Date published: 2025-9-10
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FXR/NR1H4 Antibody (D-3) - Western Blotting - Image 6584
FXR 抗体 (D-3): sc-25309. 人重组 FXR 融合蛋白的 Western 印迹分析 (A, B).
FXR/NR1H4 Antibody (D-3) - Western Blotting - Image 6584
FXR 抗体 (D-3): sc-25309. 人重组 FXR 融合蛋白的 Western 印迹分析 (A, B).
FXR/NR1H4 Antibody (D-3) - Western Blotting - Image 398964
FXR/NR1H4 抗体 (D-3): sc-25309. 人重组 FXR 的 Western 印迹分析. 所用检测试剂: m-IgGκ BP-HRP: sc-516102.
FXR/NR1H4 Antibody (D-3) - Western Blotting - Image 382261
FXR 抗体 (D-3): sc-25309. 小鼠回肠组织提取物中 FXR 表达的 Western 印迹分析.
FXR 抗体 (D-3): sc-25309. 人重组 FXR 融合蛋白的 Western 印迹分析 (A, B).

FXR/NR1H4 抗体 (D-3): sc-25309

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寫評論提問

说明书
  • FXR/NR1H4 抗体 D-3 是小鼠单克隆 IgG2a κ，FXR/NR1H4抗体, 在74篇文献中引用，规格为200 µg/ml
  • 免疫human物种的FXR的氨基酸1-130
  • FXR/NR1H4 抗体 (D-3) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 FXR
  • 抗FXR/NR1H4抗体（D-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-25309 X, 200 µg/0.1 ml)
  • 关于如何获取FXR/NR1H4 (D-3): sc-25309的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是FXR/NR1H4 Antibody (D-3) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与FXR/NR1H4 Antibody (D-3) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 FXR 抗体包括 FXR β

FXR抗体（D-3）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的FXR蛋白。抗FXR抗体（D-3）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。FXR（又称NR1H4）在调节胆汁酸平衡和脂质代谢中起着至关重要的作用，因此FXR对于维持代谢健康至关重要。FXR主要位于肝脏和肠道，作为核受体，它与配体结合后，可调节参与胆汁酸合成和转运的基因的表达。这种调节至关重要，因为胆汁酸失调会导致代谢紊乱、肝脏疾病和其他健康问题。FXR与类维生素X受体（RXR）形成异二聚体，并与称为反应元件的特定DNA序列结合，从而影响基因转录。FXR能够与包括法尼酯在内的各种脂质分子相互作用，这进一步凸显了FXR在代谢信号通路中的重要性。抗FXR抗体（D-3）具有多种应用，在代谢调节中发挥着关键作用，是研究脂质代谢和相关疾病复杂性的研究人员的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

FXR/NR1H4 抗体 (D-3) 参考文献:

  1. 胆汁酸核受体FXR与消化系统疾病。  |  Ding, L., et al. 2015. Acta Pharm Sin B. 5: 135-44. PMID: 26579439
  2. FXR调节肠道癌干细胞增殖。  |  Fu, T., et al. 2019. Cell. 176: 1098-1112.e18. PMID: 30794774
  3. FXR激活可通过胆汁酸依赖性脂质吸收减少来预防非酒精性脂肪肝。  |  Clifford, BL., et al. 2021. Cell Metab. 33: 1671-1684.e4. PMID: 34270928
  4. FXR：结构, 生物学以及治疗 NASH 和纤维化疾病的药物开发。  |  Tian, SY., et al. 2022. Acta Pharmacol Sin. 43: 1120-1132. PMID: 35217809
  5. FXR介导ILC对肠道炎症的内在反应。  |  Fu, T., et al. 2022. Proc Natl Acad Sci U S A. 119: e2213041119. PMID: 36508655
  6. FXR激动剂用于MASH疗法：从奥贝胆酸中汲取的经验和展望。  |  Wang, K., et al. 2024. Med Res Rev. 44: 568-586. PMID: 37899676
  7. 9-顺式维甲酸信号转导：更换合作伙伴令人兴奋。  |  Leblanc, BP. and Stunnenberg, HG. 1995. Genes Dev. 9: 1811-6. PMID: 7649469
  8. LXR 是一种核受体，它定义了一种独特的视黄醇反应途径。  |  Willy, PJ., et al. 1995. Genes Dev. 9: 1033-45. PMID: 7744246
  9. 鉴定一种可被法呢醇代谢物激活的核受体。  |  Forman, BM., et al. 1995. Cell. 81: 687-93. PMID: 7774010
  10. RXR, RAR 和 TR 的 DNA 结合域之间形成的二聚化界面决定了全长受体与直接重复序列的结合特异性和极性。  |  Zechel, C., et al. 1994. EMBO J. 13: 1425-33. PMID: 8137826
  11. 核受体超家族：第二个十年。  |  Mangelsdorf, DJ., et al. 1995. Cell. 83: 835-9. PMID: 8521507
  12. RXR 异二聚体和孤儿受体。  |  Mangelsdorf, DJ. and Evans, RM. 1995. Cell. 83: 841-50. PMID: 8521508

