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EWS Antibody (C-9) - Western Blotting - Image 303387 — EWS 抗体 (C-9): sc-48404. Jurkat (A), HeLa (B) 和 K-562 (C) 核提取物以及 K-562 (D), C32 (E) 和 HeLa (F) 全细胞裂解液中 EWS 表达的 Western 印迹分析.

EWS 抗体 (C-9): sc-48404. Jurkat (A), HeLa (B) 和 K-562 (C) 核提取物以及 K-562 (D), C32 (E) 和 HeLa (F) 全细胞裂解液中 EWS 表达的 Western 印迹分析.

EWS 抗体 (C-9): sc-48404

Name: EWS抗体 (C-9)
Brand: SCBIO
SKU: sc-48404
Price: 2377.0 CNY
Availability: InStock

4.4(7)

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说明书

EWS抗体(C-9)是小鼠单克隆IgG₁ κ, 在18篇文献中引用，规格为200 µg/ml
特异性抗原位于human物种的EWS的N-terminus的氨基酸2-19之间
EWS 抗体 (C-9) 推荐用于WB, IP, IF, IHC(P) 和 ELISA ，检测 mouse, rat, human 和来源的EWS; 也和以下物种反应，包括：和 canine, bovine, porcine and avian
抗EWS抗体（C-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
关于如何获取EWS (C-9): sc-48404的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG Fc BP-HRP 和 m-IgG₁ BP-HRP 是 EWS 抗体 (C-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与EWS 抗体 (C-9) 组合成套装提供。 (请参阅下面的订购信息)。

查看产品引用文献(18)

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関連項目

EWS抗体（C-9）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）等方法检测小鼠、大鼠和人类样本中的EWS。EWS抗体（C-9）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。EWS是一种关键的核RNA结合蛋白，在基因调控和细胞过程中发挥着重要作用。值得注意的是，EWS基因通常参与染色体易位，导致与转录因子（如ATF-1）融合蛋白的形成，这些蛋白与人类肿瘤的形成有关。在尤因肉瘤中，EWS蛋白与ETS转录因子的DNA结合结构域融合，包括Fli-1、Erg、ETV1、E1AF和FEV，形成EWS/Fli-1嵌合蛋白。这种融合蛋白是一种强效转录激活因子，可增强靶基因的表达并促进细胞转化，这对肿瘤的发生至关重要。EWS蛋白包含两个功能区域：氨基末端区域（A区）具有有限的转激活活性，但与Fli-1融合后可有效诱导转化；远端区域（B区）具有转激活活性，但与Fli-1结合后促进转化的效果较差。了解EWS的功能动态及其相互作用对于阐明其在癌症生物学中的作用和制定靶向治疗策略至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor^® 是Molecular Probes Inc., OR., USA的商标

LI-COR^®和 Odyssey^® 是LI-COR Biosciences的注册商标

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
EWS 抗体 (C-9)	sc-48404	200 µg/ml	RMB2377.00
EWS (C-9): m-IgG Fc BP-HRP 套装	sc-526750	200 µg Ab; 10 µg BP	RMB2662.00
EWS (C-9): m-IgG₁ BP-HRP 套装	sc-532123	200 µg Ab; 20 µg BP	RMB2662.00
EWS 抗体 (C-9) AC	sc-48404 AC	500 µg/ml, 25% agarose	RMB3129.00
EWS 抗体 (C-9) HRP	sc-48404 HRP	200 µg/ml	RMB2377.00
EWS 抗体 (C-9) FITC	sc-48404 FITC	200 µg/ml	RMB2482.00
EWS 抗体 (C-9) PE	sc-48404 PE	200 µg/ml	RMB2580.00
EWS 抗体 (C-9) Alexa Fluor^® 488	sc-48404 AF488	200 µg/ml	RMB2685.00
EWS 抗体 (C-9) Alexa Fluor^® 546	sc-48404 AF546	200 µg/ml	RMB2685.00
EWS 抗体 (C-9) Alexa Fluor^® 594	sc-48404 AF594	200 µg/ml	RMB2685.00
EWS 抗体 (C-9) Alexa Fluor^® 647	sc-48404 AF647	200 µg/ml	RMB2685.00
EWS 抗体 (C-9) Alexa Fluor^® 680	sc-48404 AF680	200 µg/ml	RMB2685.00
EWS 抗体 (C-9) Alexa Fluor^® 790	sc-48404 AF790	200 µg/ml	RMB2685.00
EWS (C-9) 中和勝肽	sc-48404 P	100 µg/0.5 ml	RMB511.00

