Date published: 2026-10-20

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Occludin Antibody (F-11) - Western Blotting - Image 377473
Occludin 抗体 (F-11): sc-133255. Caco-2 (A), COLO 205 (B) 和 HUV-EC-C (C) 全细胞裂解液中 Occludin 表达的 Western 印迹分析.
Occludin 抗体 (F-11): sc-133255. Caco-2 (A), COLO 205 (B) 和 HUV-EC-C (C) 全细胞裂解液中 Occludin 表达的 Western 印迹分析.

Occludin 抗体 (F-11): sc-133255

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寫評論提問
说明书
  • Occludin抗体(F-11)是小鼠单克隆IgG1 κ, 在25篇文献中引用，规格为200 µg/ml
  • 针对氨基酸132-411的图谱，在Occludin的内部区域，human起源
  • Occludin 抗体 (F-11) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 Occludin
  • 抗Occludin抗体（F-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Occludin Antibody (F-11) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Occludin Antibody (F-11) 打包提供（请参阅下面的订购信息）。
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関連項目

Occludin 抗体 (F-11) 是一种 IgG1 κ 小鼠单克隆 Occludin 抗体，可通过 WB、IP、IF、IHC(P) 和 ELISA 检测人源性的 Occludin。Occludin 抗体 (F-11) 既可以是非偶联的抗 Occludin 抗体形式，也可以是多种偶联形式的抗 Occludin 抗体，包括琼脂糖、HRP、PE、FITC 和多种 Alexa Fluor® 偶联物。Occludin 是一种与上皮和内皮细胞的紧密连接密切相关的完整膜蛋白。Occludin 是上皮和内皮紧密连接（TJ）结构中的一种四次跨膜完整膜蛋白，可以包含两个细胞外环。该蛋白质以多种磷酸化形式存在。磷酸化参与调节 occludin 的定位和功能。Occludin 的表达受多不饱和脂肪酸的上调，增加跨内皮细胞阻力，降低细胞对大分子的通透性。Occludin 的水平因组织而异；在脑组织中，Occludin 在细胞-细胞接触位点高度表达。非神经组织表达较低且分布不连续。上皮 Occludin 的上调可能在增强细胞旁通透性方面发挥作用，并可能与紧密连接的损伤有关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Occludin 抗体 (F-11) 参考文献:

  1. 炎症诱导的Occludin下调通过抑制Caspase-3表达限制上皮细胞凋亡。  |  Kuo, WT., et al. 2019. Gastroenterology. 157: 1323-1337. PMID: 31401143
  2. IL1B通过上调MIR200C-3p来增加肠道紧密连接的通透性，后者可降解Occludin mRNA。  |  Rawat, M., et al. 2020. Gastroenterology. 159: 1375-1389. PMID: 32569770
  3. 血脑屏障的闭孔调节和缺血性中风中的潜在治疗靶点。  |  Yuan, S., et al. 2020. Brain Circ. 6: 152-162. PMID: 33210038
  4. SNHG12与闭塞素之间的氧适应性相互作用可维持血脑屏障的完整性。  |  Li, Y., et al. 2022. Cell Rep. 39: 110656. PMID: 35417709
  5. tPA 溶栓诱导的缺血性中风出血转化是由 PKCβ 磷酸化闭塞素介导的。  |  Goncalves, A., et al. 2022. Blood. 140: 388-400. PMID: 35576527
  6. 紧密连接蛋白occludin和ZO-1作为上皮细胞增殖和存活的调节因子。  |  Kuo, WT., et al. 2022. Ann N Y Acad Sci. 1514: 21-33. PMID: 35580994
  7. 低温会降低支气管上皮细胞中闭塞素的表达：对寒冷诱发哮喘的影响  |  Zhou, T., et al. 2023. Mol Immunol. 157: 176-185. PMID: 37044043
  8. PHD3通过闭孔蛋白-p38途径抑制结肠癌细胞转移。  |  Li, Y., et al. 2023. Acta Biochim Biophys Sin (Shanghai). 55: 1749-1757. PMID: 37814811
  9. 肺炎链球菌细胞外囊泡通过自噬降解OCLN（封闭蛋白）来加剧肺泡上皮屏障的破坏。  |  Cui, L., et al. 2024. Autophagy. 20: 1577-1596. PMID: 38497494
  10. 闭孔蛋白在中风发病机理中的作用的文献计量学分析。  |  Urasheva, ZU., et al. 2024. Oxid Med Cell Longev. 2024: 2121733. PMID: 39119484

