Date published: 2025-9-8
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IDO Antibody (mIDO-48) - Western Blotting - Image 17790
IDO 抗体 (mIDO-48): sc-53978. 对 HeLa (A) 和经 IFN-γ 处理的 RAW 264. 7 (B) 全细胞裂解液及小鼠胎盘组织提取物 (C) 中 IDO 的表达进行 Western 印迹分析.
IDO 抗体 (mIDO-48): sc-53978. 对 HeLa (A) 和经 IFN-γ 处理的 RAW 264. 7 (B) 全细胞裂解液及小鼠胎盘组织提取物 (C) 中 IDO 的表达进行 Western 印迹分析.

IDO 抗体 (mIDO-48): sc-53978

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说明书
  • IDO 抗体 mIDO-48 是大鼠单克隆 IgG2b，IDO抗体, 在22篇文献中引用，规格为200 µg/ml
  • 针对全长重组蛋白IDO的抗体，来源于mouse
  • IDO 抗体 (mIDO-48) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 IDO
  • 抗IDO抗体（mIDO-48）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IDO (mIDO-48): sc-53978的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成IDO 抗体 (mIDO-48)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 IDO 抗体包括 INDOL1

IDO 抗体（mIDO-48）是一种大鼠单克隆 IgG2b 抗体，可通过免疫印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附试验（ELISA）等多种应用检测小鼠、大鼠和人类样本中的吲哚胺 2,3-二氧化酶（IDO）。抗吲哚抗体（mIDO-48）有非共轭型和多种共轭型，如琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和各种 Alexa Fluor® 共轭物。吲哚胺 2,3-二氧化酶在免疫调节中发挥着至关重要的作用，它催化必需氨基酸 L-色氨酸降解为 N-甲酰犬尿氨酸，这一过程对调节 T 细胞反应和维持免疫耐受性至关重要。IDO 的活性在妊娠期尤为重要，因为 IDO 通过创造局部免疫抑制环境，帮助保护发育中的胎儿免受母体免疫排斥反应的影响。此外，IDO 的表达还能抑制 T 细胞的增殖，这对癌症免疫疗法和自身免疫性疾病都有影响，因此 IDO 是一个重要的治疗干预靶点。编码人类 IDO 的基因位于染色体 8p12-p11，通过药物或基因手段调节 IDO 可为控制各种临床环境中不良 T 细胞反应提供策略。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IDO 抗体 (mIDO-48) 参考文献:

  1. 表达吲哚胺-2,3-二氧化酶的细胞可抑制 T 细胞反应。  |  Mellor, AL., et al. 2002. J Immunol. 168: 3771-6. PMID: 11937528
  2. 与外周血淋巴细胞共培养的 HeLa 细胞通过上调吲哚胺-2,3-二氧酶的活性获得免疫抑制表型。  |  Logan, GJ., et al. 2002. Immunology. 105: 478-87. PMID: 11985668
  3. 人γ干扰素诱导的吲哚胺 2,3-二氧化酶 cDNA 的分子克隆, 测序和表达。  |  Dai, W. and Gupta, SL. 1990. Biochem Biophys Res Commun. 168: 1-8. PMID: 2109605
  4. 炎症中的IDO-GCN2和自噬。  |  McGaha, TL. 2015. Oncotarget. 6: 21771-2. PMID: 26317232
  5. 肿瘤微环境中的IDO：炎症、反调节和耐受性。  |  Munn, DH. and Mellor, AL. 2016. Trends Immunol. 37: 193-207. PMID: 26839260
  6. IDO激活、炎症和肌肉骨骼疾病。  |  Ogbechi, J., et al. 2020. Exp Gerontol. 131: 110820. PMID: 31884118
  7. 癌症中的IDO/喹啉途径：可能的治疗方法。  |  Abd El-Fattah, EE. 2022. J Transl Med. 20: 347. PMID: 35918736
  8. 吲哚胺2,3-双加氧酶（IDO）抑制剂和癌症免疫疗法。  |  Fujiwara, Y., et al. 2022. Cancer Treat Rev. 110: 102461. PMID: 36058143
  9. 参与炎症诱发疾病的发病机制和治疗的 IDO 活性。  |  Reininghaus, E., et al. 2022. Curr Top Med Chem. 22: 2106. PMID: 36482738
  10. CD58 改变通过诱导弥漫大 B 细胞淋巴瘤中的 PDL1 和 IDO 来控制抗肿瘤免疫反应  |  Xu, X., et al. 2024. Cancer Res. 84: 2123-2140. PMID: 38635903
  11. 通过荧光原位杂交法确定吲哚胺2,3-二氧酶基因（INDO）在染色体8p12-->p11上的定域。  |  Najfeld, V., et al. 1993. Cytogenet Cell Genet. 64: 231-2. PMID: 8404046
  12. 通过色氨酸分解预防同种异体胎儿排斥。  |  Munn, DH., et al. 1998. Science. 281: 1191-3. PMID: 9712583