I am looking at C9 antibody for EWSR1 and also for EWS-FLI1 fusion protein detection in Ewing Sarcoma. it appears this Ab detects AA 2-19 but the first citation states it is detecting an epitope AA 431 to 490. Conspicuously, ab G5 is for AA 2-19.

Asked by: Professor Ewing

For detailed assistance please contact Technical Service

Answered by: Tech Sales

Date published: 2024-08-14

Can the peptide sc-48404 P be used as a positive control in Western Blot?

Asked by: AbPolly

Thank you for your question. The blocking peptide sc-48404 P is a short synthetic peptide intended for use as a negative control. It is not appropriate for use as a positive control in Western Blot.

Answered by: Technical Support 8

Date published: 2017-01-26

Rated 5 out of 5 by Professor Griffin from Nice signalNice strong signal produced with EWS Antibody (C-9) in multiple applications and samples

Date published: 2017-01-19

Rated 5 out of 5 by TS from Produced exceptional immunofluorescence nuclearProduced exceptional immunofluorescence nuclear staining in formalin-fixed HeLa cells. -SCBT QC

Date published: 2015-05-16

Rated 4 out of 5 by ET from Publishable data in CoIP using A673 cellsPublishable data in CoIP using A673 cells -SCBT Publication Review

Date published: 2015-04-11

Rated 4 out of 5 by SM from Worked well with transfected lysate by WBWorked well with transfected lysate by WB. -SCBT QC

Date published: 2015-03-24

Rated 4 out of 5 by AM from Strong bands seen in WB using KStrong bands seen in WB using K-562 and F9 whole cell lysates and rat testis tissue extract. -SCBT QC

Date published: 2015-02-12

Rated 4 out of 5 by ET from Publishable data in IP using human EwingPublishable data in IP using human Ewing sarcoma cell lines A673, SK-N-MC, and RD-ES -SCBT Publication Review

Date published: 2015-01-08

Rated 5 out of 5 by Jenny from This EWS antibody works very well for westernThis EWS antibody works very well for western (1:2000), IF (1:200), IHC (1:33), and ChIP.

Date published: 2012-05-28

EWS 抗体 (C-9) is rated 4.4 out of 5 by 7.

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种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	EWSR1	Entrez Gene ID: 2130	22q12.2	XM_005261389, XM_005261390, XM_011529995, XM_011529996, XM_011529997, XM_011529998, XM_011529999, XM_011530000, XM_011530001, XM_011530002, XM_017028644, XM_017028645, XM_017028646, XM_017028647, XM_017028648, XM_017028649, XM_017028650, XM_017028651, XM_017028652, XM_017028653, XM_017028654, XM_017028655, XM_017028656, XM_017028657, XM_017028658, XM_017028659, XM_017028660, XM_017028661, XM_017028662, XM_017028663, XM_017028664, XM_017028665, XM_017028666, NM_001163285, NM_001163286, NM_001163287, NM_005243, NM_013986	UniProt ID: Q01844	133450
Mouse	Ewsr1	Entrez Gene ID: 14030	11 A1	XM_006514508, XM_006514509, XM_006514510, XM_006514511, XM_006514512, XM_017314255, NM_001283061, NM_001283062, NM_001283063, NM_007968	UniProt ID: Q61545	N/A

EWS 抗体 (C-9): sc-48404

EWS 抗体 (C-9) 规格

替代产品名称: EWSR1

产品克隆名称: C-9

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG₁ kappa light chain

氨基酸: 2-19

抗原表位靶标: EWS

抗原表位种属: human

产品反应性/检测: EWS

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: canine, bovine, porcine and avian

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, IHC(P), ELISA

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 18篇各种科学出版物的引文

優選的 EWS 抗體是 EWS 抗體 (G-5): sc-28327.

