Date published: 2025-9-7

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MHC class I 抗体 (ER-HR52): sc-59199

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说明书
  • MHC class I 抗体 ER-HR52 是大鼠单克隆 IgG2a, 在10篇文献中引用,提供 200 µg/ml
  • 在mouse来源的巨噬细胞前体细胞上
  • MHC class I 抗体 (ER-HR52) 推荐用于WB, IP 和 IF,检测mouse 来源的 MHC class I molecules on the surface of cells of the following haplotypes: H2-Db, H2-Dw16, H2d,p,q and, to a lesser extent, H2f,r,s,w17,w23,w27; 不会与 MHC class I molecules of other haplotypes产生交联反应
  • MHC class I抗体(ER-HR52)可与琼脂糖结合用于IP,与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MHC class I (ER-HR52): sc-59199免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成MHC class I 抗体 (ER-HR52)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

MHC I类抗体(ER-HR52)是一种鼠单克隆IgG2a抗体,用于检测细胞表面主要组织相容性复合物I类(MHC I)分子,在小鼠中也称为H-2分子, ,包括H2-Db、H2-Dw16、H2d、p、q,以及少量H2f、r、s、w17、w23、w27。抗MHC I类抗体(ER-HR52)靶向位于质膜上的MHC I类蛋白,后者对于向细胞毒性T淋巴细胞呈递内源性肽至关重要,并在免疫监视和反应中发挥关键作用。MHC I类(ER-HR52)抗体在多种应用中均有效,例如:蛋白质印迹(WB)、免疫沉淀(IP)和免疫荧光(IF),确保在各种研究环境中具有广泛用途。抗原决定簇针对小鼠来源的巨噬细胞前体细胞,提高了针对指定MHC I类分子的特异性和结合亲和力。MHC I类(ER-HR52)单克隆抗体有结合和非结合两种形式,适用于各种检测方法和实验设计。抗MHC I类抗体(ER-HR52)能够特异性识别细胞表面的MHC I类蛋白,对于涉及抗原呈递、免疫细胞相互作用和相关免疫学研究的研究具有不可估量的价值。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

MHC class I 抗体 (ER-HR52)

sc-59199
200 µg/ml
RMB2377.00

MHC class I 抗体 (ER-HR52) AC

sc-59199 AC
500 µg/ml, 25% agarose
RMB3129.00

MHC class I 抗体 (ER-HR52) FITC

sc-59199 FITC
200 µg/ml
RMB2482.00

MHC class I 抗体 (ER-HR52) PE

sc-59199 PE
200 µg/ml
RMB2580.00

MHC class I 抗体 (ER-HR52) Alexa Fluor® 488

sc-59199 AF488
200 µg/ml
RMB2685.00

MHC class I 抗体 (ER-HR52) Alexa Fluor® 546

sc-59199 AF546
200 µg/ml
RMB2685.00

MHC class I 抗体 (ER-HR52) Alexa Fluor® 594

sc-59199 AF594
200 µg/ml
RMB2685.00

MHC class I 抗体 (ER-HR52) Alexa Fluor® 647

sc-59199 AF647
200 µg/ml
RMB2685.00

MHC class I 抗体 (ER-HR52) Alexa Fluor® 680

sc-59199 AF680
200 µg/ml
RMB2685.00

MHC class I 抗体 (ER-HR52) Alexa Fluor® 790

sc-59199 AF790
200 µg/ml
RMB2685.00

When the antibody MHC class I (sc-59199) is used for immunofluorescence, can i use an anti-mouse fluorescent secondary antobody?

Asked by: Maria9
Thank you for your question. MHC class I (ER-HR52) antibody is a rat IgG2a antibody so a more sutiable secondary would be a anti rat secondary antibody .Please contact our Asia Technical Support team for additional information. You may reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com.
Answered by: Blake J
Date published: 2021-07-08

Can MHC class I (ER-HR52): sc-59199 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, MHC class I (ER-HR52): sc-59199 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-03-01
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Rated 4 out of 5 by from WB功能得到了应用大约2个月前购买了此产品,WB功能得到了应用,效果和实例图片差不多,这个靶标的抗体产品比较难买,希望santa有更多好产品。
Date published: 2022-02-07
Rated 5 out of 5 by from Worked great for murine dcsWorked great for murine dcs by flow cytometric detection!
Date published: 2019-12-04
Rated 5 out of 5 by from Produced publishable WB data in mouse and human.This MHC class I monoclonal antibody has several citations in the primary literature for the use in WB, IF and IHC on mouse and human cells. H292,H3255,H441,HFL-1, MEF, Placental and intestinal tissue samples and mouse T-cells.
Date published: 2017-01-24
Rated 5 out of 5 by from published in 2011: IF(pfa) of mouse tpublished in 2011: IF(pfa) of mouse t-cells
Date published: 2015-11-06
Rated 5 out of 5 by from Robust signal in WB in CTLLRobust signal in WB in CTLL-2 whole cell lysate. -SCBT QC
Date published: 2014-06-27
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MHC class I 抗体 (ER-HR52) is rated 4.8 out of 5 by 5.
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