Date published: 2025-9-8
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gasdermin Antibody (H-6) - Western Blotting - Image 376555
gasdermin 抗体 (H-6): sc-376318. COLO 205 全细胞裂解液 (A) 和小鼠舌组织提取物 (B) 中 gasdermin 表达的 Western 印迹分析.
gasdermin 抗体 (H-6): sc-376318. COLO 205 全细胞裂解液 (A) 和小鼠舌组织提取物 (B) 中 gasdermin 表达的 Western 印迹分析.

gasdermin 抗体 (H-6): sc-376318

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说明书
  • gasdermin 抗体 H-6 是小鼠单克隆 IgG1 κ，gasdermin抗体, 在9篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列1-196位于human物种的gasdermin的N-terminus
  • gasdermin 抗体 (H-6) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 gasdermin A
  • 抗gasdermin抗体（H-6）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 另提供生物素偶联抗体，可用于WB、IHC(P)和ELISA实验
  • 关于如何获取gasdermin (H-6): sc-376318的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成gasdermin 抗体 (H-6)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 gasdermin 抗体包括 GSDMDC1

gasdermin抗体（H-6）是一种IgG1 κ小鼠单克隆gasdermin抗体（也称为gasdermin抗体），可通过WB、IP、IF和ELISA检测小鼠、大鼠和人源的gasdermin蛋白。gasdermin抗体（H-6）既有非偶联的抗gasdermin抗体形式，也有多种偶联形式的抗gasdermin抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Gasdermin，也称为GSDMA、GSDM或FKSG9，是一种含有445个氨基酸的蛋白质，位于细胞质的核周区域，属于gasdermin家族。Gasdermin主要在胃肠道组织中表达，也存在于皮肤和乳腺中，其功能是诱导细胞凋亡，被认为具有肿瘤抑制活性，特别是在胃癌细胞中。编码gasdermin的基因定位于人类17号染色体，该染色体占人类基因组的2.5%以上，编码1200多个基因。两个关键的肿瘤抑制基因与17号染色体相关，即p53和BRCA1。肿瘤抑制因子p53通过DNA修复与细胞死亡之间的平衡来调节细胞命运，对维持细胞遗传完整性是必需的。p53的功能障碍或表达丧失与恶性细胞生长和Li-Fraumeni综合征相关。与p53一样，BRCA1直接参与DNA修复，不过它被认为是早期乳腺癌和卵巢癌、结肠癌、前列腺癌和输卵管癌易感性的遗传决定因素。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

gasdermin 抗体 (H-6) 参考文献:

  1. 定位在小鼠第 11 号染色体上的 Gasdermin（Gsdm）主要在上消化道中表达，但在人类胃癌细胞中被显著抑制。  |  Saeki, N., et al. 2000. Mamm Genome. 11: 718-24. PMID: 10967128
  2. GSDML-GSDM基因座周围的进化重组热点与PPP1R1B-ERBB2-GRB7扩增片段周围的肿瘤基因组重组热点密切相关。  |  Katoh, M. and Katoh, M. 2004. Int J Oncol. 24: 757-63. PMID: 15010812
  3. 新型基因家族 Gsdm 的成员仅在皮肤和胃肠道上皮细胞中以高度组织特异性的方式表达。  |  Tamura, M., et al. 2007. Genomics. 89: 618-29. PMID: 17350798
  4. 在胃癌中经常被抑制的 GASDERMIN 是 LMO1 在 TGF-beta 依赖性凋亡信号中的一个靶点。  |  Saeki, N., et al. 2007. Oncogene. 26: 6488-98. PMID: 17471240
  5. 四个 GSDM 家族基因（GSDMA-D）在正常和恶性上消化道上皮细胞中的独特表达和功能。  |  Saeki, N., et al. 2009. Genes Chromosomes Cancer. 48: 261-71. PMID: 19051310
  6. Caspase-11裂解gasdermin D以产生非典型炎性体信号。  |  Kayagaki, N., et al. 2015. Nature. 526: 666-71. PMID: 26375259
  7. gasdermin 家族的孔隙形成活性和结构自抑制作用。  |  Ding, J., et al. 2016. Nature. 535: 111-6. PMID: 27281216
  8. 热坏死：Gasdermin介导的程序性坏死性细胞死亡。  |  Shi, J., et al. 2017. Trends Biochem Sci. 42: 245-254. PMID: 27932073
  9. 化疗药物通过caspase-3对气体释放蛋白的裂解诱导热解。  |  Wang, Y., et al. 2017. Nature. 547: 99-103. PMID: 28459430
  10. 气体释放蛋白D形成孔洞的分子机制。  |  Devant, P. and Kagan, JC. 2023. Nat Immunol. 24: 1064-1075. PMID: 37277654
  11. 气体释放蛋白和MLKL坏死细胞死亡效应：信号传导和疾病。  |  Lawlor, KE., et al. 2024. Immunity. 57: 429-445. PMID: 38479360
  12. 上皮内肥大细胞驱动气体释放蛋白C介导的2型免疫。  |  Yang, L., et al. 2024. Immunity. 57: 1056-1070.e5. PMID: 38614091