EWS 抗体 (C-9)

EWS (C-9): m-IgG Fc BP-HRP 套装

EWS (C-9): m-IgG₁ BP-HRP 套装

EWS 抗体 (C-9) AC

EWS 抗体 (C-9) HRP

EWS 抗体 (C-9) FITC

EWS 抗体 (C-9) PE

EWS 抗体 (C-9) Alexa Fluor^® 488

EWS 抗体 (C-9) Alexa Fluor^® 546

EWS 抗体 (C-9) Alexa Fluor^® 594

EWS 抗体 (C-9) Alexa Fluor^® 647

EWS 抗体 (C-9) Alexa Fluor^® 680

EWS 抗体 (C-9) Alexa Fluor^® 790

EWS (C-9) 中和勝肽

I am looking at C9 antibody for EWSR1 and also for EWS-FLI1 fusion protein detection in Ewing Sarcoma. it appears this Ab detects AA 2-19 but the first citation states it is detecting an epitope AA 431 to 490. Conspicuously, ab G5 is for AA 2-19.

Can the peptide sc-48404 P be used as a positive control in Western Blot?

EWS 抗体 (C-9): sc-48404

EWS 抗体 (C-9) 规格

替代产品名称: EWSR1

产品克隆名称: C-9

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG1 kappa light chain

氨基酸: 2-19

抗原表位靶标: EWS

抗原表位种属: human

产品反应性/检测: EWS

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: canine, bovine, porcine and avian

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, IHC(P), ELISA

瓶的信息: Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 18篇各种科学出版物的引文

EWS 抗体 (C-9)可选的偶联物/形式: AC: Agarose, AF488: Alexa Fluor® 488, AF546: Alexa Fluor® 546, AF594: Alexa Fluor® 594, AF647: Alexa Fluor® 647, AF680: Alexa Fluor® 680, AF790: Alexa Fluor® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin

優選的 EWS 抗體是 EWS 抗體 (G-5): sc-28327.

EWS 抗体 (C-9)

EWS (C-9): m-IgG Fc BP-HRP 套装

EWS (C-9): m-IgG1 BP-HRP 套装

EWS 抗体 (C-9) AC

EWS 抗体 (C-9) HRP

EWS 抗体 (C-9) FITC

EWS 抗体 (C-9) PE

EWS 抗体 (C-9) Alexa Fluor® 488

EWS 抗体 (C-9) Alexa Fluor® 546

EWS 抗体 (C-9) Alexa Fluor® 594

EWS 抗体 (C-9) Alexa Fluor® 647

EWS 抗体 (C-9) Alexa Fluor® 680

EWS 抗体 (C-9) Alexa Fluor® 790

EWS (C-9) 中和勝肽

I am looking at C9 antibody for EWSR1 and also for EWS-FLI1 fusion protein detection in Ewing Sarcoma. it appears this Ab detects AA 2-19 but the first citation states it is detecting an epitope AA 431 to 490. Conspicuously, ab G5 is for AA 2-19.

Can the peptide sc-48404 P be used as a positive control in Western Blot?

抗体同型: IgG₁ kappa light chain

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

EWS (C-9): m-IgG₁ BP-HRP 套装

EWS 抗体 (C-9) Alexa Fluor^® 488

EWS 抗体 (C-9) Alexa Fluor^® 546

EWS 抗体 (C-9) Alexa Fluor^® 594

EWS 抗体 (C-9) Alexa Fluor^® 647

EWS 抗体 (C-9) Alexa Fluor^® 680

EWS 抗体 (C-9) Alexa Fluor^® 790