Date published: 2025-9-7
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FGF-2 Antibody (C-2) - Western Blotting - Image 379922
FGF-2 抗体 (C-2): sc-74412. WI-38 (A) 和 XP12RO (B) 全细胞裂解液中 FGF-2 表达的 Western 印迹分析.
FGF-2 抗体 (C-2): sc-74412. WI-38 (A) 和 XP12RO (B) 全细胞裂解液中 FGF-2 表达的 Western 印迹分析.

FGF-2 抗体 (C-2): sc-74412

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寫評論提問

说明书
  • FGF-2抗体(C-2)是小鼠单克隆IgG2a κ, 在37篇文献中引用，规格为200 µg/ml
  • 免疫human物种的FGF-2的氨基酸10-140
  • 建议使用FGF-2抗体（C-2）通过WB, IP, IF, IHC(P)和ELISA检测precursor and mature FGF-2的mouse, rat, human和Xenopus来源
  • 抗FGF-2抗体（C-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取FGF-2 (C-2): sc-74412的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 FGF-2 抗体 (C-2) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与FGF-2 抗体 (C-2) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 FGF 抗体 包括 FGF-1, FGF-10, FGF-12, FGF-15, FGF-16, FGF-17, FGF-18, FGF-19, FGF-2和FGF-20

FGF-2抗体（C-2）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的成纤维细胞生长因子-2（FGF-2）蛋白。抗FGF-2抗体（C-2）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。FGF-2通过促进细胞增殖和分化，在包括血管生成、伤口愈合和组织修复在内的各种生物过程中发挥着关键作用。FGF-2主要位于细胞外基质中，由多种细胞类型分泌，这一点非常重要，因为细胞外存在使其能够与靶细胞上的特定受体相互作用，从而启动信号通路，引发细胞反应。FGF-2与受体（属于受体酪氨酸激酶家族）的相互作用对于调节生物学效应至关重要，这使得FGF-2成为发育过程和癌症等病理条件中的关键因素。FGF-2能够刺激间充质细胞、上皮细胞和神经外胚层细胞的增殖，在正常生理和疾病状态下均具有重要意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

FGF-2 抗体 (C-2) 参考文献:

  1. 小鼠乳腺癌细胞依赖雄激素生长所必需的雄激素诱导生长因子的克隆和特征描述。  |  Tanaka, A., et al. 1992. Proc Natl Acad Sci U S A. 89: 8928-32. PMID: 1409588
  2. 克隆和表达与酸性和碱性成纤维细胞生长因子交叉反应的两种不同的高亲和力受体。  |  Dionne, CA., et al. 1990. EMBO J. 9: 2685-92. PMID: 1697263
  3. 碱性成纤维细胞生长因子细胞生物学的最新发展。  |  Rifkin, DB. and Moscatelli, D. 1989. J Cell Biol. 109: 1-6. PMID: 2545723
  4. 成纤维细胞生长因子家族新成员--与 HST 相关的 FGF.6 基因的特征。  |  Marics, I., et al. 1989. Oncogene. 4: 335-40. PMID: 2649847
  5. 通过转染卡波西肉瘤 DNA 分离出的癌基因编码一种生长因子，属于 FGF 家族。  |  Delli Bovi, P., et al. 1987. Cell. 50: 729-37. PMID: 2957062
  6. 小鼠 int-2 的序列, 拓扑结构和蛋白质编码潜力：一种由小鼠乳腺肿瘤病毒激活的假定癌基因。  |  Moore, R., et al. 1986. EMBO J. 5: 919-24. PMID: 3013624
  7. 人类 FGF-5 癌基因编码一种与成纤维细胞生长因子有关的新型蛋白质。  |  Zhan, X., et al. 1988. Mol Cell Biol. 8: 3487-95. PMID: 3211147
  8. 鼻咽癌中的FGF-2信号传导调节了周细胞-巨噬细胞之间的相互作用和转移。  |  Wang, Y., et al. 2022. JCI Insight. 7: PMID: 35439170
  9. 使用细胞点阵法研究翼状胬肉中的成纤维细胞生长因子-2（FGF-2）表达。  |  Mastronikolis, S., et al. 2022. Vision (Basel). 6: PMID: 36278670
  10. 在TGF-β诱导的EMT过程中，FGF-2可调节晶状体上皮细胞的行为。  |  Flokis, M. and Lovicu, FJ. 2023. Cells. 12: PMID: 36980168
  11. 编码成纤维细胞生长因子家族第九个成员的新型细胞因子 cDNA 的分子克隆，该细胞因子具有独特的分泌特性。  |  Miyamoto, M., et al. 1993. Mol Cell Biol. 13: 4251-9. PMID: 8321227
  12. 小鼠成纤维细胞生长因子 10：cDNA 克隆, 蛋白质鉴定和 mRNA 表达调控。  |  Beer, HD., et al. 1997. Oncogene. 15: 2211-8. PMID: 9393979

