Date published: 2025-9-7
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p-beta-catenin Antibody (BC-22) - Western Blotting - Image 34784
p-β-catenin (BC-22): sc-57535. 未转染: sc-117752 (A) 和转染人 p-β-catenin: sc-116622 (B) 的 293T 全细胞裂解液中 p-β-catenin 磷酸化的 Western 印迹分析.
p-beta-catenin Antibody (BC-22) - Western Blotting - Image 34784
p-β-catenin (BC-22): sc-57535. 未转染: sc-117752 (A) 和转染人 p-β-catenin: sc-116622 (B) 的 293T 全细胞裂解液中 p-β-catenin 磷酸化的 Western 印迹分析.
p-beta-catenin Antibody (BC-22) - Western Blotting - Image 370448
p-β-catenin 抗体 (BC-22): sc-57535. 在 OVCAR-3 + pervanadate (A), SH-SY5Y + calyculin (B) 和 293T + calyculin (C) 全细胞裂解液中 p-β-catenin 表达的 Western 印迹分析.
p-β-catenin (BC-22): sc-57535. 未转染: sc-117752 (A) 和转染人 p-β-catenin: sc-116622 (B) 的 293T 全细胞裂解液中 p-β-catenin 磷酸化的 Western 印迹分析.

p-beta-catenin 抗体 (BC-22): sc-57535

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寫評論提問

说明书
  • p-beta-catenin 抗体 BC-22 是小鼠单克隆 IgG2b κ，p-beta-catenin抗体, 在31篇文献中引用，规格为50 µg/0.5 ml
  • 免疫human物种的β-catenin的氨基酸Ser 33/37
  • 推荐用于 mouse, rat 和 human 来源的Ser 33/Ser 37 phosphorylated β-catenin WB, IP 和 IF检测; 不建议用于检测the unphosphorylated or the Ser33 phosphorylated protein or phosphorylated plakoglobin (despite the high homology in the phosphorylation site with β-catenin)
  • 关于如何获取p-beta-catenin (BC-22): sc-57535的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 2b BP-HRP">m-IgG2b BP-HRP是p-beta-catenin Antibody (BC-22) 适用于 WB 应用。 的首选辅助检测试剂，这些试剂现与p-beta-catenin Antibody (BC-22) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加Catenin抗体，包括α E-catenin, α T-catenin, β-catenin, δ-catenin和γ-catenin

p-β-catenin 抗体（BC-22）是一种小鼠单克隆 IgG2b kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）检测小鼠、大鼠和人类来源的 p-β-catenin 蛋白。抗 p-β-catenin 抗体（BC-22）以非 非结合形式。p-β-catenin蛋白，也称为β-Catenin或CTNNB1，在Wnt信号传导通路中起着关键作用，该通路可调节细胞增殖、分化及迁移。p-β-catenin蛋白通过与E-cadherin相互作用形成catenin/cadherin复合物，从而维持细胞粘附和组织完整性，该复合物可介导细胞-细胞粘附。p-β-catenin蛋白失调会导致信号异常，与多种癌症有关，包括结肠癌和乳腺癌。p-β-catenin蛋白主要定位于细胞质和细胞核，在细胞质和细胞核中，p-β-catenin蛋白充当Wnt靶基因的转录共激活因子。p-β-catenin蛋白的核转运受到严格调控，核积累可能是由翻译后修饰引起的，例如糖原合酶激酶-3β的磷酸化。这些修饰会影响p-β-catenin蛋白的稳定性以及与其他蛋白的相互作用，包括肿瘤抑制剂APC和调节剂Pin1，它们可以进一步调节信号通路。了解p-β-catenin蛋白的定位和功能对于阐明其在肿瘤发生中的作用和开发靶向疗法至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-beta-catenin 抗体 (BC-22) 参考文献:

