Date published: 2026-10-24

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Met Antibody (D-4) - Western Blotting - Image 350742
Met 抗体 (D-4): sc-514148. HEK293 (A) 和 A549 (B) 全细胞裂解液中 Met 表达的 Western 印迹分析.
Met 抗体 (D-4): sc-514148. HEK293 (A) 和 A549 (B) 全细胞裂解液中 Met 表达的 Western 印迹分析.

Met 抗体 (D-4): sc-514148

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说明书
  • Met抗体(D-4)是小鼠单克隆IgG1 κ, 在20篇文献中引用，规格为200 µg/ml
  • 特异性表位图谱分析在human的Met的C-terminal细胞质域内的1375-1391氨基酸之间进行
  • Met 抗体 (D-4) 推荐用于WB, IP, IF, IHC(P) 和 ELISA，检测human 来源的 Met
  • 抗Met抗体（D-4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Met Antibody (D-4) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Met Antibody (D-4) 打包提供（请参阅下面的订购信息）。
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Met抗体（D-4）是一种IgG1 κ小鼠单克隆Met抗体（也称为Met抗体或c-Met抗体），可通过WB、IP、IF、IHC(P)和ELISA检测人类来源的Met。Met抗体（D-4）既有非偶联的抗Met抗体形式，也有多种偶联形式的抗Met抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。c-Met癌基因最初是通过在NIH/3T3细胞中转染分析从化学致癌物处理的人成骨肉瘤细胞系中分离出来的。Met原癌基因产物被鉴定为一种跨膜受体样蛋白，具有酪氨酸激酶活性，在许多组织中表达。高比例的自发性 NIH/3T3 转化体过表达 c-Met，通过转染分析，c-Met 原癌基因已被证明具有转化活性。在某些人胃癌细胞系中也观察到明显正常的 Met 蛋白的酪氨酸磷酸化。c-Met基因产物已被确定为肝细胞生长因子的细胞表面受体，肝细胞生长因子是一种纤溶酶原样蛋白，被认为是肝脏再生的体液介质。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Met 抗体 (D-4) 参考文献:

  1. 对Met受体激活的结构洞察。  |  Niemann, HH. 2011. Eur J Cell Biol. 90: 972-81. PMID: 21242015
  2. c-Met抑制剂。  |  Mughal, A., et al. 2013. Infect Agent Cancer. 8: 13. PMID: 23566349
  3. 肺癌中的microRNA和MET。  |  Brighenti, M. 2015. Ann Transl Med. 3: 68. PMID: 25992367
  4. 乳腺癌中的MET失调。  |  Minuti, G. and Landi, L. 2015. Ann Transl Med. 3: 181. PMID: 26366398
  5. 癌症中的c-Met和miRs。  |  Giglio, S. and Vecchione, A. 2015. Biomedicines. 3: 32-44. PMID: 28536397
  6. HGF和MET：从大脑发育到神经系统疾病。  |  Desole, C., et al. 2021. Front Cell Dev Biol. 9: 683609. PMID: 34179015
  7. N-糖基化调节MET的加工和信号传导。  |  Saitou, A., et al. 2022. Cancer Sci. 113: 1292-1304. PMID: 35092134
  8. 在肺癌中，MET融合蛋白作为对MET抑制剂敏感的新型治疗靶标。  |  Sun, D., et al. 2023. J Transl Med. 21: 150. PMID: 36829199
  9. 通过MET激活的Wnt凝集原药逆转癌基因驱动的NSCLC中MET介导的耐药性  |  Liu, N., et al. 2024. Adv Sci (Weinh). 11: e2400603. PMID: 38867713
  10. 以 MET 癌基因为靶点的癌症免疫疗法  |  Lombardi, AM., et al. 2024. Int J Mol Sci. 25: PMID: 38892318

订购信息

产品名称产品编号规格价格数量收藏夹

Met 抗体 (D-4)

sc-514148
200 µg/ml
CNY2422.00

Met (D-4): m-IgG Fc BP-HRP 套装

sc-531044
200 µg Ab; 10 µg BP
CNY2715.00

Met (D-4): m-IgGκ BP-HRP 套装

sc-524624
200 µg Ab, 40 µg BP
CNY2715.00

Met (D-4): m-IgG1 BP-HRP 套装

sc-544371
200 µg Ab; 20 µg BP
CNY2715.00

Met 抗体 (D-4) AC

sc-514148 AC
500 µg/ml, 25% agarose
CNY3189.00

Met 抗体 (D-4) HRP

sc-514148 HRP
200 µg/ml
CNY2422.00

Met 抗体 (D-4) FITC

sc-514148 FITC
200 µg/ml
CNY2527.00

Met 抗体 (D-4) PE

sc-514148 PE
200 µg/ml
CNY2625.00

Met 抗体 (D-4) Alexa Fluor® 488

sc-514148 AF488
200 µg/ml
CNY2738.00

Met 抗体 (D-4) Alexa Fluor® 546

sc-514148 AF546
200 µg/ml
CNY2738.00

Met 抗体 (D-4) Alexa Fluor® 594

sc-514148 AF594
200 µg/ml
CNY2738.00

Met 抗体 (D-4) Alexa Fluor® 647

sc-514148 AF647
200 µg/ml
CNY2738.00

Met 抗体 (D-4) Alexa Fluor® 680

sc-514148 AF680
200 µg/ml
CNY2738.00

Met 抗体 (D-4) Alexa Fluor® 790

sc-514148 AF790
200 µg/ml
CNY2738.00

Met (D-4) 中和勝肽

sc-514148 P
100 µg/0.5 ml
CNY519.00

Does this antibody recognize the extracellular domain of c-MET? I need to use it for immunocytochemistry and IF.

Asked by: Ssteph
Thank you for your question. This antibody is specific for an epitope mapping between amino acids 1375-1391 within a C-terminal cytoplasmic domain of Met of human origin. It is recommended and warrantied for immunocytochemistry and immunofluorescence, so it would be a great choice for this use.
Answered by: Tech Service
Date published: 2024-01-04

What application is the blocking peptide sc-514148 P appropriate for?

Asked by: cjMara
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2024-01-04
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Rated 5 out of 5 by Whey from NãoBom esse produto é recomendado pra todas as idades
Date published: 2023-10-18
Rated 5 out of 5 by Lindo from Otima funcionalidadeComprei e indico produtos de qualidades incrivelmente ótimos.
Date published: 2023-10-09
Rated 4 out of 5 by Anonymous from Análise exelente!Comprei este produto á 5 meses e funciona!! Super recomendo
Date published: 2023-10-08
Rated 3 out of 5 by JohnH from DetectableWhen testing, was detectable but signaling was weak.
Date published: 2021-11-22
Rated 2 out of 5 by Mia332 from Did not work in mouse liver lysate, I tried it witDid not work in mouse liver lysate, I tried it with 1:500 dilutions
Date published: 2018-04-12
Rated 1 out of 5 by Rutaiwan from Weak signalThis antibody (sc-514148) gives weaker signal compared to the previous antibody (sc-161) that has been terminated.
Date published: 2017-11-10
Rated 5 out of 5 by kidarime from works very well with mouse epithelial cellsI could get clear and specific band, and the major is 145kda.
Date published: 2017-10-18
Rated 5 out of 5 by tianya from Works GreatThis antibody is strong signal, low background for IHC（P）. Cytoplasmic staining of c-Met were demonstrated in most cancer cells. Very beautiful tissue staining.
Date published: 2017-10-16
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Met 抗体 (D-4) is rated 4.0 out of 5 by 13.
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