Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
Integrin α4/ITGA4/CD49d Antibody (C-2) - Western Blotting - Image 374852
Integrin α4抗体 (C-2): sc-365569. GA-10 (A), CCRF-CEM (B), SUP-T1 (C), HEL 92. 1. 7 (D), NCI-H929 (E) 和 HL-60 (F) 全细胞裂解液中 Integrin α4 表达的 Western 印迹分析.
Integrin α4/ITGA4/CD49d Antibody (C-2) - Western Blotting - Image 374852
Integrin α4抗体 (C-2): sc-365569. GA-10 (A), CCRF-CEM (B), SUP-T1 (C), HEL 92. 1. 7 (D), NCI-H929 (E) 和 HL-60 (F) 全细胞裂解液中 Integrin α4 表达的 Western 印迹分析.
Integrin α4/ITGA4/CD49d Antibody (C-2) - Western Blotting - Image 142598
Integrin α4 (C-2): sc-365569. Western 印迹分析 Jurkat (A) 和 MOLT-4 (B) 全细胞裂解液中 Integrin α4 的表达.
Integrin α4抗体 (C-2): sc-365569. GA-10 (A), CCRF-CEM (B), SUP-T1 (C), HEL 92. 1. 7 (D), NCI-H929 (E) 和 HL-60 (F) 全细胞裂解液中 Integrin α4 表达的 Western 印迹分析.

Integrin α4/ITGA4/CD49d 抗体 (C-2): sc-365569

4.2(5)
寫評論提問

说明书
  • Integrin α4/ITGA4/CD49d抗体(C-2)是小鼠单克隆IgG1 κ, 在2篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列796-1005位于human物种的Integrin α4的C-terminus附近
  • Integrin α4/ITGA4/CD49d 抗体 (C-2) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 Integrin α4
  • 抗Integrin α4/ITGA4/CD49d抗体（C-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Integrin α4/ITGA4/CD49d (C-2): sc-365569的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是Integrin α4/ITGA4/CD49d Antibody (C-2) for WB applications. 的首选辅助检测试剂，这些试剂现与Integrin α4/ITGA4/CD49d Antibody (C-2) 打包提供（请参阅下面的订购信息）。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

额外Integrin抗体，包括Integrin α 1, Integrin α 11, Integrin α 2, Integrin α 3, Integrin α 4, Integrin α 5, Integrin α 6, Integrin α 7, Integrin α 8, Integrin α 9, Integrin α E, Integrin α IIb, Integrin α IIIa, Integrin α IIIb, Integrin α L和Integrin α M

整合素α4/ITGA4/CD49d抗体（C-2）是一种IgG1 κ小鼠单克隆整合素α4抗体，可通过WB、IP、IF和ELISA检测人源整合素α4。整合素α4/ITGA4/CD49d抗体（C-2）可作为非偶联抗整合素α4抗体形式，也可作为多种偶联形式的抗整合素α4抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。整合素是由非共价结合的跨膜α和β亚基组成的异二聚体。16个α和8个β亚基异二聚化产生20多种不同的受体。大多数整合素受体结合细胞外基质的成分，包括纤维连接蛋白、胶原蛋白和玻连蛋白。某些整合素还可以结合可溶性配体，如纤维蛋白原，或结合相邻细胞上的反向受体，如细胞间粘附分子（ICAMs），导致细胞聚集。配体通过结合相邻的整合素受体来交联或聚集整合素；受体聚集和配体占据对于激活整合素介导的反应都是必需的。除了介导细胞粘附和细胞骨架组织外，整合素还作为信号受体发挥作用。由整合素转导的信号在许多生物过程中发挥作用，包括细胞生长、分化、迁移和凋亡。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Integrin α4/ITGA4/CD49d 抗体 (C-2) 参考文献:

  1. 人类胃癌细胞中整合素α4基因的异常甲基化。  |  Park, J., et al. 2004. Oncogene. 23: 3474-80. PMID: 14990990
  2. 新型促凋亡受体 NG2 通过 PKCalpha 依赖性抑制 FAK 磷酸化来对抗整合素 alpha4，从而介导 anoikis。  |  Joo, NE., et al. 2008. Cell Death Differ. 15: 899-907. PMID: 18292781
  3. DcR3通过增加整合素α4来保护THP-1巨噬细胞免于凋亡。  |  Tateishi, K., et al. 2009. Biochem Biophys Res Commun. 389: 593-8. PMID: 19748482
  4. 整合素α4影响前脂肪细胞和干细胞在合成聚合物上的不同粘附。  |  Hoss, M., et al. 2013. J Tissue Eng Regen Med. 7: 312-23. PMID: 22318983
  5. 整合素α4胞质尾部及其与paxillin相互作用的核磁共振结构。  |  Chua, GL., et al. 2013. PLoS One. 8: e55184. PMID: 23383101
  6. βI结构域中心与α1/α7螺旋之间的疏水接触对于整合素α4/β7的低亲和力状态至关重要。  |  Liu, J., et al. 2014. FEBS J. 281: 2915-26. PMID: 24802248
  7. 整合素α4信号抑制剂JK273在非小细胞肺癌NCI-H460细胞中的抗肿瘤作用  |  Lu, TN., et al. 2017. Biochem Biophys Res Commun. 491: 355-360. PMID: 28728840
  8. 大鼠间充质干细胞上的整合素α4过表达可增强转运并减少颅内注射后的脑栓塞。  |  Cui, LL., et al. 2017. Stroke. 48: 2895-2900. PMID: 28916665
  9. PSCA/PGRN-NF-κB-Integrin-α4轴促进前列腺癌细胞粘附到骨髓内皮并增强转移潜力。  |  Zhao, Z., et al. 2020. Mol Cancer Res. 18: 501-513. PMID: 31722969
  10. 整合素α4上调激活了刺猬途径，从而促进砷和苯并[α]芘共同暴露诱导的癌症干细胞样性质和肿瘤发生。  |  Xie, J., et al. 2020. Cancer Lett. 493: 143-155. PMID: 32860851

