Date published: 2025-9-8
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Occludin Antibody (E-5) - Immunohistochemistry - Image 158088
Occludin Antibody (E-5): sc-133256. 福尔马林固定, 石蜡包埋的人结肠组织的免疫过氧化物酶染色显示腺细胞的胞浆和膜染色.
Occludin Antibody (E-5): sc-133256. 福尔马林固定, 石蜡包埋的人结肠组织的免疫过氧化物酶染色显示腺细胞的胞浆和膜染色.

Occludin 抗体 (E-5): sc-133256

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说明书
  • Occludin 抗体 E-5 是小鼠单克隆 IgG2b κ，Occludin抗体, 在161篇文献中引用，规格为200 µg/ml
  • 针对氨基酸132-411的图谱，在Occludin的内部区域，human起源
  • Occludin 抗体 (E-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Occludin
  • 抗Occludin抗体（E-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Occludin (E-5): sc-133256的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 Occludin 抗体 (E-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Occludin 抗体 (E-5) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
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说明书与实验方案
研究信息

関連項目

Occludin 抗体（E-5）是一种 IgG2b κ 小鼠单克隆 Occludin 抗体（也称为 OCLN 抗体），可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学（IHC-P）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的 Occludin 蛋白。Occludin 抗体（E-5）既可以是非偶联的抗 Occludin 抗体形式，也可以是多种偶联形式的抗 Occludin 抗体，包括琼脂糖偶联、辣根过氧化物酶（HRP）偶联、藻红蛋白（PE）偶联、异硫氰酸荧光素（FITC）偶联和多种 Alexa Fluor® 偶联物。Occludin 是一种完整的膜蛋白，与上皮和内皮细胞的紧密连接密切相关。Occludin 是上皮和内皮紧密连接（TJ）结构中的四次跨膜蛋白，可包含两个细胞外环。该蛋白质以多种磷酸化形式存在。磷酸化参与调节 Occludin 的定位和功能。Occludin 的表达受多不饱和脂肪酸的上调，增加跨内皮细胞电阻，降低细胞对大分子的通透性。Occludin 的水平因组织而异；在脑组织中，Occludin 在细胞-细胞接触位点高度表达。非神经组织表达较低且分布不连续。上皮 Occludin 的上调可能在增强细胞旁通透性方面发挥作用，并可能与紧密连接的损伤有关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Occludin 抗体 (E-5) 参考文献:

  1. 闭塞素降解使脑部微血管内皮细胞更容易受到体外再灌注损伤。  |  Zhang, Y., et al. 2021. J Neurochem. 156: 352-366. PMID: 32531803
  2. Occludin通过HIF-1α信号通路促进白癜风中CD8+ T细胞和黑色素细胞的粘附  |  Zou, P., et al. 2022. Oxid Med Cell Longev. 2022: 6732972. PMID: 35222802
  3. 成功再灌注后血清中较高的闭孔蛋白与早期神经系统恶化有关。  |  Li, W., et al. 2022. CNS Neurosci Ther. 28: 999-1007. PMID: 35338575
  4. 香烟烟雾诱导的Cathepsin S对Occludin的裂解增加肺上皮细胞的通透性。  |  Bigot, P., et al. 2022. Antioxidants (Basel). 12: PMID: 36670867
  5. 紧密连接蛋白occludin在小鼠缺血性中风后调节血脑屏障的完整性和神经功能。  |  Sugiyama, S., et al. 2023. Sci Rep. 13: 2892. PMID: 36806348
  6. 表皮生长因子受体、Claudin-1和Occludin在丙型肝炎病毒多步骤进入极化肝肿瘤球中的作用。  |  So, CW., et al. 2023. PLoS Pathog. 19: e1011887. PMID: 38157366
  7. Occludin：一种定位于紧密连接处的新型完整膜蛋白。  |  Furuse, M., et al. 1993. J Cell Biol. 123: 1777-88. PMID: 8276896
  8. 紧密连接的分子剖析。  |  Tsukita, S., et al. 1996. Cell Struct Funct. 21: 381-5. PMID: 9118244
  9. occludin 的磷酸化可能参与了紧密连接的形成。  |  Sakakibara, A., et al. 1997. J Cell Biol. 137: 1393-401. PMID: 9182670
  10. Occludin 可能是内皮细胞紧密连接通透性的决定因素。  |  Hirase, T., et al. 1997. J Cell Sci. 110 (Pt 14): 1603-13. PMID: 9247194
  11. occludin 的磷酸化与 occludin 在紧密连接处的定域和功能相关。  |  Wong, V. 1997. Am J Physiol. 273: C1859-67. PMID: 9435490
  12. 多不饱和脂肪酸对紧密连接渗透性和闭塞素表达的调节。  |  Jiang, WG., et al. 1998. Biochem Biophys Res Commun. 244: 414-20. PMID: 9514943