订购信息

产品名称产品编号规格价格数量收藏夹

FXR/NR1H4 抗体 (D-3)

sc-25309
200 µg/ml
RMB2377.00

FXR/NR1H4 (D-3): m-IgG Fc BP-HRP 套装

sc-528399
200 µg Ab; 10 µg BP
RMB2662.00

FXR/NR1H4 (D-3): m-IgGκ BP-HRP 套装

sc-520771
200 µg Ab, 40 µg BP
RMB2662.00

FXR/NR1H4 (D-3): m-IgG2a BP-HRP 套装

sc-547085
200 µg Ab; 10 µg BP
RMB2662.00

FXR/NR1H4 抗体 (D-3) X

sc-25309 X
200 µg/0.1 ml
RMB2377.00

FXR/NR1H4 抗体 (D-3) AC

sc-25309 AC
500 µg/ml, 25% agarose
RMB3129.00

FXR/NR1H4 抗体 (D-3) HRP

sc-25309 HRP
200 µg/ml
RMB2377.00

FXR/NR1H4 抗体 (D-3) FITC

sc-25309 FITC
200 µg/ml
RMB2482.00

FXR/NR1H4 抗体 (D-3) PE

sc-25309 PE
200 µg/ml
RMB2580.00

FXR/NR1H4 抗体 (D-3) Alexa Fluor® 488

sc-25309 AF488
200 µg/ml
RMB2685.00

FXR/NR1H4 抗体 (D-3) Alexa Fluor® 546

sc-25309 AF546
200 µg/ml
RMB2685.00

FXR/NR1H4 抗体 (D-3) Alexa Fluor® 594

sc-25309 AF594
200 µg/ml
RMB2685.00

FXR/NR1H4 抗体 (D-3) Alexa Fluor® 647

sc-25309 AF647
200 µg/ml
RMB2685.00

FXR/NR1H4 抗体 (D-3) Alexa Fluor® 680

sc-25309 AF680
200 µg/ml
RMB2685.00

FXR/NR1H4 抗体 (D-3) Alexa Fluor® 790

sc-25309 AF790
200 µg/ml
RMB2685.00

我用大鼠肝脏组织，在25-35kDa有很强的条带，在50kDa有较弱的条带，请问应该怎么办？

Asked by: csycsy
感谢您的提问。您可以拨打电话：021-60936350，发送邮件到[email protected]，或者我们官网上的在线聊天，联系我们的亚洲技术支持团队，了解更多信息。 Thank you for your question. Please contact our Technical Service Department for more information. Our Asian Technical Service team is available at (+86) 021-60936350 or [email protected] or by live chat on our website.
Answered by: Jiajun Z
Date published: 2024-07-30

请问可以跑小鼠肝脏组织吗

Asked by: Anonymous
感谢您的提问。 FXR/NR1H4 Antibody (D-3)推荐用于mouse,rat和human来源样本的WB, IP, IF和ELISA实验。 Thank you for your question. FXR/NR1H4 Antibody (D-3) is recommended for detection of FXR of mouse, rat and human origin by WB, IP, IF and ELISA.
Answered by: Ling Chen
Date published: 2024-06-18

Why GSTtagged human recombinant FXR is approximately 31/41KD

Asked by: Liu b
Thank you for your question. It would be helpful if you could call us directly at 800- 457-3801 option 2, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-08-04

