Date published: 2025-9-7
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MCM7 Antibody (DCS-141) - Western Blotting - Image 356613
MCM7 抗体 (DCS-141): sc-65469. HL-60 (A) 和 L8 (B) 全细胞裂解液中 MCM7 表达的 Western 印迹分析.
MCM7 抗体 (DCS-141): sc-65469. HL-60 (A) 和 L8 (B) 全细胞裂解液中 MCM7 表达的 Western 印迹分析.

MCM7 抗体 (DCS-141): sc-65469

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说明书
  • MCM7抗体(DCS-141)是小鼠单克隆IgG1 κ, 在21篇文献中引用，规格为200 µg/ml
  • 针对全长MCM7，human起源
  • 推荐用于 mouse, rat 和 human 来源的MCM7 WB, IP, IF, IHC(P) 和 ELISA检测
  • 关于如何获取MCM7 (DCS-141): sc-65469的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 MCM7 抗体 (DCS-141) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MCM7 抗体 (DCS-141) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 MCM 抗体 包括 MCM2, MCM3, MCM4, MCM5, MCM6和MCM7

MCM7抗体（DCS-141）是一种IgG1κ小鼠单克隆MCM7抗体，可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人来源的MCM7。MCM7抗体（DCS-141）以非偶联的抗MCM7抗体形式提供。微染色体维持（MCM）蛋白家族，包括MCM2、MCM3、MCM4（Cdc21）、MCM5（Cdc46）、MCM6（Mis5）和MCM7（Cdc47），是DNA复制的调节因子，其作用在于确保复制在细胞周期中只发生一次。MCM蛋白的表达在细胞生长过程中增加，在G1至S期达到高峰。每个MCM蛋白都包含一个ATP结合基序，预计会介导ATP依赖性的双链DNA打开。MCM蛋白受E2F转录因子的调控，该因子可诱导MCM的表达，以及受蛋白激酶的调控，后者与MCM蛋白相互作用以维持细胞的复制后状态。MCM2/MCM4复合物在体外作为Cdc2/cyclin B的底物发挥作用。MCM3的裂解（可被caspase抑制剂阻止）会导致至少由MCM2-6组成的MCM复合物在凋亡期间失活。已显示由MCM4、MCM6和MCM7组成的复合物参与DNA解旋酶活性，而MCM5参与IFN-γ诱导的Stat1α转录激活。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MCM7 抗体 (DCS-141) 参考文献:

  1. 作为子宫内膜癌增殖标志物的复制 MCM7 蛋白：组织芯片和临床病理分析。  |  Li, SS., et al. 2005. Histopathology. 46: 307-13. PMID: 15720416
  2. MCM7 扩增和过表达与前列腺癌的进展有关。  |  Ren, B., et al. 2006. Oncogene. 25: 1090-8. PMID: 16247466
  3. 小染色体维护蛋白 MCM7 的失调导致了癌基因驱动的肿瘤发生。  |  Honeycutt, KA., et al. 2006. Oncogene. 25: 4027-32. PMID: 16518415
  4. MCM7 转化体的致癌活性。  |  Luo, JH. 2011. World J Clin Oncol. 2: 120-4. PMID: 21603321
  5. 细胞周期蛋白/细胞周期蛋白依赖性激酶对迷你染色体维护蛋白7（MCM7）的磷酸化会影响其在细胞周期调控中的功能。  |  Wei, Q., et al. 2013. J Biol Chem. 288: 19715-25. PMID: 23720738
  6. MCM7是弥漫型胃腺癌的预后标志物，siRNA介导的敲除可抑制其致癌功能。  |  Kang, W., et al. 2014. Oncol Rep. 31: 2071-8. PMID: 24647462
  7. MCM7 扩增和过表达通过激活 AKT1/mTOR 信号通路，促进食管鳞状细胞癌的细胞增殖、集落形成和迁移。  |  Qiu, YT., et al. 2017. Oncol Rep. 37: 3590-3596. PMID: 28498460
  8. 通过计算筛选天然化合物，鉴定以 MCM7 蛋白为靶点的潜在抗癌药物。  |  Alshahrani, MY., et al. 2021. Molecules. 26: PMID: 34641424
  9. RNF8 介导的 MCM7 多泛素化：将 CMG 螺旋酶的解体与人类细胞中的 DNA 损伤反应联系起来。  |  Sun, Q., et al. 2024. Life Sci. 353: 122912. PMID: 39004272
  10. SETD3 介导的 MCM7 组氨酸甲基化通过促进 MCM 的染色质负载来调节 DNA 复制。  |  Duan, H., et al. 2024. Sci China Life Sci.. PMID: 39455502

订购信息

产品名称产品编号规格价格数量收藏夹

MCM7 抗体 (DCS-141)

sc-65469
200 µg/ml
RMB2377.00

MCM7 (DCS-141): m-IgG Fc BP-HRP 套装

sc-526955
200 µg Ab; 10 µg BP
RMB2662.00

MCM7 (DCS-141): m-IgG1 BP-HRP 套装

sc-532328
200 µg Ab; 20 µg BP
RMB2662.00

I am using MCM7 (DCS-141): sc-65469 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: Professor Griffin
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Excellent for WB in both human and chicken cellsThe antibody was used at the dilution of 1:200. The band was specific and the signal was strong!
Date published: 2021-02-23
Rated 5 out of 5 by from a good antibody for WBThis antibody generates a clear signal at the predicted position on the WB (1:500 dilution, 40ug HeLa extract).
Date published: 2017-09-18
Rated 5 out of 5 by from Excellent for WBStrong band with 1:1,000 dilution by chemiluminescence detection (mouse tissue samples)
Date published: 2017-08-04
Rated 5 out of 5 by from Successfully immunoprecipitated MCM7 from USuccessfully immunoprecipitated MCM7 from U-2-OS and transformed ATM-deficient cells and verified via western blot. -SCBT Publication Review
Date published: 2015-05-18
Rated 5 out of 5 by from Exceptional immunoperoxidase nuclear stainingExceptional immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human tonsil tissue. -SCBT QC
Date published: 2015-04-12
Rated 5 out of 5 by from WB: works well with RECsWB: works well with RECs. -SCBT Publication Review
Date published: 2015-02-23
Rated 5 out of 5 by from Good antibody for western blot; used with MCFGood antibody for western blot; used with MCF-7, ZR-75, BT-549, MDA MB-468, MDA MB-453, MDA MB-231, T47D, SKBR-3, PMC-42DU-145 and PC-3, LNCaP cells. -SCBT Publication Review
Date published: 2015-01-27
Rated 5 out of 5 by from Positive results in COLO 320DMPositive results in COLO 320DM, H4 and HeLa whole cell lysates. -SCBT QC
Date published: 2015-01-02
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MCM7 抗体 (DCS-141) is rated 5.0 out of 5 by 8.
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