订购信息

产品名称产品编号规格价格数量收藏夹

gasdermin 抗体 (H-6)

sc-376318
200 µg/ml
RMB2377.00

gasdermin 抗体 (H-6) AC

sc-376318 AC
500 µg/ml, 25% agarose
RMB3129.00

gasdermin 抗体 (H-6) HRP

sc-376318 HRP
200 µg/ml
RMB2377.00

gasdermin 抗体 (H-6) FITC

sc-376318 FITC
200 µg/ml
RMB2482.00

gasdermin 抗体 (H-6) PE

sc-376318 PE
200 µg/ml
RMB2580.00

gasdermin 抗体 (H-6) Alexa Fluor® 488

sc-376318 AF488
200 µg/ml
RMB2685.00

gasdermin 抗体 (H-6) Alexa Fluor® 546

sc-376318 AF546
200 µg/ml
RMB2685.00

gasdermin 抗体 (H-6) Alexa Fluor® 594

sc-376318 AF594
200 µg/ml
RMB2685.00

gasdermin 抗体 (H-6) Alexa Fluor® 647

sc-376318 AF647
200 µg/ml
RMB2685.00

gasdermin 抗体 (H-6) Alexa Fluor® 680

sc-376318 AF680
200 µg/ml
RMB2685.00

gasdermin 抗体 (H-6) Alexa Fluor® 790

sc-376318 AF790
200 µg/ml
RMB2685.00

gasdermin 抗体 (H-6) B

sc-376318 B
200 µg/ml
RMB2407.00

Necesito el anticuerpo para usarlo en Inmunohistquimica. Tiene disponible el anticuerpo para esa técnica?

Asked by: Anonymous
Deberá ponerse en contacto con su distribuidor local. A continuación se muestra un enlace a su información de contacto. https://www.scbt.com/resources/distributors
Answered by: Technical Service
Date published: 2024-10-11

Is the gasdermin Antibody (H-6) recommended with m-IgG Fc BP-HRP, m-IgG1 BP-HRP or m-IgGκ BP-HRP by any chance?

Asked by: Fern
Thank you for your question. At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for gasdermin Antibody (H-6). This work is in progress.
Answered by: BlakeJ
Date published: 2024-07-22

For western blots, should I dilute the primary antibody in 5% w/v nonfat dry milk, 1X TBS, 0.1% Tween® 20 or just T-TBS? Thank you

Asked by: tonytony
Thank you for your question. For blocking we recommend UltraCruz® Blocking Reagent: sc-516214. Otherwise, milk-based blocking buffers are best suited for a broad blocking effect reducing unspecific signals, or BSA-based blocking if signals are specific but comparatively faint. Contact our technical service team directly for further assistance.
Answered by: Tech Support Europe
Date published: 2023-06-14

Does the antibody recognize cleaved form of GSDMD? Such as N-term (p30) and C-term (p23)? Thanks!

Asked by: tuuu
Thank you for the question. Gasdermin is a 445 amino acid protein This clone (H-6) was raised against amino acids 1-196 mapping at the N-terminus of human Gasdermin. Epitope mapping has not been carried out for this antibody. Therefore the exact binding region within the below sequence and reactivity with cleaved GSDMDs has not been determined, yet: MTMFENVTRALARQLNPRGDLTPLDSLIDFKRFHPFCLVLRKRKSTLFWGARYVRTDYTLLDVLEPGSSPSDPTDTGNFGFKNMLDTRVEGDVDVPKTVKVKGTAGLSQNSTLEVQTLSVAPKALETVQERKLAADHPFLKEMQDQGENLYVVMEVVETVQEVTLERAGKAEACFSLPFFAPLGLQGSINHKEAVT
Answered by: Technical Support Europe
Date published: 2017-12-27

What is the recommended fixation method for immunofluorescence staining with gasdermin (H-6): sc-376318 monoclonal antibody?

Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 3 out of 5 by from GasderminNice antibody-Gasdermin H-6 detects 53kd band with the mouse liver lysate-1:400 dil in 5% SM.
Date published: 2017-05-19
Rated 5 out of 5 by from This is the recommended gasdermin monoclonalWorks as expected in Western blotting and Immunofluoresence on mouse, rat an human samples
Date published: 2017-02-01
Rated 5 out of 5 by from Produced excellent immunofluorescence cytoplasmicProduced excellent immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-05-01
Rated 5 out of 5 by from Produced positive Western Blot data of gasderminProduced positive Western Blot data of gasdermin expression in non-transfected and mouse gasdermin transfected 293 whole cell lysates. -SCBT QC
Date published: 2013-09-11
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gasdermin 抗体 (H-6) is rated 4.5 out of 5 by 4.
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