订购信息

产品名称产品编号规格价格数量收藏夹

IDO 抗体 (mIDO-48)

sc-53978
200 µg/ml
$316.00

IDO 抗体 (mIDO-48) AC

sc-53978 AC
500 µg/ml, 25% agarose
$416.00

IDO 抗体 (mIDO-48) HRP

sc-53978 HRP
200 µg/ml
$316.00

IDO 抗体 (mIDO-48) FITC

sc-53978 FITC
200 µg/ml
$330.00

IDO 抗体 (mIDO-48) PE

sc-53978 PE
200 µg/ml
$343.00

IDO 抗体 (mIDO-48) Alexa Fluor® 488

sc-53978 AF488
200 µg/ml
$357.00

IDO 抗体 (mIDO-48) Alexa Fluor® 546

sc-53978 AF546
200 µg/ml
$357.00

IDO 抗体 (mIDO-48) Alexa Fluor® 594

sc-53978 AF594
200 µg/ml
$357.00

IDO 抗体 (mIDO-48) Alexa Fluor® 647

sc-53978 AF647
200 µg/ml
$357.00

IDO 抗体 (mIDO-48) Alexa Fluor® 680

sc-53978 AF680
200 µg/ml
$357.00

IDO 抗体 (mIDO-48) Alexa Fluor® 790

sc-53978 AF790
200 µg/ml
$357.00

吉田と申します、質問失礼いたします。 マウスの細胞から抽出したタンパクをウエスタンブロッティングで解析するときにこちらの抗体を使用しております。この結果、50kと42kに日本のバンドが得られ、見本のようになりませんでした。この原因について関連がありそうな情報がありましたら教えていただけませんでしょうか。また、こちらの見本の抗体の希釈倍率など実験条件が知りたいです。 どうぞよろしくお願いいたします。

Asked by: yoshiko
Thank you for your question. The molecular weight size of the expression may vary from sample to sample. Please refer to the references for your sample. The recommended dilution ratio is 1:100. ご質問ありがとうございます。発現の分子量サイズはサンプルによって異なる場合があります。お手持ちのサンプルの文献をご参照ください。推奨希釈倍率は1:100です。
Answered by: Jiajun Z
Date published: 2025-08-07

How does this Ab work for Human cell lines?

Asked by: AsPa
Thank you for your question. Yes, IDO Antibody (mIDO-48): sc-53978 is recommended for detection of IDO of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA.
Answered by: Tech Support Europe
Date published: 2025-02-17

I noticed that sc-53978 is suitable for IHC, and i wondered that whether acidic or alkaline antigen retrieval solution is appropriate? Or maybe a protocol for IHC of sc-53978?

