Date published: 2025-9-7
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
MHC class I Antibody (W6/32) - Immunofluorescence - Image 12126
MHC I 类抗体 (W6/32): sc-32235. 甲醇固定的 Raji 细胞免疫荧光染色, 显示膜定位.
MHC I 类抗体 (W6/32): sc-32235. 甲醇固定的 Raji 细胞免疫荧光染色, 显示膜定位.

MHC class I 抗体 (W6/32): sc-32235

3.9(17)
寫評論提問

说明书
  • MHC class I 抗体 W6/32 是小鼠单克隆 IgG2a κ，MHC class I抗体, 在39篇文献中引用，规格为200 µg/ml
  • MHC class I 抗体 (W6/32) 推荐用于WB, IP, IF 和 FCM，检测human 来源的 HLA-A, HLA-B and HLA-C
  • 识别human来源的HLA-A、B和C抗原共有的W6/32抗原决定簇
  • 抗MHC class I抗体（W6/32）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素偶联抗体，可用于WB、IHC(P)和ELISA实验
  • 关于如何获取MHC class I (W6/32): sc-32235的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是MHC class I Antibody (W6/32) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与MHC class I Antibody (W6/32) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Major Histocompatibility Complex抗体，包括MHC class I, MHC class I α, MHC class Ib, MHC class II, MHC class II β, MHC class II DR和MHC class II I E

MHC I类抗体（W6/32）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和流式细胞术（FCM）特异性检测人类HLA-A、HLA-B和HLA-C分子。MHC I类单克隆抗体（W6/32）可识别人类MHC I类分子重链上的单态表位，从而广泛检测这些重要的细胞表面蛋白。MHC I类分子，也称为人类白细胞抗原（HLA）I类，包括HLA-A、HLA-B和HLA-C，对于免疫系统向细胞毒性T淋巴细胞（CD8+ T细胞）呈递内源性肽抗原的能力至关重要。MHC I类分子通过呈现通常为8-10个氨基酸的肽，使免疫系统能够监控细胞健康并识别受感染或恶性的细胞。MHC I类分子的功能对于免疫监控和启动细胞毒性免疫反应至关重要，在控制病毒感染和肿瘤发展方面发挥着关键作用。每个MHC I类分子由一个重链α链和一个轻链β2微球蛋白组成。重α链包含一个肽结合凹槽，该凹槽对于向细胞毒性T淋巴细胞呈递抗原至关重要。轻链β2-微球蛋白与α链以非共价键结合，对于MHC I类复合物的正确折叠、稳定性和表面表达至关重要。MHC I类表达失调或下调会导致病原体或癌细胞逃避免疫系统，这凸显了它们在疾病过程中的重要性。MHC I类（W6/32）抗体是研究人员研究抗原呈递、免疫识别和相关免疫功能的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MHC class I 抗体 (W6/32) 参考文献:

  1. HLA I 类和 II 类基因及分子的多态性和进化。  |  Little, AM. and Parham, P. 1999. Rev Immunogenet. 1: 105-23. PMID: 11256568
  2. 控制 MHC 分子与肽组装的辅助蛋白质。  |  Van Kaer, L. 2001. Immunol Res. 23: 205-14. PMID: 11444385
  3. 大鼠（Rattus norvegicus）的主要组织相容性复合体。  |  Günther, E. and Walter, L. 2001. Immunogenetics. 53: 520-42. PMID: 11685465
  4. HLA 复合体的分子遗传学。  |  Fischer, GF. and Mayr, WR. 2001. Wien Klin Wochenschr. 113: 814-24. PMID: 11732117

订购信息

产品名称产品编号规格价格数量收藏夹

MHC class I 抗体 (W6/32)

sc-32235
200 µg/ml
RMB2377.00

MHC class I (W6/32): m-IgG Fc BP-HRP 套装

sc-528444
200 µg Ab; 10 µg BP
RMB2662.00

MHC class I (W6/32): m-IgGκ BP-HRP 套装

sc-520822
200 µg Ab, 40 µg BP
RMB2662.00

MHC class I (W6/32): m-IgG2a BP-HRP 套装

sc-547101
200 µg Ab; 10 µg BP
RMB2662.00

MHC class I 抗体 (W6/32) AC

sc-32235 AC
500 µg/ml, 25% agarose
RMB3129.00

MHC class I 抗体 (W6/32) HRP

sc-32235 HRP
200 µg/ml
RMB2377.00

MHC class I 抗体 (W6/32) FITC

sc-32235 FITC
200 µg/ml
RMB2482.00

MHC class I 抗体 (W6/32) PE

sc-32235 PE
200 µg/ml
RMB2580.00

MHC class I 抗体 (W6/32) Alexa Fluor® 488

sc-32235 AF488
200 µg/ml
RMB2685.00

MHC class I 抗体 (W6/32) Alexa Fluor® 546

sc-32235 AF546
200 µg/ml
RMB2685.00

MHC class I 抗体 (W6/32) Alexa Fluor® 594

sc-32235 AF594
200 µg/ml
RMB2685.00

MHC class I 抗体 (W6/32) Alexa Fluor® 647

sc-32235 AF647
200 µg/ml
RMB2685.00

MHC class I 抗体 (W6/32) Alexa Fluor® 680

sc-32235 AF680
200 µg/ml
RMB2685.00

MHC class I 抗体 (W6/32) Alexa Fluor® 790

sc-32235 AF790
200 µg/ml
RMB2685.00

MHC class I 抗体 (W6/32) B

sc-32235 B
200 µg/ml
RMB2407.00

抗体是什么系统表达的？

Asked by: koko
感谢您的提问。MHC class I 抗体 (W6/32): sc-32235是通过杂交瘤技术生产的。 Thank you for your question. MHC class I antibody (W6/32): sc-32235 is produced through hybridoma technology.
Answered by: Technical Service SH
Date published: 2025-08-22

