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IDH2 Antibody (B-6) - Western Blotting - Image 361839
IDH2 抗体 (B-6): sc-374476. Caki-1 (A) 和 Hep G2 (B) 全细胞裂解液中 IDH2 表达的 Western 印迹分析.
IDH2 抗体 (B-6): sc-374476. Caki-1 (A) 和 Hep G2 (B) 全细胞裂解液中 IDH2 表达的 Western 印迹分析.

IDH2 抗体 (B-6): sc-374476

4.8(6)
寫評論提問

说明书
  • IDH2抗体(B-6)是小鼠单克隆IgG1 κ, 在8篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列71-110位于human物种的IDH2的内部区域内
  • IDH2 抗体 (B-6) 推荐用于WB, IP, IF 和 ELISA，检测human 来源的 IDH2
  • 抗IDH2抗体（B-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取IDH2 (B-6): sc-374476的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是IDH2 Antibody (B-6) for WB applications. 的首选辅助检测试剂，这些试剂现与IDH2 Antibody (B-6) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 IDH 抗体包括 和

IDH2抗体（B-6）是一种IgG1 κ小鼠单克隆IDH2抗体，可通过WB、IP、IF和ELISA检测人源IDH2。IDH2抗体（B-6）既可作为非共轭抗IDH2抗体形式，也可作为多种共轭形式的抗IDH2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。IDH2（异柠檬酸脱氢酶2（NADP+），线粒体）也被称为NADP+特异性ICDH；异柠檬酸脱氢酶，线粒体；以及草酰琥珀酸脱羧酶，是一种由人类IDH2基因编码的含有452个氨基酸的酶。IDH2属于异柠檬酸和异丙基苹果酸脱氢酶家族，含有两个核苷酸结合区。IDH2参与NADP+向NADPH的还原，并维持线粒体中谷胱甘肽（GSH）的供应。它被认为在中间代谢和能量产生中发挥作用。IDH2还与丙酮酸脱氢酶复合物紧密相关。IDH2作为同源二聚体存在于线粒体中，每个亚基可以结合一个镁离子或锰离子。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

IDH2 抗体 (B-6) 参考文献:

  1. 分离可补充线粒体 NAD（+）-异柠檬酸脱氢酶亚基 I 缺失突变体的荚膜组织胞浆菌 cDNA。  |  Johnson, CH. and McEwen, JE. 1999. Yeast. 15: 799-804. PMID: 10398348
  2. 清酒酵母的 NAD+ 依赖性异柠檬酸脱氢酶基因（IDH1, IDH2）断裂对清酒醪中有机酸成分的影响。  |  Asano, T., et al. 1999. J Biosci Bioeng. 88: 258-63. PMID: 16232608
  3. 线粒体异柠檬酸脱氢酶保护人神经母细胞瘤 SH-SY5Y 细胞免受氧化应激。  |  Kim, SJ., et al. 2007. J Neurosci Res. 85: 139-52. PMID: 17075901
  4. 将细胞质中的α-甘露糖苷酶（MANA）归入第 15 号染色体 q11 至 qter 区，并确认线粒体中的异柠檬酸脱氢酶（IDHM）归入第 15 号染色体 q11 至 qter 区。  |  Champion, MJ., et al. 1978. Cytogenet Cell Genet. 22: 498-502. PMID: 752528
  5. 淋巴细胞活化过程中人线粒体 NADP 依赖性异柠檬酸脱氢酶的表达。  |  Luo, H., et al. 1996. J Cell Biochem. 60: 495-507. PMID: 8707889
  6. 通过原位杂交将人类线粒体NAD+特异性异柠檬酸脱氢酶α亚基（IDH3A）基因定位到15q25.1-->q25.2。  |  Huh, TL., et al. 1996. Genomics. 32: 295-6. PMID: 8833160
  7. 通过原位杂交将人类线粒体 NADP(+)特异性异柠檬酸脱氢酶（IDH2）基因定位到 15q26.1。  |  Oh, IU., et al. 1996. Genomics. 38: 104-6. PMID: 8954790
  8. 将人类线粒体异柠檬酸脱氢酶（ICD-M，EC 1.1.1.41）基因分配到第 15 号染色体上。  |  Grzeschik, KH. 1976. Hum Genet. 34: 23-8. PMID: 965003
  9. 高等植物 NAD 依赖性异柠檬酸脱氢酶的分子特征：酵母突变体互补异构体结构的证据。  |  Lancien, M., et al. 1998. Plant J. 16: 325-33. PMID: 9881153

