
Date published: 2018-05-21
Rated 5 out of 5 by from Fantastic for Western BlotUsed this antibody at 1:1000 dilution on a western blot with 100 ug of protein from tissue and another blot with 60 ug of protein from cancer cell lines. Both blots came out absolutely crystal clear.
Date published: 2018-02-21
Rated 5 out of 5 by from Good antibody!1:500 diluted for wb of rat protein,the band is quite strong and special!
Date published: 2017-12-30
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p-FAK 抗体 (2D11) is rated 4.1 out of 5 by 23.
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