订购信息

产品名称产品编号规格价格数量收藏夹

Occludin 抗体 (E-5)

sc-133256
200 µg/ml
RMB2377.00

Occludin (E-5): m-IgGκ BP-HRP 套装

sc-521292
200 µg Ab, 40 µg BP
RMB2662.00

Occludin (E-5): m-IgG2b BP-HRP 套装

sc-548883
200 µg Ab; 10 µg BP
RMB2662.00

Occludin 抗体 (E-5) AC

sc-133256 AC
500 µg/ml, 25% agarose
RMB3129.00

Occludin 抗体 (E-5) HRP

sc-133256 HRP
200 µg/ml
RMB2377.00

Occludin 抗体 (E-5) FITC

sc-133256 FITC
200 µg/ml
RMB2482.00

Occludin 抗体 (E-5) PE

sc-133256 PE
200 µg/ml
RMB2580.00

Occludin 抗体 (E-5) Alexa Fluor® 488

sc-133256 AF488
200 µg/ml
RMB2685.00

Occludin 抗体 (E-5) Alexa Fluor® 546

sc-133256 AF546
200 µg/ml
RMB2685.00

Occludin 抗体 (E-5) Alexa Fluor® 594

sc-133256 AF594
200 µg/ml
RMB2685.00

Occludin 抗体 (E-5) Alexa Fluor® 647

sc-133256 AF647
200 µg/ml
RMB2685.00

Occludin 抗体 (E-5) Alexa Fluor® 680

sc-133256 AF680
200 µg/ml
RMB2685.00

Occludin 抗体 (E-5) Alexa Fluor® 790

sc-133256 AF790
200 µg/ml
RMB2685.00

Que tipo de anticuerpo secundario es la alternativa para el uso de este anticuerpo primario. En el caso de IHC(P)

Asked by: Revrse
Que tipo de anticuerpo secundario es la alternativa para el uso de este anticuerpo primario. En el caso de IHC(P). https://www.scbt.com/p/m-igg2a-binding-protein-biotin https://www.scbt.com/p/m-igg2a-binding-protein-hrp
Answered by: BlakeJ
Date published: 2025-02-06

I used this antibody for IF,but it have some background,and I could not find the structure of occludin,could you give me some advice .

Asked by: Ning
Thank you for your question. Please contact our Technical Service Department, we are available at [email protected], and happy to help you trouble-shooting.
Answered by: Technical Service SH
Date published: 2021-12-21

Am I amble to sample the antibody before I order it?

Asked by: jrauchut
Thank you for your question. Yes, for a limited time we are offering free 10 µg samples of this mouse monoclonal, Occludin Antibody (E-5): sc-133256. Please contact our Technical Service Department to request a free sample. Please call us toll-free at (800) 457-3801 option 2, e-mail <[email protected]> or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-05-04

Can Occludin (E-5): sc-133256 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, Occludin (E-5): sc-133256 monoclonal antibody has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 10
Date published: 2017-01-12

