Date published: 2025-9-8
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ME1 Antibody (99.1) - Western Blotting - Image 134952
ME1 抗体 (99. 1): sc-100569. Western 印迹分析 HeLa 全细胞裂解液中 ME1 的表达.
ME1 抗体 (99. 1): sc-100569. Western 印迹分析 HeLa 全细胞裂解液中 ME1 的表达.

ME1 抗体 (99.1): sc-100569

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说明书
  • ME1抗体(99.1)是小鼠单克隆IgG1 κ, 在10篇文献中引用，规格为100 µg/ml
  • 免疫human物种的重组ME1
  • 推荐用于 mouse, rat 和 human 来源的ME1 WB, IP, IF, IHC(P) 和 ELISA检测
  • 关于如何获取ME1 (99.1): sc-100569的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRP 是 ME1 抗体 (99.1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与ME1 抗体 (99.1) 组合成套装提供。 (请参阅下面的订购信息)。

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研究信息

関連項目

ME1抗体（99.1）是一种IgG1 κ小鼠单克隆ME1抗体，可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人源的ME1。ME1抗体（99.1）可作为非偶联的抗ME1抗体形式提供。ME1（苹果酸酶1），也称为NADP-ME、MES或HUMNDME，是一种由572个氨基酸组成的细胞质蛋白，属于苹果酸酶家族。ME1在肝脏和白色脂肪组织中表达量最高，且普遍存在，作为一种NADP依赖性酶，催化S-苹果酸和NADP转化为丙酮酸、二氧化碳和NADPH（参与脂肪酸生物合成的还原剂）。ME1通过催化苹果酸的可逆氧化脱羧作用，将柠檬酸和糖酵解循环联系起来。ME1以同源四聚体形式存在，使用二价金属阳离子（如镁或锰）作为辅因子。ME1的表达受甲状腺激素水平和饮食中碳水化合物的调节，这表明ME1可能在细胞内作为管家蛋白发挥重要作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

ME1 抗体 (99.1) 参考文献:

  1. ATP 和富马酸调节人类线粒体 NAD(P)+ 依赖性苹果酸酶的分子机制。  |  Yang, Z., et al. 2002. Structure. 10: 951-60. PMID: 12121650
  2. 人类线粒体 NAD(P)+ 依赖性苹果酸酶的双辅助因子特异性和底物合作性的决定因素：谷氨酰胺的功能作用 362.  |  Hsieh, JY., et al. 2006. J Biol Chem. 281: 23237-45. PMID: 16757477
  3. 心脏和肌肉中的人类苹果酸酶：选择性分布的证据。  |  Tessarolo, D., et al. 1991. Biochem Med Metab Biol. 45: 1-5. PMID: 2015102
  4. ME1调节NADPH平衡，促进胃癌生长和转移  |  Lu, YX., et al. 2018. Cancer Res. 78: 1972-1985. PMID: 29654155
  5. ME1 磷酸化和乙酰化的动态调控影响脂质代谢和结直肠肿瘤发生  |  Zhu, Y., et al. 2020. Mol Cell. 77: 138-149.e5. PMID: 31735643
  6. 癌症生物学中的苹果酸酶 1 (ME1)：它不仅仅是中间代谢。  |  Simmen, FA., et al. 2020. J Mol Endocrinol. 65: R77-R90. PMID: 33064660
  7. USP19 通过稳定 ME1 加剧脂肪生成和结直肠癌的发生。  |  Zhu, Y., et al. 2021. Cell Rep. 37: 110174. PMID: 34965422
  8. 救治疗法通过 ME1 的上调扩增了具有高度细胞毒性和代谢适应性的 CD8+ T 细胞。  |  Gicobi, JK., et al. 2023. Sci Adv. 9: eadi2414. PMID: 37967193
  9. 调解新陈代谢：抑制苹果酸酶 1 (ME1) 可恢复肺动脉高压的内皮生物能和腺苷信号传导。  |  Rao, RJ. and Chan, SY. 2024. Circulation. 149: 1372-1374. PMID: 38648276
  10. 人肝脏中 NADP 依赖性苹果酸酶 cDNA 的克隆, 测序和功能表达。  |  González-Manchón, C., et al. 1995. Gene. 159: 255-60. PMID: 7622060
  11. 人类肿瘤细胞中细胞膜苹果酸酶的特征。  |  Loeber, G., et al. 1994. FEBS Lett. 344: 181-6. PMID: 8187880
  12. 人类细胞质苹果酸酶启动子的分子克隆和功能特征：甲状腺激素反应性。  |  González-Manchón, C., et al. 1997. DNA Cell Biol. 16: 533-44. PMID: 9174159

订购信息

产品名称产品编号规格价格数量收藏夹

ME1 抗体 (99.1)

sc-100569
100 µg/ml
RMB2504.00

ME1 (99.1): m-IgG1 BP-HRP 套装

sc-542631
100 µg Ab; 20 µg BP
RMB2662.00

Which epitope of ME-1 does this antibody recognize?

Asked by: Avinash
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2019-08-17

Can sc-100569: ME1 (99.1) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Germaine
Thank you for your question. Yes, sc-100569: ME1 (99.1) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Good performance in Western BlotWe detected a clear and specific band in protein lysates from both CaLu-6 and HCC-1937 cell lines.
Date published: 2025-05-05
Rated 5 out of 5 by from Great choice for WBGave a nice strong and clean band in WB on Hep G2 and HeLa whole cell lysate
Date published: 2017-01-26
Rated 5 out of 5 by from Perfect!We bought this antibody 2 years ago, it also can work today.
Date published: 2017-01-06
Rated 4 out of 5 by from WB publishable data with human sampleWB publishable data with human sample - SCBT Publication Review
Date published: 2015-05-19
Rated 4 out of 5 by from Produced wonderful immunoperoxidase cytoplasmicProduced wonderful immunoperoxidase cytoplasmic staining in formalin-fixed, paraffin-embedded human tonsil tissue. -SCBT QC
Date published: 2014-04-20
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ME1抗体 (99.1) is rated 4.4 out of 5 by 5.
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