Which FXR isoforms are detected？

Asked by: Cecily
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-23

Is it possible that we are seeing FXR at around 50kd rather than 75kd in the intestine using western blot analysis? I am aware from the data sheet that it says: Molecular Weight (predicted) 56 kDa. Molecular Weight (observed) 75 kDa. So i

Asked by: sian13
thank you for your question. Isoform 5 of FXR (identifier: Q96RI1-5) maps at about 50 kDa, so it is possible that this is the isoform you are detecting in your samples.
Answered by: Tech Support Europe
Date published: 2025-03-01

Can this antibody be used in mouse cell line for detection of FXR by western blotting?

Asked by: nancy011
Thank you for your question. We haven’t tested this antibody yet in mouse cell lines, and so that is not a recommended species at this time. However, there are product citations and customer reviews indicating this antibody can be used successfully for detection of mouse FXR by Western blotting.
Answered by: Technical Support
Date published: 2019-01-26

When should the TransCruz reagent sc-25309 X be used instead of the standard form sc-25309?

Asked by: jenniferc
Thank you for your question. We recommend using the TransCruz reagent form when performing ChIP or GelShift assays. This form can also be used in other applications such as Western Blot or IHC, but the dilution should be adjusted by a factor of 10 to account for the higher original concentration.
Answered by: Technical Support
Date published: 2017-02-24

Hi, There is any publication where this antibody was used in mouse tissue. I'm interested in staining FXR in mouse intestine. Thanks in advance

Asked by: Marcel Sorribas
Thank you for your question. We have not yet tested this antibody on mouse tissue. We are aware of a publication citing use of the antibody for Western blot of mouse liver samples. Please refer to the following reference on PubMed in Mol Cell Biol., PMID: 22290433. We have not yet tested this antibody for IHC on formalin-fixed paraffin-embedded tissue sections. It is currently recommended for IF, or immunofluorescence staining of cells or tissues on fresh/frozen tissue sections fixed in acetone or methanol. If you would be interested in testing a free 10 µg sample of this antibody, please contact our Technical Service Department. Please call us toll-free at (800) 457-3801 option 2, email [email protected] or use our live chat.
Answered by: Technical Support
Date published: 2017-02-10
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Rated 4 out of 5 by from 用的可以最近刚试着用了一下Santa 的这个抗体，孵育完了之后用发光液发了一下，感觉条带还可以，效果觉得课可以。
Date published: 2018-04-16
Rated 5 out of 5 by from GOODWestern blot analysis of rat liver tissue protein 1：600
Date published: 2017-10-12
Rated 3 out of 5 by from 只能显细胞过转的FXR；显示不了组织中的，组织中相应的条带是杂带。显示不了组织中的，组织中相应的条带是杂带，用了FXRKO鼠的组织做阴性对照，也在相应未知出现条带，但是293T细胞过表达FXR时能显示出过量表达的FXR。 故最后结论：只能显细胞FXR,不能显示组织FXR!
Date published: 2017-09-22
Rated 3 out of 5 by from FXR used for WBI applied for a sample two week ago.My sample is the mouse liver. Incubated the antibody for overnight at 4℃, 1:1000 dilution, and  m-IgGκ BP-HRP  incubated for 1 hour at RT , 1:1000 dilution.The results are shown in the figure.
Date published: 2017-09-16
Rated 5 out of 5 by from Very good results in WBVery clear WB band without any non-specific ones. Works well with liver samples and we'll check it out in other tissues.
Date published: 2017-08-30
Rated 5 out of 5 by from work well in IHC and IBI suggest use 1:500 dissolved in TBST containing 5% BSA for IB detection of HepG2 lysates. 1:50 was uited to IHC of liver tissues
Date published: 2017-07-05
Rated 5 out of 5 by from worked wellI suggest use 1:400 to 1:800 use 5% BSA dilution,it suitable for WB detect human spleen tissue lysates.
Date published: 2017-06-14
Rated 5 out of 5 by from Western blotWestern blot result data is very good, nice signal band, clear and no backgournd,
Date published: 2017-06-11
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FXR/NR1H4 抗体 (D-3) is rated 4.6 out of 5 by 20.
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