Date published: 2025-9-7
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beta-catenin Antibody (E-5) - Western Blotting - Image 390712
β-catenin 抗体 (E-5) Alexa Fluor® 594: sc-7963 AF594. C3H/10T1/2 (A), PC-12 (B), A-431 (C), SH-SY5Y (D), MCF7 (E) 和 IMR-32 (F) 全细胞裂解液中 β-catenin 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker MW Tag-Alexa Fluor® 790 检测 Cruz Marker™ 分子量标准: sc-516731.
β-catenin 抗体 (E-5) Alexa Fluor® 594: sc-7963 AF594. C3H/10T1/2 (A), PC-12 (B), A-431 (C), SH-SY5Y (D), MCF7 (E) 和 IMR-32 (F) 全细胞裂解液中 β-catenin 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker MW Tag-Alexa Fluor® 790 检测 Cruz Marker™ 分子量标准: sc-516731.

beta-catenin 抗体 (E-5): sc-7963

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寫評論提問

说明书
  • beta-catenin 抗体 E-5 是小鼠单克隆 IgG1 κ，beta-catenin抗体, 在1,001篇文献中引用，规格为200 µg/ml
  • 免疫human来源β-catenin位于C-terminus 的680-781氨基酸
  • beta-catenin 抗体 (E-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 β-catenin
  • 抗beta-catenin抗体（E-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取beta-catenin (E-5): sc-7963的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 beta-catenin 抗体 (E-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与beta-catenin 抗体 (E-5) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Catenin抗体，包括α E-catenin, α T-catenin, β-catenin, δ-catenin和γ-catenin

β-catenin抗体（E-5）是一种小鼠单克隆IgG1 kappa轻链抗体，专门针对人类β-catenin C端680-781氨基酸。β-catenin 单克隆抗体（E-5）可有效检测小鼠、大鼠和人类来源的β-catenin，适用于多种应用，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）。抗-β-catenin 抗体（E-5）有非结合型和多种结合型，如琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种 Alexa Fluor® 结合物，可为各种实验需求提供多种解决方案。β-Catenin，也称为catenin beta-1或CTNNB1，是一种多功能蛋白，是细胞粘附和基因转录调控不可或缺的组成部分。β-catenin与包括E-cadherin在内的细胞粘附蛋白的细胞质尾部结合，形成维持细胞粘附和组织结构所必需的catenin-cadherin复合物。除了结构作用外，β-catenin还是Wnt信号传导通路的关键组成部分，其在细胞质中的积累以及随后向细胞核的转移会影响细胞增殖和分化相关基因的转录。β-catenin失调与多种癌症有关，这凸显了其在细胞信号传导和粘附中的重要性。β-catenin单克隆抗体（E-5）是研究人员研究这些关键细胞过程不可或缺的工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

beta-catenin 抗体 (E-5) 参考文献:

  1. β-catenin的多面性和功能。  |  Valenta, T., et al. 2012. EMBO J. 31: 2714-36. PMID: 22617422
  2. 在 Wnt 信号通路中直接靶向 β-catenin： 目前的进展和前景  |  Wang, Z., et al. 2021. Med Res Rev. 41: 2109-2129. PMID: 33475177
  3. Wnt/β-catenin信号抑制剂。  |  Zhang, X., et al. 2023. Curr Top Med Chem. 23: 880-896. PMID: 36872364
  4. USP8 通过稳定 β-catenin 积极调控肝细胞癌的肿瘤发生并赋予其抗铁锈色素沉着的能力  |  Tang, J., et al. 2023. Cell Death Dis. 14: 360. PMID: 37311739
  5. β-catenin的遗传失活是有益的，而在去瘤素心肌病中其激活则是有害的。  |  Olcum, M., et al. 2023. Cardiovasc Res. 119: 2712-2728. PMID: 37625794
  6. 人类乳腺癌细胞系中 E-cadherin, α-和β-catenin 表达的频繁变化。  |  Pierceall, WE., et al. 1995. Oncogene. 11: 1319-26. PMID: 7478552
  7. E-cadherin/alpha-catenin 复合物在调节侵袭性结肠癌细胞的细胞-细胞和细胞-基质粘附特性中的作用。  |  Breen, E., et al. 1995. Ann Surg Oncol. 2: 378-85. PMID: 7496831
  8. 受体蛋白酪氨酸磷酸酶 PTPmu 在体内与粘附蛋白和 catenins 相关联。  |  Brady-Kalnay, SM., et al. 1995. J Cell Biol. 130: 977-86. PMID: 7642713
  9. 鉴定与 N-粘连蛋白和 alpha-catenin 结合所需的 plakoglobin 结构域。  |  Sacco, PA., et al. 1995. J Biol Chem. 270: 20201-6. PMID: 7650039
  10. α-肌动蛋白通过α-卡替宁与粘连蛋白/卡替宁细胞-细胞粘附复合体相互作用。  |  Knudsen, KA., et al. 1995. J Cell Biol. 130: 67-77. PMID: 7790378
  11. β-catenin在人类癌症中的表达。  |  Takayama, T., et al. 1996. Am J Pathol. 148: 39-46. PMID: 8546224
  12. 克隆缺乏第四个犰狳重复的 plakoglobin（γ-catenin）的替代形式。  |  Ozawa, M., et al. 1995. J Biochem. 118: 836-40. PMID: 8576101

