Date published: 2026-10-20

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Integrin α5/ITGA5/CD49e Antibody (C-9) - Western Blotting - Image 375711
Integrin α5抗体 (C-9): sc-376199. 未转染 293T: sc-117752 (A), 人 Integrin α5 转染 293T: sc-158642 (B), JAR (C) 和 HUV-EC-C (D) 全细胞裂解液中 Integrin α5 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.
Integrin α5抗体 (C-9): sc-376199. 未转染 293T: sc-117752 (A), 人 Integrin α5 转染 293T: sc-158642 (B), JAR (C) 和 HUV-EC-C (D) 全细胞裂解液中 Integrin α5 表达的近红外 western 印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 所用检测试剂: m-IgGκ BP-CFL 680: sc-516180.

Integrin α5/ITGA5/CD49e 抗体 (C-9): sc-376199

3.0(8)
寫評論提問
说明书
  • Integrin α5/ITGA5/CD49e 抗体 C-9 是小鼠单克隆 IgG2b κ，Integrin α5/ITGA5/CD49e抗体, 在17篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的Integrin α5的C-terminus的氨基酸867-894之间
  • Integrin α5/ITGA5/CD49e 抗体 (C-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Integrin α5 heavy chain
  • 抗Integrin α5/ITGA5/CD49e抗体（C-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 2b BP-HRP">m-IgG2b BP-HRP和m-IgGκ BP-HRP是Integrin α5/ITGA5/CD49e Antibody (C-9) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。这些试剂现与Integrin α5/ITGA5/CD49e Antibody (C-9) 打包提供（请参阅下面的订购信息）。
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関連項目

额外Integrin抗体，包括Integrin α 1, Integrin α 11, Integrin α 2, Integrin α 3, Integrin α 4, Integrin α 5, Integrin α 6, Integrin α 7, Integrin α 8, Integrin α 9, Integrin α E, Integrin α IIb, Integrin α IIIa, Integrin α IIIb, Integrin α L和Integrin α M

Integrin α5抗体（C-9）是一种IgG2b κ小鼠单克隆Integrin alpha 5抗体（也被指定为CD49e抗体、ITGA5抗体、纤连蛋白受体alpha抗体、Integrin alpha 5抗体或Integrin alpha-F抗体），可通过蛋白质印迹、免疫沉淀、免疫荧光、免疫组织化学（P）和酶联免疫吸附试验（ELISA）检测小鼠、大鼠和人类来源的Integrin alpha 5蛋白。Integrin α5抗体（C-9）可作为非偶联的抗Integrin alpha 5抗体形式提供，也可作为多种偶联形式的抗Integrin alpha 5抗体提供，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®偶联物。整合素是由非共价结合的跨膜α和β亚基组成的异二聚体。16个α亚基和8个β亚基异二聚化，产生20多种不同的受体。大多数整合素受体结合细胞外基质的成分，包括纤连蛋白、胶原蛋白和玻连蛋白。某些整合素还可以与可溶性配体（如纤维蛋白原）或相邻细胞上的反向受体（如细胞内粘附分子（ICAMs））结合，从而导致细胞聚集。配体通过与相邻的整合素受体结合来交联或聚集整合素；受体聚集和配体占据对于激活整合素介导的反应都是必需的。除了介导细胞粘附和细胞骨架组织外，整合素还作为信号受体发挥作用。由整合素转导的信号在许多生物过程中发挥作用，包括细胞生长、分化、迁移和凋亡。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Integrin α5/ITGA5/CD49e 抗体 (C-9) 参考文献:

  1. PKCdelta和丝切蛋白的激活会影响表达整合素α5但不是其无尾突变的细胞的外周肌动蛋白重组和细胞间接触。  |  Oh, MA., et al. 2007. J Cell Sci. 120: 2717-30. PMID: 17646675
  2. 引物整合素α5可促进人间质基质细胞成骨细胞分化和成骨。  |  Hamidouche, Z., et al. 2009. Proc Natl Acad Sci U S A. 106: 18587-91. PMID: 19843692
  3. 在风湿性瓣膜病和心房颤动患者的心房中，钙网蛋白的过度表达与整合素α5和转化生长因子β1的表达相关。  |  Zhao, F., et al. 2013. Int J Cardiol. 168: 2177-85. PMID: 23465222
  4. 内皮细胞通过内源性纤连蛋白和整合素α5β1与仿生聚多巴胺涂层直接粘附。  |  Wang, JL., et al. 2013. Macromol Biosci. 13: 483-93. PMID: 23471770
  5. 转化生长因子（TGF）β1通过miR-130b增加整合素α5，促进结肠直肠癌细胞的迁移。  |  Yi, R., et al. 2016. Tumour Biol. 37: 10763-73. PMID: 26873488
  6. miR-205下调整合素α5可抑制三阴性乳腺癌的干细胞性和转移，抑制Src/Vav2/Rac1通路。  |  Xiao, Y., et al. 2018. Cancer Lett. 433: 199-209. PMID: 29964204
  7. 由于细胞外基质的沉积和细胞外基质受体活性的增强，引物整合素α5促进了人牙髓干细胞的牙源性分化。  |  Wang, H., et al. 2019. J Cell Physiol. 234: 12897-12909. PMID: 30556904
  8. 高迁移率基团核糖体结合 2 通过核因子 I 减少整合素 α5/β1 介导的肺炎克雷伯氏菌对人类肺上皮细胞的粘附。  |  Geng, F., et al. 2020. Microbiol Immunol. 64: 825-834. PMID: 33034909
  9. 通过靶向胰腺星状细胞中的整合素α5，对胰腺癌异种移植的SPECT/CT成像进行初步研究。  |  Wang, T., et al. 2021. J Cancer. 12: 1729-1733. PMID: 33613761
  10. PRELP通过结合整合素α5降低细胞硬度和粘附性，促进结肠直肠癌细胞的生长和转移。  |  Gui, Y., et al. 2024. Exp Cell Res. 441: 114151. PMID: 38992455