Bonjour, faites vous également cet anticorps en mode bulk de plusieurs mg ? En vous remerciant, Bien cordialement

Asked by: Yann
Merci pour votre question. Cet anticorps peut être fourni en vrac sur demande. Veuillez contacter directement le service technique pour prendre les dispositions nécessaires. Thank you for your question. This antibody can be supplied in bulk upon request. Please contact technical service directly to make the necessary arrangements.
Answered by: Tech Support Europe
Date published: 2025-03-07

What is the difference between W6/32 and W6/32 B? And which could be used for IP directly?

Asked by: CChange
Thank you for your question. MHC class I Antibody (W6/32) B is biotin conjugated MHC class I Antibody. MHC class I Antibody (W6/32) AC: sc-32235 AC is agarose conjugated MHC class I Antibody, can be used for IP directly.
Answered by: Technical Service SH
Date published: 2024-05-27

Is this product manufactured following some kind of quality standard such as GMP or similar? If so, which one? Thanks!

Asked by: Beñat
Thank you for your question. All our products are for research use only and not intended for diagnostic or therapeutic use.
Answered by: Tech Support Europe
Date published: 2023-11-17

How much AF 647 antibody do you use for Flow Cytometry?

Asked by: 12345678987654321
Thank you for your question. We recommend using 1 µg of fluorochrome-conjugated antibodies to 100 µl of the prepared cell suspension (equal to 1 million cells). https://www.scbt.com/resources/protocols/flow-cytometry
Answered by: Technical Support
Date published: 2020-07-08

Are the agarose conjugated antibodies covalently bound ? Do they resist to acidic elution for instance and keep attached to the agarose? Thx

Asked by: YannE
Thank you for your question. Yes, they are covalently bound via the primary amine. I do not have information specifically regarding acidic elution.
Answered by: Tech Service
Date published: 2020-01-17

Is it your W6/32 Clone from ATCC? And which type of WB could be applied with your "Anticorps MHC class I (W6/32): sc-32235", reducing/denaturing condition or non-reducing condition? Thanks

Asked by: Yang1109
Thank you for your question. We recommend reducing/denaturing conditions with this antibody for Western blotting.
Answered by: Tech Service
Date published: 2019-04-16

can we use this antibody against mouse MHC I because it is recommended against Human MHC I prtotein

Asked by: junaid
Thank you for your question. This antibody, MHC class I (W6/32): sc-32235 , has not been tested for and is not recommended for detection of mouse MHC I.
Answered by: Technical Support
Date published: 2018-01-31
  • y_2025, m_9, d_7, h_4CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_10
  • loc_zh_CN, sid_32235, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 108ms
  • QUESTIONS, PRODUCT
Rated 4 out of 5 by from Good Antibody!I cheked MHC class I expression in HK-2 cell lysates by western blot. I used it 1:500 dilution, O/N at 4 C. I got a clear band at 46kDa. I have also used it for IP with 10ug/IP and it works very well. Lot: L1522
Date published: 2024-05-23
Rated 1 out of 5 by from W6/32 DID NOT WORK FOR WBNo bands appeared at all at the designated molecular weight for MHC-I WB. Then I switched to the F-3 clone and it worked very well.
Date published: 2023-05-16
Rated 1 out of 5 by from does not workI used this antibody in WB and I wasn't able to get any signal
Date published: 2021-02-22
Rated 5 out of 5 by from FACS on HFFThis antibody works very well in Flow cytometry on HFF cells even at dilution 1:50
Date published: 2018-11-01
Rated 4 out of 5 by from Good antibody for western blotI cheked MHC class 1 expression in MCF7, MB453 and A375 cell lysates by western blot. I used it 1:500 dilution, O/N at 4 C. I got a clear band at 46kDa.
Date published: 2018-10-01
Rated 1 out of 5 by from Not good with IHCWe didn't receive a signal with IHC. We tried no demasking and demasking with pH 6 and 9. We stained human tonsils and brain (5 µm FFPE).
Date published: 2018-04-12
Rated 5 out of 5 by from Good antibody for flow cytometryI got a free sample of this antibody for a flow cytometry test and it worked really well. I used human fibroblast cell lines (Hs27) and Hela cells. The antibody concentration was 1:500.
Date published: 2017-12-28
Rated 5 out of 5 by from good antibody（1/200）WB experiment：I got a very clear band. Recommended for use！
Date published: 2017-09-07
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_17
  • loc_zh_CN, sid_32235, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 19ms
  • REVIEWS, PRODUCT
MHC class I 抗体 (W6/32) is rated 3.9 out of 5 by 17.
  • y_2025, m_9, d_7, h_4
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_17
  • loc_zh_CN, sid_32235, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 107ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。