订购信息

产品名称产品编号规格价格数量收藏夹

beta-catenin 抗体 (E-5)

sc-7963
200 µg/ml
RMB2377.00

beta-catenin (E-5): m-IgG Fc BP-HRP 套装

sc-528188
200 µg Ab; 10 µg BP
RMB2662.00

beta-catenin (E-5): m-IgGκ BP-HRP 套装

sc-520510
200 µg Ab, 40 µg BP
RMB2662.00

beta-catenin (E-5): m-IgG1 BP-HRP 套装

sc-542807
200 µg Ab; 20 µg BP
RMB2662.00

beta-catenin 抗体 (E-5) AC

sc-7963 AC
500 µg/ml, 25% agarose
RMB3129.00

beta-catenin 抗体 (E-5) HRP

sc-7963 HRP
200 µg/ml
RMB2377.00

beta-catenin 抗体 (E-5) FITC

sc-7963 FITC
200 µg/ml
RMB2482.00

beta-catenin 抗体 (E-5) PE

sc-7963 PE
200 µg/ml
RMB2580.00

beta-catenin 抗体 (E-5) Alexa Fluor® 488

sc-7963 AF488
200 µg/ml
RMB2685.00

beta-catenin 抗体 (E-5) Alexa Fluor® 546

sc-7963 AF546
200 µg/ml
RMB2685.00

beta-catenin 抗体 (E-5) Alexa Fluor® 594

sc-7963 AF594
200 µg/ml
RMB2685.00

beta-catenin 抗体 (E-5) Alexa Fluor® 647

sc-7963 AF647
200 µg/ml
RMB2685.00

beta-catenin 抗体 (E-5) Alexa Fluor® 680

sc-7963 AF680
200 µg/ml
RMB2685.00

beta-catenin 抗体 (E-5) Alexa Fluor® 790

sc-7963 AF790
200 µg/ml
RMB2685.00

Can we get a sample to try whether it is working in our system?

Asked by: Barun
Thank you for contacting Santa Cruz Biotechnology. Please email technical service at [email protected] for how to receive an antibody sample.
Answered by: BlakeJ
Date published: 2024-11-05

What’s the recommendation dilation for WB??

Asked by: Mayaar
Thank you for your question. We recommend 1:100-1:1000. https://www.scbt.com/resources/protocols/western-immuno-blotting
Answered by: BlakeJ
Date published: 2024-03-28

这款产品测的是磷酸化和非磷酸化的β-catenin的总和吗?有哪一款产品可以直接检测非磷酸化的β-catenin吗?

Asked by: 醫醫123
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2023-11-02

What is the suitable antigen retrieval method for this antibody?

Asked by: DishaV
Thank you for your question. You can find our recommended protocol with antigen retrieval methods under this link: https://www.scbt.com/resources/protocols/immunoperoxidase-staining
Answered by: Tech Support Europe
Date published: 2021-04-28

How much is the volume of "β-catenin Antibody (E-5) sc-7963" ?

Asked by: Farideh1
Thank you for your question. All of our normal antibodies have a total volume of 1mL.
Answered by: Tech Service 1
Date published: 2021-02-02

请问，二抗的比例范围是多少？

Asked by: 湖医学子
感谢您的提问。二抗的稀释范围是和您具体的实验方法相关的，产品主页可获取说明书，说明书中有详细的针对不同实验方法的稀释比例范围。 Thank you for your inquiry. The dilution range depend on what application used. You can get more detailed dilution information on its datasheet.
Answered by: Technical Support 8
Date published: 2018-12-21

I need to detect this protein in porcine samples. Do you know if this mouse monoclonal antibody will work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: TechService7
Date published: 2016-12-14

What is the recommended secondary reagent to be used with this mouse monoclonal primary for Western blot?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-14
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Rated 5 out of 5 by from Beta catenin IFA works well on IEC-6 cellsI used the antibody at 1:100 and it worked very well!
Date published: 2023-02-07
Rated 5 out of 5 by from works great for western blot.Strong and clear signal at the expected molecular weight
Date published: 2022-07-19
Rated 1 out of 5 by from Not goodI used the primary antibody 1:50 by IF on epithelial renal cells. Unfortunately, the signal was not detectable.
Date published: 2022-02-08
Rated 5 out of 5 by from 很好用，性价比很高我买来用来做IP实验，该抗体效价很高，性价比比其他公司高很多，可以使用很多次。WB实验用1:1000也有较好的特异性。
Date published: 2021-12-17
Rated 5 out of 5 by from 轻易就能获得漂亮的结果无论是做IHC还是WB都能获得满意的结果，抗体物美价廉，能够很好的满足实验求，少走弯路，希望以后会有更多好的产品！
Date published: 2021-03-15
Rated 5 out of 5 by from 美しいバンドが検出されます。WBで使用しました。シャープなバンドが検出されました。非特異的なバンドは一切ありません。バンドの大きさもデータシートと同じ位置です。
Date published: 2020-12-01
Rated 4 out of 5 by from The antibody works well in immunofluorescenceThe antibody performs in a good way in immunofluorescence (1:200) on mesenchymal stem cells fixed with 4% PFA
Date published: 2020-11-10
Rated 5 out of 5 by from VERY GOOD AND STABLE FOR IFWe have brought this antibody twice. It works well for both IF, IP and WB.
Date published: 2020-09-11
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beta-catenin 抗体 (E-5) is rated 4.8 out of 5 by 105.
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