订购信息

产品名称产品编号规格价格数量收藏夹

Occludin 抗体 (F-11)

sc-133255
200 µg/ml
CNY2422.00

Occludin (F-11): m-IgG Fc BP-HRP 套装

sc-528784
200 µg Ab; 10 µg BP
CNY2715.00

Occludin (F-11): m-IgGκ BP-HRP 套装

sc-521291
200 µg Ab, 40 µg BP
CNY2715.00

Occludin (F-11): m-IgG1 BP-HRP 套装

sc-543128
200 µg Ab; 20 µg BP
CNY2715.00

Occludin 抗体 (F-11) AC

sc-133255 AC
500 µg/ml, 25% agarose
CNY3189.00

Occludin 抗体 (F-11) HRP

sc-133255 HRP
200 µg/ml
CNY2422.00

Occludin 抗体 (F-11) FITC

sc-133255 FITC
200 µg/ml
CNY2527.00

Occludin 抗体 (F-11) PE

sc-133255 PE
200 µg/ml
CNY2625.00

Occludin 抗体 (F-11) Alexa Fluor® 488

sc-133255 AF488
200 µg/ml
CNY2738.00

Occludin 抗体 (F-11) Alexa Fluor® 546

sc-133255 AF546
200 µg/ml
CNY2738.00

Occludin 抗体 (F-11) Alexa Fluor® 594

sc-133255 AF594
200 µg/ml
CNY2738.00

Occludin 抗体 (F-11) Alexa Fluor® 647

sc-133255 AF647
200 µg/ml
CNY2738.00

Occludin 抗体 (F-11) Alexa Fluor® 680

sc-133255 AF680
200 µg/ml
CNY2738.00

Occludin 抗体 (F-11) Alexa Fluor® 790

sc-133255 AF790
200 µg/ml
CNY2738.00

Can Occludin (F-11): sc-133255 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, Occludin (F-11): sc-133255 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining.
Answered by: Technical Support 7
Date published: 2017-01-17
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Rated 1 out of 5 by sajjan from Worst antibdy everWe bought this antibdoy thinking that it would work looking at the examples provided on the web page. It never worked in our hands even when used at 1:200 for Western blot analysis. We are using polarized airway epithelial cell lysates in which we usually saw abundat occludin when we were using antibdoy from Cell Signaling, which is now discontinued. As I had experienced before Santa Cruz antibodies are always hit or miss. So you have excess funds go for it
Date published: 2019-07-03
Rated 3 out of 5 by BailuXie from Not as good as other santa cruz antibodiesMay not be good to detect endogenous Ocln in pancreatic cancer cell lines. I tried immunofluorescence, immunoprecipitation and immunoblotting which led to not satisfactory results.
Date published: 2018-10-17
Rated 5 out of 5 by siegerist from Worked in IF-PThe monoclonal Ab worked in immunofluorescent staining of FFPE human kidney sections in 1:100 dilution
Date published: 2017-11-06
Rated 5 out of 5 by dongyj from Strong WBWorks well in Western Blot at 1:1000 with minimal background, published data
Date published: 2017-05-16
Rated 5 out of 5 by Dr Ninau Qelp from Beautiful IHC stainingOccludin (F-11) gives beautiful staining in human, final section of the large intestine tissue, for IHC(P).
Date published: 2017-01-13
Rated 5 out of 5 by Dr__Lee from signal is goodsignal is good
Date published: 2015-12-11
Rated 5 out of 5 by Dane from Highly recommend for IHCHighly recommend for IHC. Wonderful stains on our human tissues samples.
Date published: 2015-10-21
Rated 5 out of 5 by TO from Excellent immunoperoxidase cytoplasmic andExcellent immunoperoxidase cytoplasmic and membrane staining in formalin fixed, paraffin-embedded human rectum tissue. -SCBT QC
Date published: 2015-04-23
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Occludin 抗体 (F-11) is rated 4.3 out of 5 by 9.
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