订购信息

产品名称产品编号规格价格数量收藏夹

IDH2 抗体 (B-6)

sc-374476
200 µg/ml
RMB2377.00

IDH2 (B-6): m-IgG Fc BP-HRP 套装

sc-525967
200 µg Ab; 10 µg BP
RMB2662.00

IDH2 (B-6): m-IgG1 BP-HRP 套装

sc-552051
200 µg Ab; 20 µg BP
RMB2662.00

IDH2 抗体 (B-6) AC

sc-374476 AC
500 µg/ml, 25% agarose
RMB3129.00

IDH2 抗体 (B-6) HRP

sc-374476 HRP
200 µg/ml
RMB2377.00

IDH2 抗体 (B-6) FITC

sc-374476 FITC
200 µg/ml
RMB2482.00

IDH2 抗体 (B-6) PE

sc-374476 PE
200 µg/ml
RMB2580.00

IDH2 抗体 (B-6) Alexa Fluor® 488

sc-374476 AF488
200 µg/ml
RMB2685.00

IDH2 抗体 (B-6) Alexa Fluor® 546

sc-374476 AF546
200 µg/ml
RMB2685.00

IDH2 抗体 (B-6) Alexa Fluor® 594

sc-374476 AF594
200 µg/ml
RMB2685.00

IDH2 抗体 (B-6) Alexa Fluor® 647

sc-374476 AF647
200 µg/ml
RMB2685.00

IDH2 抗体 (B-6) Alexa Fluor® 680

sc-374476 AF680
200 µg/ml
RMB2685.00

IDH2 抗体 (B-6) Alexa Fluor® 790

sc-374476 AF790
200 µg/ml
RMB2685.00

What is the recommended fixation method for immunofluorescence staining with IDH2 (B-6): sc-374476 monoclonal antibody?

Asked by: Dr Ninau Qelp
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from IDH2 works wellWestern Blot analysis of IDH2 was performed well at endogenous level.
Date published: 2021-05-10
Rated 5 out of 5 by from successful result,We usd it detect IDH2 for by WB,tried three times, very successful result, clear band at 43kda.The antibody dilution used 1:400.
Date published: 2018-01-27
Rated 5 out of 5 by from Very nice.After measurement, very strong and clean baseband data found in Western blot: CAKI-1 IDH2 (A) and (B) Hep G2 cell lysate.
Date published: 2017-02-09
Rated 5 out of 5 by from usefulWe use IDH2 to participate in the reduction of NADP + to NADPH and to maintain the supply of glutathione in the mitochondria, which is believed to play a role in intermediate metabolism and energy production, as well as tight association with pyruvate dehydrogenase complexes. IDH2 is present as a homodimer in the mitochondria, and can bind one magnesium or manganese ion per subunit.
Date published: 2017-02-03
Rated 5 out of 5 by from Produced excellent immunofluorescence mitochondrialProduced excellent immunofluorescence mitochondrial staining in formalin-fixed HepG2 cells. -SCBT QC
Date published: 2015-04-21
Rated 5 out of 5 by from Great Western Blot data of IDH2 expression in CakiGreat Western Blot data of IDH2 expression in Caki-1 whole cell lysate. -SCBT QC
Date published: 2013-01-08
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IDH2 抗体 (B-6) is rated 4.8 out of 5 by 6.
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