订购信息

产品名称产品编号规格价格数量收藏夹

Integrin α5/ITGA5/CD49e 抗体 (C-9)

sc-376199
200 µg/ml
CNY2422.00

Integrin α5/ITGA5/CD49e (C-9): m-IgGκ BP-HRP 套装

sc-522871
200 µg Ab, 40 µg BP
CNY2715.00

Integrin α5/ITGA5/CD49e (C-9): m-IgG2b BP-HRP 套装

sc-549076
200 µg Ab; 10 µg BP
CNY2715.00

Integrin α5/ITGA5/CD49e 抗体 (C-9) AC

sc-376199 AC
500 µg/ml, 25% agarose
CNY3189.00

Integrin α5/ITGA5/CD49e 抗体 (C-9) HRP

sc-376199 HRP
200 µg/ml
CNY2422.00

Integrin α5/ITGA5/CD49e 抗体 (C-9) FITC

sc-376199 FITC
200 µg/ml
CNY2527.00

Integrin α5/ITGA5/CD49e 抗体 (C-9) PE

sc-376199 PE
200 µg/ml
CNY2625.00

Integrin α5/ITGA5/CD49e 抗体 (C-9) Alexa Fluor® 488

sc-376199 AF488
200 µg/ml
CNY2738.00

Integrin α5/ITGA5/CD49e 抗体 (C-9) Alexa Fluor® 546

sc-376199 AF546
200 µg/ml
CNY2738.00

Integrin α5/ITGA5/CD49e 抗体 (C-9) Alexa Fluor® 594

sc-376199 AF594
200 µg/ml
CNY2738.00

Integrin α5/ITGA5/CD49e 抗体 (C-9) Alexa Fluor® 647

sc-376199 AF647
200 µg/ml
CNY2738.00

Integrin α5/ITGA5/CD49e 抗体 (C-9) Alexa Fluor® 680

sc-376199 AF680
200 µg/ml
CNY2738.00

Integrin α5/ITGA5/CD49e 抗体 (C-9) Alexa Fluor® 790

sc-376199 AF790
200 µg/ml
CNY2738.00

Integrin α5/ITGA5/CD49e (C-9) 中和勝肽

sc-376199 P
100 µg/0.5 ml
CNY519.00

What application is the blocking peptide sc-376199 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by Lizzie W from Did not stain specificallyThis antibody did not stain specifically in my flow cytometry assay. I was unable to identify my target population. Increasing concentrations only increased background.
Date published: 2022-09-12
Rated 1 out of 5 by Markov from No signalWe tried this Ab in hela cells but we did not detect any signal
Date published: 2020-12-02
Rated 4 out of 5 by Anais85 from Works in WB for human cellsI tested this antibody for WB in RCS cells (rat chondrosarcoma), SaOS-2 cells and primary human amniocytes. It gives a nice strong band in human cells but nothing in rat cells.
Date published: 2017-10-27
Rated 5 out of 5 by ZNAN from good featureswe used this antibody to detect the expression of integrin in glioma cell lysate by western blot
Date published: 2017-08-14
Rated 5 out of 5 by Just15 from Works well for WBWorks well for WB. Used the antibody at 1:1000 dilution for MDA-MB-231 cell lysate.
Date published: 2017-06-07
Rated 1 out of 5 by kmxu from it did not work for WBit did not pick any right band up but one between 75 and 100KDa in the lysates derived from mousdr macrophages
Date published: 2017-05-09
Rated 3 out of 5 by JL from Positive antigen detection by WB in transfectedPositive antigen detection by WB in transfected lysates. -SCBT QC
Date published: 2014-12-27
Rated 4 out of 5 by SF from Immunoperoxidase staining of formalin fixedImmunoperoxidase staining of formalin fixed, paraffin-embedded human placenta tissue shows membrane and cytoplasmic staining of trophoblastic cells with sc-376199. -SCBT QC
Date published: 2014-11-19
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Integrin α5/ITGA5/CD49e 抗体 (C-9) is rated 3.0 out of 5 by 8.
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