Date published: 2025-9-6
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IRF3 Antibody (SL-12) - Western Blotting - Image 394363
IRF-3 抗体 (SL-12) HRP: sc-33641 HRP. HL-60 (A), HT-1080 (B), U-937 (C), HeLa (D), K-562 (E) 和 MCF7 (F) 全细胞裂解液中 IRF-3 表达的直接 Western 印迹分析.
IRF-3 抗体 (SL-12) HRP: sc-33641 HRP. HL-60 (A), HT-1080 (B), U-937 (C), HeLa (D), K-562 (E) 和 MCF7 (F) 全细胞裂解液中 IRF-3 表达的直接 Western 印迹分析.

IRF3 抗体 (SL-12): sc-33641

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说明书
  • IRF3 抗体 SL-12 是小鼠单克隆 IgG1 κ，IRF3抗体, 在86篇文献中引用，规格为200 µg/ml
  • 针对与human IRF-3（氨基酸56-427）对应的重组IRF-3融合蛋白
  • IRF3 抗体 (SL-12) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 IRF-3
  • 抗IRF3抗体（SL-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-33641 X, 200 µg/0.1 ml)
  • 关于如何获取IRF3 (SL-12): sc-33641的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 IRF3 抗体 (SL-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与IRF3 抗体 (SL-12) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 IRF 抗体 包括 IRF-1, IRF-2, IRF-3, IRF-4, IRF-5, IRF-6, IRF-7, IRF-2BP1和IRF-2BP

IRF-3抗体（SL-12）是一种小鼠单克隆IgG1 kappa轻链抗体，旨在以极高的精确度靶向干扰素调节因子3（IRF-3）蛋白。抗IRF-3抗体（SL-12）针对与人类IRF-3（氨基酸56-427）相对应的重组IRF-3融合蛋白进行工程改造，可在多种应用中可靠地检测小鼠、大鼠和人类的IRF-3，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和石蜡包埋切片免疫组织化学（IHCP）。IRF-3，也称为IIAE7，是先天免疫反应中的一种关键转录因子，在检测病毒感染和随后激活I型干扰素（如IFN-β）方面发挥着至关重要的作用。在病毒感染时，IRF-3经历磷酸化、二聚化并转移到细胞核，在那里与特定的DNA元件结合，启动干扰素基因的转录，从而协调强大的抗病毒防御机制。了解和分析IRF-3的功能对于阐明免疫调节和人体对病原体反应的分子途径至关重要。IRF-3 (SL-12) 单克隆抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物，为研究人员提供了多种工具，用于研究IRF-3在细胞过程和疾病状态中的复杂作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IRF3 抗体 (SL-12) 参考文献:

  1. IRF-3和IRF-7在针对登革热病毒的先天抗病毒免疫中的作用。  |  Hiscott, J., et al. 1999. J Interferon Cytokine Res. 19: 1-13. PMID: 10048763
  2. 通过磷酸化控制IRF-3的激活。  |  Servant, MJ., et al. 2002. J Interferon Cytokine Res. 22: 49-58. PMID: 11846975
  3. 触发干扰素反应：IRF-3转录因子的作用。  |  Yoneyama, M., et al. 2002. J Interferon Cytokine Res. 22: 73-6. PMID: 11846977
  4. 调节IRF-3和IRF-7功能的重叠和独特机制。  |  Seth, RB., et al. 2005. Cell. 122: 669-82. PMID: 16125763
  5. 百里酚通过抑制TBK1抑制IRF-3介导的第一型干扰素的表达。  |  Sasai, M., et al. 2006. J Biochem. 139: 171-5. PMID: 16452304
  6. 负责IRF-3介导的髓样树突状细胞（mDC）中IFN-β产生的激酶复合物。  |  Solis, M., et al. 2006. Biochem Pharmacol. 72: 1469-76. PMID: 16846591
  7. 褪黑激素可降低患有牙周炎且进食高脂饮食的大鼠的IRF-3蛋白在腓肠肌中的表达，降低IL-1β和LPS血浆浓度，改善血脂状况。  |  Andersen, J., et al. 2008. Genes Immun. 9: 168-75. PMID: 18094709
  8. 生长/分化因子-5的I型和II型丝氨酸/苏氨酸激酶受体的鉴定。  |  Chen, HW., et al. 2013. J Immunol. 191: 4194-201. PMID: 24043884
  9. 病毒感染期间IRF-3依赖性和增强性靶基因。  |  Aziz, N., et al. 2018. Int J Mol Sci. 19: PMID: 29751576
  10. IRF-3和IRF-7在IFN-α基因调控和原代巨噬细胞抗肿瘤活性控制中的独特功能。  |  Jing, T., et al. 2020. J Immunol. 205: 1886-1896. PMID: 32826280

