Date published: 2025-9-7
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mTOR Antibody (30) - Western Blotting - Image 390045
mTOR 抗体 (30) HRP: sc-517464 HRP. 在 HeLa (A), HeLa + Calculin A (B), Jurkat (C), Jurkat + Calculin A (D), CCRF-CEM (E) 和 TK-1 (F) 全细胞裂解液中直接进行 mTOR 表达的 western 印迹分析.
mTOR 抗体 (30) HRP: sc-517464 HRP. 在 HeLa (A), HeLa + Calculin A (B), Jurkat (C), Jurkat + Calculin A (D), CCRF-CEM (E) 和 TK-1 (F) 全细胞裂解液中直接进行 mTOR 表达的 western 印迹分析.

mTOR 抗体 (30): sc-517464

4.3(12)
寫評論提問

说明书
  • mTOR抗体(30)是小鼠单克隆IgG1 λ mTOR抗体, 在121篇文献中引用，规格为200 µg/ml
  • 免疫rat物种的的氨基酸185-290
  • 推荐用于 mouse, rat 和 human 来源的mTOR WB, IP 和 IF检测
  • 还可偶联琼脂糖 (sc-517464 AC), 500 µg/ml, 25% agarose, 用于IP; 偶联HRP (sc-517464 HRP), 200 µg/ml, 用于WB, IHC(P) 和ELISA
  • 关于如何获取mTOR (30): sc-517464的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是mTOR Antibody (30) for WB applications. 的首选辅助检测试剂，这些试剂现与mTOR Antibody (30) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

mTOR抗体（30）是一种小鼠单克隆IgG1 lambda轻链抗体，用于检测雷帕霉素（mTOR）蛋白的机制靶标，识别大鼠来源的氨基酸区域185-290。mTOR，也称为SKS、FRAP 、FRAP1、FRAP2、RAFT1和RAPT1，是一种关键的丝氨酸/苏氨酸激酶，通过整合来自营养物质、生长因子和细胞能量状态的信号，在调节细胞生长、增殖、代谢和自噬方面发挥着关键作用。mTOR主要位于细胞质中，在mTORC1和mTORC2两个不同的复合物中发挥作用，每个复合物协调不同的细胞过程，这些过程对于维持细胞稳态和响应环境信号至关重要。mTOR的结构包括一个保守的激酶结构域和多个调节区域，这是其能够磷酸化下游靶标（如S6激酶和4E-BP1）从而控制蛋白质合成和细胞周期进展的基础。翻译后修饰，包括p70S6激酶在Ser2481处的自磷酸化作用和在Ser2448处的磷酸化作用，对于调节mTOR活性及其与其他蛋白质的相互作用至关重要。mTOR与多种蛋白质相互作用 ，包括Raptor和Rictor（分别是mTORC1和mTORC2的组成部分），以及TSC1/2和AMPK等调节蛋白，这些蛋白可调节mTOR的活性，以应对细胞应激和营养供应。这些相互作用和修饰对于mTOR在细胞信号通路中发挥作用至关重要，从而影响生长和存活，使mTOR成为癌症、神经退行性疾病和代谢性疾病研究的重要目标。抗mTOR抗体（30）已验证可用于小鼠、大鼠和人类样本的蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）实验，确保在各种实验设置中具有广泛的适用性。此外，mTOR (30) 抗体有非结合型和结合型两种形式，包括琼脂糖和辣根过氧化物酶 (HRP)，为各种实验室应用提供了灵活性，并有助于在复杂的细胞环境中检测和研究 mTOR。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

mTOR 参考文献:

  1. 血清前白蛋白可预测血液透析和腹膜透析的存活率：10年的前瞻性观察。  |  Kim, DH., et al. 2002. Cell. 110: 163-75. PMID: 12150925
  2. mTOR途径中的翻译调控。  |  Mossmann, D., et al. 2018. Nat Rev Cancer. 18: 744-757. PMID: 30425336
  3. 以mTOR为靶点进行抗衰老和抗癌治疗。  |  Deleyto-Seldas, N. and Efeyan, A. 2021. Front Cell Dev Biol. 9: 655731. PMID: 34277603
  4. mTOR-自噬轴与代谢控制。  |  Yang, M., et al. 2022. Biomolecules. 12: PMID: 35740927
  5. mTOR在细胞程序性死亡中的作用及其治疗意义。  |  Mao, B., et al. 2022. Molecules. 27: PMID: 36014530
  6. mTOR抑制剂研究概述。  |  Fu, W. and Wu, G. 2023. Molecules. 28: PMID: 37049920
  7. mTOR与raptor相互作用，形成营养敏感复合物，向细胞生长机制发出信号。  |  Xie, Y., et al. 2023. Cytokine Growth Factor Rev. 71-72: 66-81. PMID: 37380596
  8. 什么情况下脂质激酶不是脂质激酶？当它是一种蛋白激酶时。  |  Hunter, T. 1995. Cell. 83: 1-4. PMID: 7553860
  9. PIK 相关激酶：DNA 修复, 重组和细胞周期检查点。  |  Keith, CT. and Schreiber, SL. 1995. Science. 270: 50-1. PMID: 7569949
  10. DNA 依赖性蛋白激酶催化亚基：磷脂酰肌醇 3-激酶和共济失调毛细血管扩张症基因产物的亲缘关系。  |  Hartley, KO., et al. 1995. Cell. 82: 849-56. PMID: 7671312
  11. AT基因的发现引发了生物医学研究的繁荣。  |  Nowak, R. 1995. Science. 268: 1700-1. PMID: 7792589
  12. 单个共济失调毛细血管扩张症基因的产物与 PI-3 激酶相似。  |  Savitsky, K., et al. 1995. Science. 268: 1749-53. PMID: 7792600

