Date published: 2025-9-7

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Occludin 抗体 (F-7): sc-271842

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说明书
  • Occludin抗体(F-7)是小鼠单克隆IgG2b κ, 在26篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于human物种的Occludin的N-terminus的氨基酸2-27之间
  • 抗-Occludin 抗体 (F-7) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Occludin; 也和以下物种反应,包括: 和 equine and porcine
  • 抗Occludin抗体(F-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Occludin (F-7): sc-271842免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 2b BP-HRP">m-IgG2b BP-HRP是Occludin Antibody (F-7) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与Occludin Antibody (F-7) 打包提供(请参阅下面的订购信息)。

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関連項目

Occludin 抗体 (F-7) 是一种 IgG2b κ 小鼠单克隆 Occludin 抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类的 Occludin。Occludin 抗体 (F-7) 既可以以非偶联的抗 Occludin 抗体形式存在,也可以有多种偶联形式的抗 Occludin 抗体,包括琼脂糖偶联、辣根过氧化物酶(HRP)偶联、藻红蛋白(PE)偶联、异硫氰酸荧光素(FITC)偶联和多种 Alexa Fluor® 偶联物。Occludin 是一种与上皮和内皮细胞的紧密连接密切相关的完整膜蛋白。在上皮和内皮细胞的紧密连接(TJ)结构中,Occludin 是一种四次跨膜完整膜蛋白,可以包含两个细胞外环。该蛋白质以多种磷酸化形式存在,磷酸化参与调节 Occludin 的定位和功能。Occludin 的表达受多不饱和脂肪酸的上调,从而增加跨内皮细胞的阻力,降低细胞对大分子的通透性。Occludin 的水平因组织而异;在脑组织中,Occludin 在细胞-细胞接触位点高度表达。非神经组织表达较低且分布不连续。上皮 Occludin 的上调可能在增强细胞旁通透性方面发挥作用,并可能与紧密连接的损伤有关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Occludin 抗体 (F-7)

sc-271842
200 µg/ml
RMB2377.00

Occludin (F-7): m-IgG2b BP-HRP 套装

sc-548709
200 µg Ab; 10 µg BP
RMB2662.00

Occludin 抗体 (F-7) AC

sc-271842 AC
500 µg/ml, 25% agarose
RMB3129.00

Occludin 抗体 (F-7) HRP

sc-271842 HRP
200 µg/ml
RMB2377.00

Occludin 抗体 (F-7) FITC

sc-271842 FITC
200 µg/ml
RMB2482.00

Occludin 抗体 (F-7) PE

sc-271842 PE
200 µg/ml
RMB2580.00

Occludin 抗体 (F-7) Alexa Fluor® 488

sc-271842 AF488
200 µg/ml
RMB2685.00

Occludin 抗体 (F-7) Alexa Fluor® 546

sc-271842 AF546
200 µg/ml
RMB2685.00

Occludin 抗体 (F-7) Alexa Fluor® 594

sc-271842 AF594
200 µg/ml
RMB2685.00

Occludin 抗体 (F-7) Alexa Fluor® 647

sc-271842 AF647
200 µg/ml
RMB2685.00

Occludin 抗体 (F-7) Alexa Fluor® 680

sc-271842 AF680
200 µg/ml
RMB2685.00

Occludin 抗体 (F-7) Alexa Fluor® 790

sc-271842 AF790
200 µg/ml
RMB2685.00

Occludin (F-7) 中和勝肽

sc-271842 P
100 µg/0.5 ml
RMB511.00

该抗体偶联Alexa Fluor® 488,Alexa Fluor® 680 和 Alexa Fluor® 790分别在荧光显微镜下显示什么颜色?

Asked by: st123456
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

你们提到这个抗体可以应用在FCM上,请问是怎么样使用的?

Asked by: 上科大崇申
感谢您的提问。这边是FCM的protocol, 您可以参考下: https://www.scbt.com/scbt/resources/protocols/flow-cytometry 更多问题,欢迎通过:电话(+86) 021-60936350、邮箱[email protected]、或选择网页上Live Chat中的“中文支持”,联系我们的技术支持部门。 Thank you for your inquiry. Here is the protocol of FCM, https://www.scbt.com/scbt/resources/protocols/flow-cytometry For more questions, please contact our Asian Technical Service team at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Technical Support 15
Date published: 2017-12-15

Can you recommend a positive control to use with Occludin (F-7): sc-271842 monoclonal antibody for Western blot?

Asked by: jenni
We recommend COLO 320DM: sc-2226 whole cell lysate to use as a positive control as we have Western blot data showing specific detection of Occludin in this cell line.
Answered by: Technical Support 15
Date published: 2017-01-12

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Occludin (F-7): sc-271842?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 15
Date published: 2017-01-12
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Rated 5 out of 5 by from Great western dataVery nice western blot data; strong signal with low background
Date published: 2017-01-30
Rated 5 out of 5 by from Strong Occludin antibodyGreat Occludin antibody. Cross-reactive with multiple species--mouse, rat, human, equine, porcine. Works well with endogenous and transfected samples.
Date published: 2017-01-25
Rated 5 out of 5 by from Robust signal in WB in human transfectedRobust signal in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-04-21
Rated 5 out of 5 by from Strong band in WB in COLO 320DM whole cell lysateStrong band in WB in COLO 320DM whole cell lysate. -SCBT QC
Date published: 2015-04-08
Rated 5 out of 5 by from Published IHCP data with mouse colonPublished IHCP data with mouse colon - SCBT Publication Review
Date published: 2014-03-27
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Occludin 抗体 (F-7) is rated 5.0 out of 5 by 5.
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