订购信息

产品名称产品编号规格价格数量收藏夹

IRF3 抗体 (SL-12)

sc-33641
200 µg/ml
RMB2377.00

IRF3 (SL-12): m-IgG Fc BP-HRP 套装

sc-526695
200 µg Ab; 10 µg BP
RMB2662.00

IRF3 (SL-12): m-IgG1 BP-HRP 套装

sc-532068
200 µg Ab; 20 µg BP
RMB2662.00

IRF3 抗体 (SL-12) X

sc-33641 X
200 µg/0.1 ml
RMB2377.00

IRF3 抗体 (SL-12) AC

sc-33641 AC
500 µg/ml, 25% agarose
RMB3129.00

IRF3 抗体 (SL-12) HRP

sc-33641 HRP
200 µg/ml
RMB2377.00

IRF3 抗体 (SL-12) FITC

sc-33641 FITC
200 µg/ml
RMB2482.00

IRF3 抗体 (SL-12) PE

sc-33641 PE
200 µg/ml
RMB2580.00

IRF3 抗体 (SL-12) Alexa Fluor® 488

sc-33641 AF488
200 µg/ml
RMB2685.00

IRF3 抗体 (SL-12) Alexa Fluor® 546

sc-33641 AF546
200 µg/ml
RMB2685.00

IRF3 抗体 (SL-12) Alexa Fluor® 594

sc-33641 AF594
200 µg/ml
RMB2685.00

IRF3 抗体 (SL-12) Alexa Fluor® 647

sc-33641 AF647
200 µg/ml
RMB2685.00

IRF3 抗体 (SL-12) Alexa Fluor® 680

sc-33641 AF680
200 µg/ml
RMB2685.00

IRF3 抗体 (SL-12) Alexa Fluor® 790

sc-33641 AF790
200 µg/ml
RMB2685.00

Does this Anti-IRF3 antibody sc-33641 detect phosphorylated or activated form of IRF3?

Asked by: Mark
Thank you for contacting Santa Cruz Biotechnology. For assistance please contact Technical support. Please call 800-457-3801 or email [email protected].
Answered by: BlakeJ
Date published: 2025-07-23

I want to perform ChIP-seq of IRF3 as done by the ENCODE team; they used your antibody sc-9082 for all their experiments. Sadly you discontinued it, and your new IRF3 AB is not validated for ChIP. Can you help me get the appropriate product from you?

Asked by: Arianna
Thank you for contacting Santa Cruz Biotechnology . For ChIP we recommend using sc-33641 X, 200 µg/0.1 ml. https://www.scbt.com/resources/protocols/chromatin-immunoprecipitation-assays
Answered by: BlakeJ
Date published: 2025-04-30

Is this antibody able to detect IRF3 dimers ?

Asked by: LeeL
Thank you for your question. We have not detected IRF-3 dimers with this antibody, however, it's possible that the antibody could detect a dimer if it was present in the sample.
Answered by: Tech Service
Date published: 2020-06-01

可以染小鼠组织免疫荧光吗

Asked by: 123mmmm
Thanks for your question. IRF-3 (SL-12) is recommended for detection by IF on mouse origin. 感谢您的问题。IRF-3 抗体 (SL-12)推荐免疫荧光方法检测小鼠来源的样本。
Answered by: Technical Support 9
Date published: 2020-05-19

You report that sc-33641 detects IRF3 of mouse and human origin, yet all your examples are human. Do you have an example showing positive WB in mouse cells/tissue, with verification it is NOT present in a KO or is induced in a TG?

Asked by: jjmaher
Thank you for your question. We do not have data in mouse cells or tissues at this time. The antibody is recommended for detection of IRF-3 of mouse, rat and human origin based on sequence identity. If the antibody does not work in any of the recommended applications or species, please contact our Technical Service Department and we would be happy to provide you with a complimentary replacement antibody, refund or credit per our product warranty.
Answered by: Technical Support
Date published: 2021-08-09

When should the TransCruz reagent sc-33641 X be used instead of the standard form sc-33641?

Asked by: jenniferc
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support 5
Date published: 2017-01-20
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Rated 1 out of 5 by from Not so good in IFFor us this ab didn't worked in IF on LLC-MK2 (dil 1:100)
Date published: 2023-06-29
Rated 5 out of 5 by from 功能很出色的 抗体多次使用，效果优秀，多次反复使用都没有问题，强于其他公司的抗体，值得大家购买和推荐。1：1500都可以做出来。多个实验都可以做出比较理想的效果。值得推荐。赞一个。
Date published: 2022-05-22
Rated 3 out of 5 by from IF(free sample) used for IF for characterization of tumor cells, No problem for signal detection
Date published: 2020-10-06
Rated 5 out of 5 by from very beautiful WBI get prospective results by using this IRF3 antibody!
Date published: 2017-12-17
Rated 5 out of 5 by from Very strong signal in WBShows a very strong signal in WB at a dilution of 1:200 (10 ug protein loaded per lane)
Date published: 2017-10-18
Rated 5 out of 5 by from work wellwe got it several months ago and it works well, we like it !
Date published: 2017-10-11
Rated 5 out of 5 by from IRF3Works great in Western Blot. Used in 5% milk powder.
Date published: 2017-10-10
Rated 4 out of 5 by from Why I can't see the nuclear translocation of IRF3?I can detect signal. But can't see any nuclear translocation for IRF3 after poly(I:C) stimulation for macrophage. I don't know why, the antibody I used for IF is 1:250.
Date published: 2017-09-19
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IRF3 抗体 (SL-12) is rated 4.6 out of 5 by 20.
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