Date published: 2025-9-6

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Integrin α4/ITGA4/CD49d 抗体 (A-7): sc-365209

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说明书
  • Integrin α4/ITGA4/CD49d抗体(A-7)是小鼠单克隆IgG2b κ, 在8篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列796-1005位于human物种的Integrin α4的C-terminus附近
  • Integrin α4/ITGA4/CD49d 抗体 (A-7) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 Integrin α4
  • 抗Integrin α4/ITGA4/CD49d抗体(A-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Integrin α4/ITGA4/CD49d (A-7): sc-365209免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前,我们还没有完成Integrin α4/ITGA4/CD49d 抗体 (A-7)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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関連項目

整合素α4/ITGA4/CD49d抗体(A-7)是一种IgG2b κ小鼠单克隆整合素α4抗体,可通过WB、IP、IF、IHC(P)和ELISA检测人源整合素α4。整合素α4/ITGA4/CD49d抗体(A-7)可作为非偶联抗整合素α4抗体形式,也可作为多种偶联形式的抗整合素α4抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。整合素是由非共价结合的跨膜α和β亚基组成的异二聚体。16个α和8个β亚基异二聚化产生20多种不同的受体。大多数整合素受体结合细胞外基质的成分,包括纤维连接蛋白、胶原蛋白和玻连蛋白。某些整合素还可以结合可溶性配体,如纤维蛋白原,或结合相邻细胞上的反向受体,如细胞间粘附分子(ICAMs),导致细胞聚集。配体通过结合相邻的整合素受体来交联或聚集整合素;受体聚集和配体占据对于激活整合素介导的反应都是必需的。除了介导细胞粘附和细胞骨架组织外,整合素还作为信号受体发挥作用。由整合素转导的信号在许多生物过程中发挥作用,包括细胞生长、分化、迁移和凋亡。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Integrin α4/ITGA4/CD49d 抗体 (A-7) 参考文献:

  1. 整合素α4和整合素α5/alphav在调节人口腔鳞癌细胞对纤维粘连蛋白的V区和肝素结合域的运动性方面的功能差异。  |  Zhang, Y., et al. 2004. Exp Cell Res. 295: 48-58. PMID: 15051489
  2. VLA-4 整合素 alpha 4 亚基裂解的功能和结构分析。  |  Teixidó, J., et al. 1992. J Biol Chem. 267: 1786-91. PMID: 1730718
  3. 整合素 VLA-4 在淋巴造血中发挥作用的证据。  |  Miyake, K., et al. 1991. J Exp Med. 173: 599-607. PMID: 1997648
  4. α-4整合素基因启动子的特征。  |  Rosen, GD., et al. 1991. Proc Natl Acad Sci U S A. 88: 4094-8. PMID: 2034655
  5. 早期植入过程中,缺氧引起的整合素α4表达动态变化与滋养细胞侵袭有关。  |  Na, KH., et al. 2012. J Cell Biochem. 113: 685-94. PMID: 21976114
  6. 整合素αv介导收缩能力,而整合素α4则在生理拉伸条件下通过FAK通路调节人膀胱平滑肌细胞的增殖。  |  Luo, DY., et al. 2013. J Urol. 190: 1421-9. PMID: 23587631
  7. VLA-4 α 4 亚基的主要结构:与其他整合素的同源性以及可能的细胞粘附功能。  |  Takada, Y., et al. 1989. EMBO J. 8: 1361-8. PMID: 2788572
  8. 循环中的整合素α4+组3先天淋巴细胞与2型糖尿病的严重程度呈负相关。  |  Zhuang, YP., et al. 2023. Int Immunopharmacol. 119: 110237. PMID: 37121112
  9. Alpha 4整合素可将病毒激活的CD8+T细胞导向感染部位。  |  Christensen, JP., et al. 1995. J Immunol. 154: 5293-301. PMID: 7537304
  10. H-2Kb 基因转染的肿瘤细胞对内皮细胞和基质蛋白的粘附力降低。  |  Lauri, D., et al. 1993. Br J Cancer. 68: 862-7. PMID: 7692918
  11. 可溶性因子与整合素介导的细胞锚定在控制细胞生长和分化中的合作。  |  Juliano, R. 1996. Bioessays. 18: 911-7. PMID: 8939069
  12. 小鼠 VCAM-1 在体外和体内不同炎症模型中的表达:与单核细胞迁移的相关性。  |  Henseleit, U., et al. 1995. Exp Dermatol. 4: 249-56. PMID: 9432255

订购信息

产品名称产品编号规格价格数量收藏夹

Integrin α4/ITGA4/CD49d 抗体 (A-7)

sc-365209
200 µg/ml
RMB2377.00

Integrin α4/ITGA4/CD49d 抗体 (A-7) AC

sc-365209 AC
500 µg/ml, 25% agarose
RMB3129.00

Integrin α4/ITGA4/CD49d 抗体 (A-7) HRP

sc-365209 HRP
200 µg/ml
RMB2377.00

Integrin α4/ITGA4/CD49d 抗体 (A-7) FITC

sc-365209 FITC
200 µg/ml
RMB2482.00

Integrin α4/ITGA4/CD49d 抗体 (A-7) PE

sc-365209 PE
200 µg/ml
RMB2580.00

Integrin α4/ITGA4/CD49d 抗体 (A-7) Alexa Fluor® 488

sc-365209 AF488
200 µg/ml
RMB2685.00

Integrin α4/ITGA4/CD49d 抗体 (A-7) Alexa Fluor® 546

sc-365209 AF546
200 µg/ml
RMB2685.00

Integrin α4/ITGA4/CD49d 抗体 (A-7) Alexa Fluor® 594

sc-365209 AF594
200 µg/ml
RMB2685.00

Integrin α4/ITGA4/CD49d 抗体 (A-7) Alexa Fluor® 647

sc-365209 AF647
200 µg/ml
RMB2685.00

Integrin α4/ITGA4/CD49d 抗体 (A-7) Alexa Fluor® 680

sc-365209 AF680
200 µg/ml
RMB2685.00

Integrin α4/ITGA4/CD49d 抗体 (A-7) Alexa Fluor® 790

sc-365209 AF790
200 µg/ml
RMB2685.00

I am using Integrin α4 (A-7): sc-365209 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: jerojero
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from Good staining!I've stained FFPE from human reactive tonsils and lymph nodes. The stain is bright in stromal cells at the dilution 1:400-1:600 using as antigen retrieval TRIS-EDTA buffer pH 9.0 (microwave 25').
Date published: 2023-02-17
Rated 5 out of 5 by from Nice antibody for Integrin expressionWe used this antibody for western blot in order to detect Integrin expression. We got positive bands shown up at the expected molecular weight in Jurkat cells.
Date published: 2017-01-27
Rated 5 out of 5 by from Immunoperoxidase staining of formalin fixedImmunoperoxidase staining of formalin fixed, paraffin-embedded human lymph node tissue shows membrane and cytoplasmic staining of cells in germinal and non-germinal centers. -SCBT QC
Date published: 2014-01-30
Rated 5 out of 5 by from StrongStrong, specific signal in western blot analysis of Integrin 4 expression in Jurkat whole cell lysate. -SCBT QC
Date published: 2013-11-11
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Integrin α4/ITGA4/CD49d 抗体 (A-7) is rated 4.8 out of 5 by 4.
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