Date published: 2025-9-7
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Notch1 Antibody (mN1A) - Western Blotting - Image 149412
Notch 1 抗体 (mN1A): sc-32745. MM-142 (A), F9 (B) 和 LADMAC (C) 全细胞裂解液中 Notch 1 表达的 Western 印迹分析.
Notch 1 抗体 (mN1A): sc-32745. MM-142 (A), F9 (B) 和 LADMAC (C) 全细胞裂解液中 Notch 1 表达的 Western 印迹分析.

Notch1 抗体 (mN1A): sc-32745

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说明书
  • Notch1抗体(mN1A)是小鼠单克隆IgG1 κ, 在27篇文献中引用，规格为200 µg/ml
  • 针对Notch 1的cdc10-NCR区域（mouse起源）
  • 抗-Notch1 抗体 (mN1A) 推荐用于 WB 和 IP，检测mouse, rat 和human 来源的 Notch 1
  • 抗Notch1抗体（mN1A）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Notch1 (mN1A): sc-32745的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是Notch1 Antibody (mN1A) for WB applications. 的首选辅助检测试剂，这些试剂现与Notch1 Antibody (mN1A) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Notch抗体，包括Notch 1, Notch 2, Notch 3和Notch 4

Notch1抗体（mN1A）是一种小鼠单克隆IgG1 kappa轻链抗体，用于通过蛋白质印迹（WB）和免疫沉淀（IP）在小鼠、大鼠和人类样本中检测Notch1（也称为神经源性位点缺口同源蛋白1）。Notch1是一种单跨膜受体，在胚胎和成体发育过程中对细胞命运的确定起着关键作用。当与配体（如Jagged1、Jagged2和Delta样蛋白）结合后，Notch1会经历蛋白水解，释放出其细胞内结构域，后者会转移到细胞核中以调节基因表达。这种信号传导通路对分化、增殖和凋亡等过程至关重要，影响着各种组织和器官的发育。Notch1的失调与多种癌症有关，包括T细胞急性淋巴细胞白血病，这凸显了其在维持细胞稳态方面的重要性。抗Notch1抗体（mN1A）有非结合型和多种 结合形式，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）以及各种Alexa Fluor®结合物，以满足不同的实验需求。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Notch1 抗体 (mN1A) 参考文献:

  1. Notch2：哺乳动物的第二个 Notch 基因。  |  Weinmaster, G., et al. 1992. Development. 116: 931-41. PMID: 1295745
  2. 小檗属植物抑制糖尿病视网膜病变的血管生成和细胞凋亡，其机制是通过抑制HIF-1α/VEGF/DLL-4/Notch-1通路。  |  O'Brien, R. and Marignol, L. 2017. Cancer Treat Rev. 56: 36-46. PMID: 28457880
  3. 血清前白蛋白水平与社区获得性细菌性脑膜炎成人预后的关系：一项回顾性队列研究。  |  Ai, X., et al. 2022. J Ethnopharmacol. 296: 115453. PMID: 35697191
  4. 干细胞、Notch-1信号和氧化应激：呼吸道癌症发展和进展中的地狱三重奏。天然化合物是否发挥作用？  |  Gao, Q., et al. 2023. Aging (Albany NY). 15: 14323-14332. PMID: 38095642
  5. 天麻素通过肾素-血管紧张素系统调节活化小胶质细胞中的Notch-1信号通路。  |  Zhang, M., et al. 2023. Aging (Albany NY). 15: 15196-15212. PMID: 38149979
  6. 胰岛素抵抗的生物化学机制。  |  Chiappara, G., et al. 2024. Carcinogenesis. 45: 621-629. PMID: 39046986
  7. Jagged：一种能激活 Notch1 的哺乳动物配体。  |  Lindsell, CE., et al. 1995. Cell. 80: 909-17. PMID: 7697721
  8. Notch1对小鼠植入后的发育至关重要。  |  Swiatek, PJ., et al. 1994. Genes Dev. 8: 707-19. PMID: 7926761
  9. dlk，一种在小细胞肺癌和神经内分泌肿瘤细胞系中差异表达的哺乳动物推测同源基因。  |  Laborda, J., et al. 1993. J Biol Chem. 268: 3817-20. PMID: 8095043
  10. 小鼠缺口：在毛囊中的表达与细胞命运的决定有关。  |  Kopan, R. and Weintraub, H. 1993. J Cell Biol. 121: 631-41. PMID: 8486742
  11. Notch4/int-3 是一种乳腺原癌基因，是哺乳动物内皮细胞特异性 Notch 基因。  |  Uyttendaele, H., et al. 1996. Development. 122: 2251-9. PMID: 8681805
  12. MMTVD/myc转基因小鼠胸腺瘤中Notch1的频繁病毒插入突变表明c-myc和Notch1共同参与了肿瘤的发生。  |  Girard, L., et al. 1996. Genes Dev. 10: 1930-44. PMID: 8756350