Asked by: zhw123
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-08-30

What is exact dilution

Asked by: Kaneez Fatima
Thank you for your question! This antibody is supplied at 200 µg in 1 ml buffer. Dilution recommendations for different assays can be found in the datasheet available on the product page. The actual optimum dilution for your particular experimental setup and sample will have to be determined experimentally.
Answered by: Tech Support Europe
Date published: 2021-07-29

Whats the difference between this antibody and the IDO (H-11) sc-137012? The difference in the isotypes between then (IgG2b for the former and IgG1 for the latter) changes something in the secondary antibody that should be used for incubation?

Asked by: laraZB
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

sc-53978， used to do WB，Dilution ratio please？

Asked by: Sueis
Thanks for your question. IDO (mIDO-48) is recommended for detection of IDO by Western Blotting (starting dilution 1:200, dilution range 1:100-1:1000). 感谢您的提问。IDO (mIDO-48)在WB实验方法中的推荐稀释比例是1:100-1:1000，起始稀释比例为1:200。
Answered by: Technical Support 9
Date published: 2020-06-12

Hi, we found three bands (close to 45 KDa) on our western blot after using this antibody (sample from rat brain). I wonder if it is different isoforms of IDO or something else. Was the full lenght protein it was raised against IDO1 or IDO2?

Asked by: JulianoK
Thank you for your question. We have not tested this rat monoclonal antibody in rat brain samples. However, we would recommend that you include a secondary antibody control on your Western blots (omit primary antibody) to identify any possible non-specific binding from your anti-rat IgG secondary antibody alone in your rat brain samples. The exact epitope recognized by this rat monoclonal antibody has not yet been determined. It was raised against IDO1, and not IDO2 (also designated INDOL1).
Answered by: Technical Support
Date published: 2019-01-26

Can you recommend a positive control to use with IDO Antibody (mIDO-48): sc-53978 for Western blot?

Asked by: jenni
We recommend MOLT-4: sc-2233, Raji: sc-364236 or or human lymph node tissue extract: sc-363768 whole cell lysates to use as positive controls as we have Western blot data showing specific detection of IDO in these cell lines.
Answered by: Technical Support 3
Date published: 2017-01-09
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Rated 5 out of 5 by from It works no problem!Clear, distinct band if you use a good secondary antibody.
Date published: 2022-08-02
Rated 1 out of 5 by from QuestionableTried this antibody multiple times on mouse whole brain, hippocampus, striatum, lung and spleen. A 45 kDa band is present in all tissues, including tissues from Ido1 knock out mice. We have even found a similar band in cells that do not express Ido1 mRNA by qPCR. Has anyone confirmed this reagents specificity using Ido1 deficient samples, or am I the only one with this problem.
Date published: 2021-05-18
Rated 5 out of 5 by from CLEAR BANDSThe bands are clear. But I got 2 at the target position which quite confused me. Has anyone met this before?
Date published: 2017-12-20
Rated 5 out of 5 by from 功能很出色申请了抗体小样，提取的是回肠末端蛋白，western跑出来条带比较清晰，基本没看到杂带，总体性能还不错。
Date published: 2017-09-07
Rated 5 out of 5 by from excellentI used in lung cancer cell line 2LL for WB and IF, showing good results.
Date published: 2017-08-22
Rated 5 out of 5 by from Work Well on WBWe used this IDO Antibody for RAW264.7 cell by WB and the results were specific and strong.
Date published: 2017-08-03
Rated 5 out of 5 by from very usefulIL10 antibody effect is good, immunohistochemical staining uniform, positive coloring significantly. Recommend you to buy, inexpensive, with 1:100 with the very good.
Date published: 2017-07-21
Rated 5 out of 5 by from Mouse & Human tissues look great!Last week we worked out this antibody using human and mouse normal and abnormal tissues. Dilution 1:100, HIER sodium citrate pH 6.0. All tissues are beautiful!
Date published: 2017-06-20
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IDO 抗体 (mIDO-48) is rated 4.8 out of 5 by 18.
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