订购信息

产品名称产品编号规格价格数量收藏夹

Integrin α4/ITGA4/CD49d 抗体 (C-2)

sc-365569
200 µg/ml
RMB2377.00

Integrin α4/ITGA4/CD49d (C-2): m-IgG Fc BP-HRP 套装

sc-527381
200 µg Ab; 10 µg BP
RMB2662.00

Integrin α4/ITGA4/CD49d (C-2): m-IgG1 BP-HRP 套装

sc-532754
200 µg Ab; 20 µg BP
RMB2662.00

Integrin α4/ITGA4/CD49d 抗体 (C-2) AC

sc-365569 AC
500 µg/ml, 25% agarose
RMB3129.00

Integrin α4/ITGA4/CD49d 抗体 (C-2) HRP

sc-365569 HRP
200 µg/ml
RMB2377.00

Integrin α4/ITGA4/CD49d 抗体 (C-2) FITC

sc-365569 FITC
200 µg/ml
RMB2482.00

Integrin α4/ITGA4/CD49d 抗体 (C-2) PE

sc-365569 PE
200 µg/ml
RMB2580.00

Integrin α4/ITGA4/CD49d 抗体 (C-2) Alexa Fluor® 488

sc-365569 AF488
200 µg/ml
RMB2685.00

Integrin α4/ITGA4/CD49d 抗体 (C-2) Alexa Fluor® 546

sc-365569 AF546
200 µg/ml
RMB2685.00

Integrin α4/ITGA4/CD49d 抗体 (C-2) Alexa Fluor® 594

sc-365569 AF594
200 µg/ml
RMB2685.00

Integrin α4/ITGA4/CD49d 抗体 (C-2) Alexa Fluor® 647

sc-365569 AF647
200 µg/ml
RMB2685.00

Integrin α4/ITGA4/CD49d 抗体 (C-2) Alexa Fluor® 680

sc-365569 AF680
200 µg/ml
RMB2685.00

Integrin α4/ITGA4/CD49d 抗体 (C-2) Alexa Fluor® 790

sc-365569 AF790
200 µg/ml
RMB2685.00

What blocking solution should I use with Integrin α4 (C-2): sc-365569 monoclonal antibody for Western blot?

Asked by: Trav11
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335). Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-02-28
  • y_2025, m_8, d_31, h_8CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_1
  • loc_zh_CN, sid_365569, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 106ms
  • QUESTIONS, PRODUCT
Rated 1 out of 5 by from Didnot work for WBdid not work for western - samples were human cells in culture
Date published: 2017-11-21
Rated 5 out of 5 by from good featurewe used this antibody to detect the expression of integrin in glioma cell lysate by western blot
Date published: 2017-09-25
Rated 5 out of 5 by from Works very wellI used this antibody to detect ITGA4 in mouse bone marrow derived macrophages, and it picks up a simple right band!
Date published: 2017-04-26
Rated 5 out of 5 by from Highly potent and fairly specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was very strong detection of the ~150 kDa target protein in a HeLa/Jurkat cell lysate mix, but the target was not detected in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested or in the HeLa/Jurkat cell lysate mix, except for a medium-strong immunoreactive 36 kDa protein found in both mice and humans.
Date published: 2016-11-10
Rated 5 out of 5 by from Western blot analysis shows Integrin 4 expressionWestern blot analysis shows Integrin 4 expression in Jurkat (A) and MOLT-4 (B) whole cell lysates. -SCBT QC
Date published: 2014-01-27
  • y_2025, m_8, d_31, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_5
  • loc_zh_CN, sid_365569, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 18ms
  • REVIEWS, PRODUCT
Integrin α4/ITGA4/CD49d 抗体 (C-2) is rated 4.2 out of 5 by 5.
  • y_2025, m_8, d_31, h_8
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_5
  • loc_zh_CN, sid_365569, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 121ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。