订购信息

产品名称产品编号规格价格数量收藏夹

mTOR 抗体 (30)

sc-517464
200 µg/ml
RMB2377.00

mTOR (30): m-IgG Fc BP-HRP 套装

sc-528075
200 µg Ab; 10 µg BP
RMB2662.00

mTOR (30): m-IgG1 BP-HRP 套装

sc-533448
200 µg Ab; 20 µg BP
RMB2662.00

mTOR 抗体 (30) AC

sc-517464 AC
500 µg/ml, 25% agarose
RMB3129.00

mTOR 抗体 (30) HRP

sc-517464 HRP
200 µg/ml
RMB2377.00

Do you have a Magnetic Beads conjugated version of this Ab?

Asked by: Bo888
Thank you for your question. We do not have a magnetic bead conjugated form but sc-517464 AC can be used for IP.
Answered by: BlakeJ
Date published: 2025-04-23

Is mTORC1 around 280kd ？Where is mTORC2？

Asked by: wen1113
感谢提问。 我们可以从对应货号的官网说明书中，找到对应产品的蛋白分子量大小。 Thank you for your question. We can check the protein MW from Datasheets of official website.
Answered by: Ling Chen
Date published: 2022-06-27

where is the location of the mTOR? 289kd?

Asked by: xgasy
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-01-28

Can you make FITC or PE-conjugated anti-mTOR antibodies?

Asked by: lerador
Thank you for your question. This is considered a special order request. Please contact our Special Orders department by email at [email protected] .
Answered by: Blake J
Date published: 2021-07-29

Hi, when this was delivered it was left out on the bench for 24 hours at room temp. Will this affect the function of the antibody?

Asked by: Laken2709
Thank you for your question. We guarantee our antibodies for up to 5 days in the package at room temperature. As long as it was placed in a fridge within that time frame, the antibody should be fine. -Tech Service
Answered by: Tech Service 1
Date published: 2021-02-04

Hi, Is this usable for the total mTOR?

Asked by: Bichan
Thank you for your question. mTOR Antibody (30): sc-517464 recognizes both phosphorylated and non-phosphorylated mTOR of mouse, rat and human origin in WB, IP and IF assays.
Answered by: Tech Support Europe
Date published: 2020-12-31

is it mTOR1 or mTOR2 or pan mTOR?

Asked by: pilar
Thank you for your question. This antibody was made against a mTOR 1 sequence, however, it will detect both mTOR forms.
Answered by: Tech Service
Date published: 2019-08-21
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Rated 5 out of 5 by from The mTOR Ab worked well in WB and IHCThe mTOR Ab worked well in WB and IHC of human and mouse samples
Date published: 2024-05-30
Rated 4 out of 5 by from anti-mTOR works for WB analysisanti-mTOR antibody detects mTOR in human cells. We used this antibody for Western blot analysis.
Date published: 2024-01-15
Rated 5 out of 5 by from total mTOR Western blot applicationStrong band detected in mouse crude synaptosomal fraction at the expected molecular weight. Additional band detected around 100 kDa. Antibody dilution 1:500.
Date published: 2023-04-13
Rated 5 out of 5 by from 分子量准确，使用说明完整。服务很周到，产品质量过关，会推荐购买，到货速度也很快，产品说明书很完善，使用起来完全没有困难，值得推荐。
Date published: 2023-02-09
Rated 4 out of 5 by from Good western blot signalGood western blot signal in mouse tissue. Diluition 1:1000
Date published: 2022-03-24
Rated 3 out of 5 by from Specific to mTOR but many other bandsI bought this antibody a couple of weeks ago and tested it right away on Western blot. Although the antibody is not very specific (several bands), the specificity to mTOR seems good (the band for mTOR disappears with mTOR knock-down).
Date published: 2020-09-07
Rated 5 out of 5 by from Great specificityI brought this antibody a couple of weeks ago. This antibody works fine giving very specific band.
Date published: 2020-04-05
Rated 4 out of 5 by from goodWorked well, band at predicted 250KDa, second band around 120KDa, and a third at 100kDa
Date published: 2020-02-06
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mTOR 抗体 (30) is rated 4.3 out of 5 by 12.
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