订购信息

产品名称产品编号规格价格数量收藏夹

FGF-2 抗体 (C-2)

sc-74412
200 µg/ml
RMB2377.00

FGF-2 (C-2): m-IgG Fc BP-HRP 套装

sc-525703
200 µg Ab; 10 µg BP
RMB2662.00

FGF-2 (C-2): m-IgG2a BP-HRP 套装

sc-546367
200 µg Ab; 10 µg BP
RMB2662.00

FGF-2 抗体 (C-2) AC

sc-74412 AC
500 µg/ml, 25% agarose
RMB3129.00

FGF-2 抗体 (C-2) HRP

sc-74412 HRP
200 µg/ml
RMB2377.00

FGF-2 抗体 (C-2) FITC

sc-74412 FITC
200 µg/ml
RMB2482.00

FGF-2 抗体 (C-2) PE

sc-74412 PE
200 µg/ml
RMB2580.00

FGF-2 抗体 (C-2) Alexa Fluor® 488

sc-74412 AF488
200 µg/ml
RMB2685.00

FGF-2 抗体 (C-2) Alexa Fluor® 546

sc-74412 AF546
200 µg/ml
RMB2685.00

FGF-2 抗体 (C-2) Alexa Fluor® 594

sc-74412 AF594
200 µg/ml
RMB2685.00

FGF-2 抗体 (C-2) Alexa Fluor® 647

sc-74412 AF647
200 µg/ml
RMB2685.00

FGF-2 抗体 (C-2) Alexa Fluor® 680

sc-74412 AF680
200 µg/ml
RMB2685.00

FGF-2 抗体 (C-2) Alexa Fluor® 790

sc-74412 AF790
200 µg/ml
RMB2685.00

Would the target of this antibody suggest that it would be able to successfully detect ferret FGF2?

Asked by: Amy R
Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Support
Date published: 2021-07-22

What blocking solution should I use with FGF-2 (C-2): sc-74412 monoclonal antibody for Western blot?

Asked by: jenniferc
Thank you for your question. We recommend blocking with Blotto (either TBS Blotto A: sc-2333 or TBS Blotto B: sc-2335) Our Western blot protocol can be found here: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Santa Cruz TS
Date published: 2017-01-31
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Rated 1 out of 5 by from Did not recognize rat FGF-2I tried this antibody out to detect rat FGF-2. I tested it by running it in a Western blot against recombinant rat FGF-2. This antibody did not detect the protein while other ones did. It did not detect either reduced or non reduced recombinant rat FGF-2.
Date published: 2018-11-14
Rated 5 out of 5 by from Excellent AntibodyWorks great for IF in mouse lungs. There is a bit of background, but may be due to mouse antibody on mouse tissue.
Date published: 2018-04-06
Rated 5 out of 5 by from Sharp bands on human VSMCsThe sample antibody gives very sharp bands and clean background
Date published: 2017-07-20
Rated 5 out of 5 by from very good detection of all FGF2 isoformsGreat detection of FGF2 isoforms (18, 22-22.5, 24KDa) in different human melanoma cell lines, but 34KDa isoform never detected. High sensitive and specific antibody. Used 1:1000 in WB.
Date published: 2017-05-17
Rated 5 out of 5 by from Good IP stainThis antibody gave us a good IP stain in the nuclear region of human cells
Date published: 2016-12-21
Rated 5 out of 5 by from Good specificity in WB in human normal foreskinGood specificity in WB in human normal foreskin samples. -SCBT Publication Review
Date published: 2015-04-23
Rated 5 out of 5 by from Strong band in WB in human recombinant FGFStrong band in WB in human recombinant FGF-2. -SCBT QC
Date published: 2015-04-21
Rated 5 out of 5 by from Produced exceptional immunoperoxidase nuclearProduced exceptional immunoperoxidase nuclear staining in formalin fixed, paraffin-embedded human brain tissue. -SCBT QC
Date published: 2015-02-03
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FGF-2 抗体 (C-2) is rated 4.6 out of 5 by 9.
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