Date published: 2025-9-8
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IDO Antibody (H-11) - Western Blotting - Image 54624
IDO 抗体 (H-11): sc-137012. 非转染: sc-117752 (A) 和小鼠 IDO 转染: sc-120945 (B) 293T 全细胞裂解液中 IDO 表达的 Western 印迹分析.
IDO 抗体 (H-11): sc-137012. 非转染: sc-117752 (A) 和小鼠 IDO 转染: sc-120945 (B) 293T 全细胞裂解液中 IDO 表达的 Western 印迹分析.

IDO 抗体 (H-11): sc-137012

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说明书
  • IDO抗体(H-11)是小鼠单克隆IgG1 κ, 在24篇文献中引用，规格为200 µg/ml
  • 免疫mouse物种的IDO的氨基酸1-80
  • 抗-IDO 抗体 (H-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 IDO
  • 抗IDO抗体（H-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IDO (H-11): sc-137012的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRP 是 IDO 抗体 (H-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与IDO 抗体 (H-11) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 IDO 抗体包括 INDOL1

IDO抗体（H-11）是一种IgG1 κ小鼠单克隆IDO抗体，可通过WB、IP、IF、IHC（P）和ELISA检测小鼠、大鼠和人源IDO。IDO抗体（H-11）既可以是非偶联的抗IDO抗体，也可以是多种偶联形式的抗IDO抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。色氨酸2,3-双加氧酶（IDO）是一种IFN-γ可诱导的酶，催化必需氨基酸L-色氨酸降解为N-甲酰犬尿氨酸。编码人IDO的基因定位于8p12-p11染色体。IDO也称为INDO，是免疫应答的重要调节剂，可保护同种异体概念免受同种异体反应性母体淋巴细胞的影响。IDO介导了与人PBLs共培养的HeLa细胞的有趣抑制作用。在HeLa细胞存在下，ILN-2诱导的PBL增殖应答减弱，而IDO抑制剂则消除了这种作用。流式细胞仪分析表明，成熟和未成熟的CD123阳性树突状细胞均使用IDO抑制T细胞活性。与T细胞共培养的IDO转染细胞可减少T细胞增殖。另外，供体T细胞的过继转移减少了IDO转基因小鼠中供体T细胞的数量。IDO的药理学或遗传学操作可能对于抑制不期望的T细胞应答是有用的。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

IDO 抗体 (H-11)

sc-137012
200 µg/ml
$316.00

IDO (H-11): m-IgG1 BP-HRP 套装

sc-531669
200 µg Ab; 20 µg BP
$354.00

IDO 抗体 (H-11) AC

sc-137012 AC
500 µg/ml, 25% agarose
$416.00

IDO 抗体 (H-11) HRP

sc-137012 HRP
200 µg/ml
$316.00

IDO 抗体 (H-11) FITC

sc-137012 FITC
200 µg/ml
$330.00

IDO 抗体 (H-11) PE

sc-137012 PE
200 µg/ml
$343.00

IDO 抗体 (H-11) Alexa Fluor® 488

sc-137012 AF488
200 µg/ml
$357.00

IDO 抗体 (H-11) Alexa Fluor® 546

sc-137012 AF546
200 µg/ml
$357.00

IDO 抗体 (H-11) Alexa Fluor® 594

sc-137012 AF594
200 µg/ml
$357.00

IDO 抗体 (H-11) Alexa Fluor® 647

sc-137012 AF647
200 µg/ml
$357.00

IDO 抗体 (H-11) Alexa Fluor® 680

sc-137012 AF680
200 µg/ml
$357.00

IDO 抗体 (H-11) Alexa Fluor® 790

sc-137012 AF790
200 µg/ml
$357.00

Is IDO (H-11): sc-137012 available directly conjugated to HRP?

Asked by: jenniferc
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at [email protected] Alternatively, consider using IDO (mIDO-48): sc-53978, which is available conjugated to agarose, HRP, FITC, PE, and more. (https://www.scbt.com/scbt/product/ido-antibody-mido-48)
Answered by: Technical Support 15
Date published: 2017-01-19
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Rated 4 out of 5 by from Good resultsI tried small size of SC-137012 antibody via Western Blot
Date published: 2021-07-07
Rated 1 out of 5 by from bad antibodyThe protein detected by this antibody does not seem to be IDO1
Date published: 2019-02-19
Rated 1 out of 5 by from Poor performanceOur lab received a free sample of this antibody. Multiple attempts to look at IDO levels in human primary macrophages failed. Using an alternate antibody from another source revealed strong expression of IDO with the same samples.
Date published: 2018-02-12
Rated 5 out of 5 by from Excellent antibody for WBWe've got strong bands when using this antibody to detect IDO in mouse hippocampus lysate sample.
Date published: 2017-11-02
Rated 5 out of 5 by from We analyze in western blotGreat antibody, specific western blot data, we will continue to purchase
Date published: 2017-05-20
Rated 5 out of 5 by from Strong WBVery nice western blot data; strong signal, low background
Date published: 2017-02-02
Rated 5 out of 5 by from Excellent!This antibody works great for western blot analysis of IDO expression in non-transfected: sc-117752 and mouse IDO transfected: sc-120945 293T whole cell lysates.
Date published: 2017-01-11
Rated 5 out of 5 by from clear WB band in human M1 macrophagesPeripheral monocytes derived macrophages were stimulated by LPS and IFN-g toward M1 type, its lysate showed clear positive IDO band compared with the control which was Peripheral monocytes derived macrophages without any stimulation.
Date published: 2016-11-10
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IDO 抗体 (H-11) is rated 4.2 out of 5 by 11.
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