Date published: 2025-9-7

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Nek2 抗体 (D-8): sc-55601

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说明书
  • Nek2 抗体 D-8 是小鼠单克隆 IgG2b κ,Nek2抗体, 在33篇文献中引用,规格为200 µg/ml
  • 针对氨基酸211-445,在Nek2的human起源的C-terminus处进行映射
  • Nek2 抗体 (D-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Nek2
  • 抗Nek2抗体(D-8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Nek2 (D-8): sc-55601免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRPm-IgGκ BP-HRP 是 Nek2 抗体 (D-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Nek2 抗体 (D-8) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

Nek2抗体(D-8)是一种小鼠单克隆IgG2b kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的Nek2蛋白 )。抗Nek2抗体(D-8)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。Nek2在细胞周期调节中发挥着关键作用,特别是在G2期到有丝分裂的过渡期,其表达达到峰值。这种调节对于正常的细胞分裂至关重要,因为Nek2活性异常会导致基因组不稳定并促进肿瘤发生。此外,Nek2与在曲霉菌中发现的NIMA蛋白密切相关,后者对于有丝分裂的进入至关重要,这突出了功能进化的保守性。在哺乳动物系统中,Nek2的表达受到严格调控,失调与多种癌症有关,因此Nek2成为重要的研究及治疗干预目标。Nek2 (D-8)抗体可有效检测多种物种和应用,对于研究细胞周期动态和相关病理的研究人员来说,该工具非常宝贵。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Nek2 抗体 (D-8) 参考文献:

  1. 小鼠 Nek3 蛋白激酶的克隆与表征,Nek3 蛋白激酶是推定细胞周期调节因子 NIMA 家族的新成员。  |  Tanaka, K. and Nigg, EA. 1999. J Biol Chem. 274: 13491-7. PMID: 10224116
  2. 与 NIMA 相关的激酶:小鼠 nek3 和 nek4 cDNA 的分离和表征,以及 nek1, nek2 和 nek3 的染色体定域。  |  Chen, A., et al. 1999. Gene. 234: 127-37. PMID: 10393247
  3. 哺乳动物双特异性蛋白激酶 Nek1 与 NIMA 细胞周期调节因子有关,在减数分裂生殖细胞中高度表达。  |  Letwin, K., et al. 1992. EMBO J. 11: 3521-31. PMID: 1382974
  4. 通过过量表达编码潜在蛋白激酶的 G2 特异性基因来诱导和维持有丝分裂。  |  Osmani, SA., et al. 1988. Cell. 53: 237-44. PMID: 3359487
  5. 与黑曲霉有丝分裂调节因子 NIMA 有关的新型人类蛋白激酶 Nek2 的细胞周期依赖性表达。  |  Schultz, SJ., et al. 1994. Cell Growth Differ. 5: 625-35. PMID: 7522034
  6. NIMA 相关激酶 2(Nek2)在减数分裂细胞周期的特定阶段表达,并与减数分裂染色体缔合作用。  |  Rhee, K. and Wolgemuth, DJ. 1997. Development. 124: 2167-77. PMID: 9187143
  7. 哺乳动物 NIMA 相关激酶的特征。  |  Fry, AM. and Nigg, EA. 1997. Methods Enzymol. 283: 270-82. PMID: 9251026

订购信息

产品名称产品编号规格价格数量收藏夹

Nek2 抗体 (D-8)

sc-55601
200 µg/ml
RMB2377.00

Nek2 (D-8): m-IgG Fc BP-HRP 套装

sc-528610
200 µg Ab; 10 µg BP
RMB2662.00

Nek2 (D-8): m-IgGκ BP-HRP 套装

sc-521030
200 µg Ab, 40 µg BP
RMB2662.00

Nek2 (D-8): m-IgG2b BP-HRP 套装

sc-550057
200 µg Ab; 10 µg BP
RMB2662.00

Nek2 抗体 (D-8) AC

sc-55601 AC
500 µg/ml, 25% agarose
RMB3129.00

Nek2 抗体 (D-8) HRP

sc-55601 HRP
200 µg/ml
RMB2377.00

Nek2 抗体 (D-8) FITC

sc-55601 FITC
200 µg/ml
RMB2482.00

Nek2 抗体 (D-8) PE

sc-55601 PE
200 µg/ml
RMB2580.00

Nek2 抗体 (D-8) Alexa Fluor® 488

sc-55601 AF488
200 µg/ml
RMB2685.00

Nek2 抗体 (D-8) Alexa Fluor® 546

sc-55601 AF546
200 µg/ml
RMB2685.00

Nek2 抗体 (D-8) Alexa Fluor® 594

sc-55601 AF594
200 µg/ml
RMB2685.00

Nek2 抗体 (D-8) Alexa Fluor® 647

sc-55601 AF647
200 µg/ml
RMB2685.00

Nek2 抗体 (D-8) Alexa Fluor® 680

sc-55601 AF680
200 µg/ml
RMB2685.00

Nek2 抗体 (D-8) Alexa Fluor® 790

sc-55601 AF790
200 µg/ml
RMB2685.00

Does this antibody work for co-IP?

Asked by: PENDF
Thank you for your question. Yes, this antibody is great for Co-IP. We have several recent publications, here are a few examples: PMID: 30370607, 29863498.
Answered by: Tech Service
Date published: 2019-12-10

I need to use an antibody to detect the protein in samples of equine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 3
Date published: 2017-02-01
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Rated 5 out of 5 by from Great antibody for western blottingHave been using this antibody for years to detect NEK2 in various human cell lines and primary cells with good results. 1:1000 dilution works well for most applications but if protein expression is low, I've used 1:500 and 1:250 with minimal background.
Date published: 2025-01-06
Rated 5 out of 5 by from Works well in both human and mouseBought this product to try and detect NEK2 in mouse bone marrow as the other most cited antibody from BD failed to work. Used the antibody at 1:100 to be safe and got a nice band in both my cell line and mouse bone marrow!
Date published: 2022-05-30
Rated 4 out of 5 by from Good Antibody working well for WBWe used this antibody to evaluate expression levels and silencing efficiency for NEK2 protein in multiple cell lines and obtained very good results.
Date published: 2021-12-22
Rated 5 out of 5 by from Good signal in WBWorked very well for western blotting using lysates from human cell lines.
Date published: 2020-11-16
Rated 1 out of 5 by from Does not work for IHC or IFTried tw different clones of this antibody, and none of them work for IHC or IF. No staining.
Date published: 2019-04-16
Rated 4 out of 5 by from good productWe used 1:1000 dilution to do WB in U2OS whole cell lysate. The signal is very weak, needs long exposure (about 2 hours) to get the signal with some non-specific bands.
Date published: 2018-02-09
Rated 4 out of 5 by from Specific SignalWorked well and specifically on mouse tissues using WB.
Date published: 2018-01-24
Rated 5 out of 5 by from Sehr gute Ergebnisse in der Immunfluoreszenz.Sehr gute Ergebnisse in der Immunfluoreszenz mit humanen und murinen Zelllinien (NIH, hTERT RPE-1). Es wurde die Verdünnung 1:500 benutzt.
Date published: 2018-01-04
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Nek2 抗体 (D-8) is rated 4.6 out of 5 by 23.
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