订购信息

产品名称产品编号规格价格数量收藏夹

Notch1 抗体 (mN1A)

sc-32745
200 µg/ml
RMB2377.00

Notch1 (mN1A): m-IgG Fc BP-HRP 套装

sc-526685
200 µg Ab; 10 µg BP
RMB2662.00

Notch1 (mN1A): m-IgG1 BP-HRP 套装

sc-532058
200 µg Ab; 20 µg BP
RMB2662.00

Notch1 抗体 (mN1A) AC

sc-32745 AC
500 µg/ml, 25% agarose
RMB3129.00

Notch1 抗体 (mN1A) HRP

sc-32745 HRP
200 µg/ml
RMB2377.00

Notch1 抗体 (mN1A) FITC

sc-32745 FITC
200 µg/ml
RMB2482.00

Notch1 抗体 (mN1A) PE

sc-32745 PE
200 µg/ml
RMB2580.00

Notch1 抗体 (mN1A) Alexa Fluor® 488

sc-32745 AF488
200 µg/ml
RMB2685.00

Notch1 抗体 (mN1A) Alexa Fluor® 546

sc-32745 AF546
200 µg/ml
RMB2685.00

Notch1 抗体 (mN1A) Alexa Fluor® 594

sc-32745 AF594
200 µg/ml
RMB2685.00

Notch1 抗体 (mN1A) Alexa Fluor® 647

sc-32745 AF647
200 µg/ml
RMB2685.00

Notch1 抗体 (mN1A) Alexa Fluor® 680

sc-32745 AF680
200 µg/ml
RMB2685.00

Notch1 抗体 (mN1A) Alexa Fluor® 790

sc-32745 AF790
200 µg/ml
RMB2685.00

What dilution and detecting agent to use for this antibody? I am having trouble detecting this. Picture in the data sheet looks good.

Asked by: Silvia01
Thank you for your question. We used the primary antibody, Notch 1 (mN1A): sc-32745, at a dilution of 1:200 for the Western blot data shown on our product data sheet. We used a conventional anti-mouse IgG-HRP conjugated secondary antibody, and our Luminol (sc-2048) for detection. A link to our complete recommended protocol for Western blotting with this antibody is provided below. If you continue to have trouble, please contact our Technical Service Department and we are happy to help you troubleshoot the antibody. Please call us toll-free at (800) 457-3801 option 2, e-mail [email protected], or use our live chat. https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-04-19

Is Notch 1 (mN1A): sc-32745 monoclonal antibody available directly conjugated to HRP or fluorescent dyes?

Asked by: jenniferc
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at [email protected] Alternatively, consider using Notch 1 (A-8): sc-376403, which is available conjugated to agarose, HRP, FITC, PE, and more. (https://www.scbt.com/scbt/product/notch-1-antibody-a-8)
Answered by: Technical Support 5
Date published: 2017-01-22
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Rated 4 out of 5 by from good for WBThis antibody worked good for western blot analysis at 2.0 μg/ml in mouse embryonic telencephalons.
Date published: 2023-02-21
Rated 4 out of 5 by from Great way to compare isoformsBy using the trial antibody size this allowed me to test a variety of different isoforms of this antibody to see which works best in my model of mouse. Great way to make an economic decision before purchasing in bulk!
Date published: 2017-11-29
Rated 4 out of 5 by from great antibodyWorks well but may have some background. 1:200 in milk
Date published: 2017-08-31
Rated 3 out of 5 by from Not for zebrafishReceived a sample for IHC with zebrafish tissue. Not reactive with zebrafish.
Date published: 2017-08-15
Rated 5 out of 5 by from validated KO Notch 1 antibodyThis mouse monoclonal has been validated for specificity for Notch 1 using siRNA gene silencing knockdown experiments performed by SCBT.
Date published: 2016-12-20
Rated 5 out of 5 by from This antibody is stable for dectecting Notch1This antibody is stable for dectecting Notch1 in protein lysates of human/mouse T,B cells and lymphoma cells, and worked very well.
Date published: 2015-11-02
Rated 5 out of 5 by from Worked very well to distinguish between knockoutWorked very well to distinguish between knockout and endogenous.
Date published: 2015-09-28
Rated 5 out of 5 by from We used this in WB and were satisfied withWe used this in WB and were satisfied with our results. Good signal.
Date published: 2015-07-27
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Notch1 抗体 (mN1A) is rated 4.5 out of 5 by 13.
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