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Occludin (E-5): sc-133256?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 10
Date published: 2017-01-12
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Rated 5 out of 5 by from Excellent antibodyI used anti-occludin (1:200) and worked perfectly in my breast cancer cell lysates (WB)
Date published: 2023-11-21
Rated 1 out of 5 by from Negative result in pig gut sampleAt necropsy, intestinal tract of pig were dissected. Sample was washed in NaCl 0.9% and post-fixed by immersion for 7 h in 10% buffered formalin (pH 7.4), dehydrated through a graded series of ethanol and finally embedded in Paraplast X-TRA. Four-µm thick sections were cut in the transversal plane and collected on silane-coated slides. After deparaffinization and rehydratation, the Avidin/Biotin blocking was performed and slides were then incubated overnight at room temperature in a dark moist chamber with anti-Occludin (E5) (sc-133256) diluted 1:100 in phosphate buffer saline (PBS). After washing in PBS, sections were incubated for 1.5 hour with Goat Anti-Mouse IgG Antibody (H+L), Biotinylated (6 μg/ml, Vector Laboratories Inc., Burlingame, CA, USA) in a dark moist chamber. The sections were washed twice in PBS and treated with Fluorescein–Avidin D (Vector Laboratories Inc., Burlingame, CA, USA). Finally, the slide with tissue sections was embedded in Vectashield Mounting Medium with DAPI (H-1200, Vector Laboratories Inc., Burlingame, CA, USA) and observed using a Confocal Laser Scanning Microscope (FluoView FV300, Olympus, Tokyo, Japan). Our results did not demonstrate any Occludine-immunopositivity in the epithelial cells of the pig gut mucosa, as expected.
Date published: 2022-12-15
Rated 1 out of 5 by from Negative result in pig gut sampleAt necropsy, intestinal tract of pig were dissected. Sample was washed in NaCl 0.9% and post-fixed by immersion for 7 h in 10% buffered formalin (pH 7.4), dehydrated through a graded series of ethanol and finally embedded in Paraplast X-TRA. Four-µm thick sections were cut in the transversal plane and collected on silane-coated slides. After deparaffinization and rehydratation, the Avidin/Biotin blocking was performed and slides were then incubated overnight at room temperature in a dark moist chamber with anti-Occludin (E5) (sc-133256) diluted 1:100 in phosphate buffer saline (PBS). After washing in PBS, sections were incubated for 1.5 hour with Goat Anti-Mouse IgG Antibody (H+L), Biotinylated (6 μg/ml, Vector Laboratories Inc., Burlingame, CA, USA) in a dark moist chamber. The sections were washed twice in PBS and treated with Fluorescein–Avidin D (Vector Laboratories Inc., Burlingame, CA, USA). Finally, the slide with tissue sections was embedded in Vectashield Mounting Medium with DAPI (H-1200, Vector Laboratories Inc., Burlingame, CA, USA) and observed using a Confocal Laser Scanning Microscope (FluoView FV300, Olympus, Tokyo, Japan). Our results did not demonstrate any Occludine-immunopositivity in the epithelial cells of the pig gut mucosa, as expected.
Date published: 2022-12-14
Rated 1 out of 5 by from Negative result in chickens intestinal sampleI tested this anti-occludin antibody on chicken ileum sample by immunohistochemistry. Antigen retrieval was performed using EDTA buffer (pH 9). Antibody was diluted 1:125 in 1,5% horse serum PBS and incubated 2h at RT. Antibody signal was detected using the avidin-biotin-proxidase method. However, it didn't work and no signal was shown.
Date published: 2022-03-17
Rated 4 out of 5 by from Good antibodyThe antibody works well for IHC staining performed on murine paraffin-embedded tissues. Specific and clear signal, no background. Highly recommended.
Date published: 2021-07-14
Rated 5 out of 5 by from Excelente resultadoCompre este producto hace días y es increíble el resultado
Date published: 2021-01-26
Rated 5 out of 5 by from 美しいバンドが検出されます。WBで使用しました。シャープなバンドが検出されました。非特異的なバンドは一切ありません。バンドの大きさもデータシートと同じ位置です。
Date published: 2020-12-01
Rated 1 out of 5 by from It doesn't work in mouse colon (Western Blot)Dilution 1:500 for Western Blot. Used for detection in mouse colonic tissue extract
Date published: 2020-10-07
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Occludin 抗体 (E-5) is rated 4.2 out of 5 by 37.
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