Date published: 2025-9-8
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Notch1 Antibody (C-10) - Western Blotting - Image 349976
Notch 1 抗体 (C-10): sc-373891. MOLT-4 (A), TE671 (B) 和 MM142 (C) 全细胞裂解液中 Notch 1 表达的 Western 印迹分析.
Notch 1 抗体 (C-10): sc-373891. MOLT-4 (A), TE671 (B) 和 MM142 (C) 全细胞裂解液中 Notch 1 表达的 Western 印迹分析.

Notch1 抗体 (C-10): sc-373891

4.7(7)
寫評論提問

说明书
  • Notch1抗体(C-10)是小鼠单克隆IgG2b κ, 在26篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Notch 1的C-terminus附近的氨基酸2433-2467之间
  • 抗-Notch1 抗体 (C-10) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Notch 1 precursor, mature Notch 1, Notch 1 NEXT and Notch 1 NICD; 也和以下物种反应，包括： equine
  • 抗Notch1抗体（C-10）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Notch1 (C-10): sc-373891的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是Notch1 Antibody (C-10) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Notch1 Antibody (C-10) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Notch抗体，包括Notch 1, Notch 2, Notch 3和Notch 4

Notch 1抗体（C-10）是一种IgG2b κ小鼠单克隆Notch 1抗体，可通过WB、IP、IF和ELISA检测小鼠、大鼠和人类来源的Notch 1前体、成熟Notch 1、Notch 1 NEXT和Notch 1 NICD。Notch 1抗体（C-10）可以作为非偶联抗Notch 1抗体形式，以及多种偶联形式的抗Notch 1抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。LIN-12/Notch跨膜受体被认为在发育过程中通过调节细胞命运决定起核心作用。哺乳动物中已鉴定出四种Notch同源物（Notch 1、Notch 2、Notch 3和Notch 4）。Notch基因在多种胚胎和成人组织中表达，表明这些基因参与多种信号通路。已发现Notch蛋白在人类肿瘤中过表达或重排。Notch配体包括Jagged1、Jagged2和Delta。Jagged1可以通过抑制肌肉调节和结构基因的表达来激活Notch并阻止成肌细胞分化。Jagged2可能参与依赖于上皮间质相互作用的组织发育。除了在肾上腺和胎盘中的正常表达外，Delta表达也在神经内分泌肿瘤中发现。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Notch1 抗体 (C-10)

sc-373891
200 µg/ml
RMB2377.00

Notch1 (C-10): m-IgG Fc BP-HRP 套装

sc-529598
200 µg Ab; 10 µg BP
RMB2662.00

Notch1 (C-10): m-IgGκ BP-HRP 套装

sc-522544
200 µg Ab, 40 µg BP
RMB2662.00

Notch1 (C-10): m-IgG2b BP-HRP 套装

sc-550130
200 µg Ab; 10 µg BP
RMB2662.00

Notch1 抗体 (C-10) AC

sc-373891 AC
500 µg/ml, 25% agarose
RMB3129.00

Notch1 抗体 (C-10) HRP

sc-373891 HRP
200 µg/ml
RMB2377.00

Notch1 抗体 (C-10) FITC

sc-373891 FITC
200 µg/ml
RMB2482.00

Notch1 抗体 (C-10) PE

sc-373891 PE
200 µg/ml
RMB2580.00

Notch1 抗体 (C-10) Alexa Fluor® 488

sc-373891 AF488
200 µg/ml
RMB2685.00

Notch1 抗体 (C-10) Alexa Fluor® 546

sc-373891 AF546
200 µg/ml
RMB2685.00

Notch1 抗体 (C-10) Alexa Fluor® 594

sc-373891 AF594
200 µg/ml
RMB2685.00

Notch1 抗体 (C-10) Alexa Fluor® 647

sc-373891 AF647
200 µg/ml
RMB2685.00

Notch1 抗体 (C-10) Alexa Fluor® 680

sc-373891 AF680
200 µg/ml
RMB2685.00

Notch1 抗体 (C-10) Alexa Fluor® 790

sc-373891 AF790
200 µg/ml
RMB2685.00

Notch1 (C-10) 中和勝肽

sc-373891 P
100 µg/0.5 ml
RMB511.00

can be used for chip ? thanks

Asked by: Fahmi
Thank you for your question. We do not yet have any data or product citations using this antibody for a ChIP application.
Answered by: Technical Support
Date published: 2019-08-22

What application is the blocking peptide sc-373891 P appropriate for?

Asked by: Dr Ninau Qelp
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from good antibodyClean and single dark band detected at MW of 110kDa
Date published: 2020-02-07
Rated 5 out of 5 by from Great antibody, clear bands in western blot.I received a sample of this antibody (10ug/50ul). I tested it on protein extracts (30ug) of a cell line of trophoblasts induced to differentiate to an endothelial-like phenotype. First antibody dilution: 1: 1000 (0.2 ug/ml) Dilution second anticuepo (anti mouse - HRP): 1: 5000 Detection: ECL Exposure time: 45 sec. Approx.
Date published: 2018-05-14
Rated 4 out of 5 by from 功能还可以我一个月前申请使用本产品，一抗配比浓度为1：500，显影结果有杂带，背景稍高，目的条带显示不清，抗体使用时间仅为一个月。
Date published: 2018-05-09
Rated 5 out of 5 by from Excellent in IF!Bright signal seen in IF with nuclear localization.
Date published: 2017-01-19
Rated 5 out of 5 by from Robust band observed in western blot analysisRobust band observed in western blot analysis of truncated mouse Notch 1 transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-03-12
Rated 5 out of 5 by from Produced superb immunofluorescence nuclearProduced superb immunofluorescence nuclear staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-01-31
Rated 5 out of 5 by from Clean immunohistochemical stain using 1:50Clean immunohistochemical stain using 1:50 dilution on human mammary gland/tumor sections; also used for WB. -SCBT Publication Review
Date published: 2015-01-16
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Notch1 抗体 (C-10) is rated 4.7 out of 5 by 7.
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