  1. 人类子宫内膜癌中 CTNNB1（β-catenin）基因的突变分析：密码子 34 处的频繁突变会导致核蓄积。  |  Ikeda, T., et al. 2000. Oncol Rep. 7: 323-6. PMID: 10671680
  2. Pin1 通过抑制 beta-catenin 与 APC 的相互作用来调节其周转和亚细胞定位。  |  Ryo, A., et al. 2001. Nat Cell Biol. 3: 793-801. PMID: 11533658
  3. 正常细胞和转化细胞中S33/S37磷酸化β-catenin的调节。  |  Sadot, E., et al. 2002. J Cell Sci. 115: 2771-80. PMID: 12077367
  4. CK2 对 beta-catenin 的犰狳重复区域的磷酸化可增强 Wnt 信号转导。  |  Song, DH., et al. 2003. J Biol Chem. 278: 24018-25. PMID: 12700239
  5. 磷酸化β-catenin与E-cadherin在细胞接触处以及APC在细胞突起处的独立相互作用。  |  Faux, MC., et al. 2010. PLoS One. 5: e14127. PMID: 21152425
  6. 选择性14-3-3γ诱导在脑皮质神经元缺血期间熄灭p-β-catenin Ser37/Bax增强细胞死亡。  |  Lai, XJ., et al. 2014. Cell Death Dis. 5: e1184. PMID: 24743739
  7. 在牛疱疹病毒 1 对 MDBK 细胞的生产性感染过程中，磷脂酶 C-γ1 有可能促进活化的 β-catenin（p-β-catenin(S552)）的亚细胞定位。  |  Liu, C., et al. 2023. Vet Microbiol. 276: 109626. PMID: 36502739
  8. 人类乳腺癌细胞系中 E-cadherin, α-和β-catenin 表达的频繁变化。  |  Pierceall, WE., et al. 1995. Oncogene. 11: 1319-26. PMID: 7478552
  9. E-cadherin/alpha-catenin 复合物在调节侵袭性结肠癌细胞的细胞-细胞和细胞-基质粘附特性中的作用。  |  Breen, E., et al. 1995. Ann Surg Oncol. 2: 378-85. PMID: 7496831
  10. α-肌动蛋白通过α-卡替宁与粘连蛋白/卡替宁细胞-细胞粘附复合体相互作用。  |  Knudsen, KA., et al. 1995. J Cell Biol. 130: 67-77. PMID: 7790378

订购信息

产品名称产品编号规格价格数量收藏夹

p-beta-catenin 抗体 (BC-22)

sc-57535
50 µg/0.5 ml
RMB2377.00

p-beta-catenin (BC-22): m-IgG Fc BP-HRP 套装

sc-539058
50 µg Ab; 10 µg BP
RMB2662.00

p-beta-catenin (BC-22): m-IgG2b BP-HRP 套装

sc-549730
50 µg Ab; 10 µg BP
RMB2662.00

I am using p-β-catenin (BC-22): sc-57535 for immunofluorescence, what dilution of this antibody should I use?

Asked by: jerojero
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 2 out of 5 by from Thanks for the sample.A very large number of bands other than the target band were detected. Therefore, it is difficult to see the target band.
Date published: 2023-09-28
Rated 3 out of 5 by from decentDecent antibody. used at 1:1000 on 15ug of bone homogenates from mice femurs.
Date published: 2018-05-22
Rated 4 out of 5 by from Works well for mouse samplesWorks at 1:200 dilution in WB application. However, needed longer exposure times.
Date published: 2018-03-22
Rated 1 out of 5 by from nothing detectedthe antibody was tested in western blot on rat tissue samples and did not give any band in my hands
Date published: 2017-12-13
Rated 1 out of 5 by from non-specificthe phosphor beta catenin (SER33/37) I have used and it was very nonspecific. I received multiple banding across the entire lane of each well. I couldn’t determine the true band for my desired protein.
Date published: 2017-11-09
Rated 1 out of 5 by from Not a good antibodyI tried this antibody on different cell lines. I started with 1:1000 dilution, and saw nothing. Then I tried 1:500, still nothing. I finally tried 1:250, and after a long exposure I detected a very faint band at the right size of phospho-beta-catenin with a lot of background. Overall, not a good experience with this antibody.
Date published: 2017-10-24
Rated 2 out of 5 by from Not very goodIt is hard to get a clear band in Western Blot. Tried at a concentration of 1:1000 in western blot.
Date published: 2017-10-10
Rated 3 out of 5 by from Inclusive resultsThis antibody was tested on human aortic endothelial cells post various treatments. It gives a band around 95 kDa with some background. The specific band was confirmed by blotting the same membrane with total beta-catenin
Date published: 2017-09-14
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p-beta-catenin 抗体 (BC-22) is rated 2.9 out of 5 by 20.
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